AUX1_ARATH
ID AUX1_ARATH Reviewed; 485 AA.
AC Q96247;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Auxin transporter protein 1;
DE AltName: Full=Auxin influx carrier protein 1;
DE AltName: Full=Polar auxin transport inhibitor-resistant protein 1;
GN Name=AUX1; Synonyms=AUX, PIR1, WAV5; OrderedLocusNames=At2g38120;
GN ORFNames=F16M14.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, AND
RP MUTAGENESIS OF GLY-459.
RX PubMed=8688077; DOI=10.1126/science.273.5277.948;
RA Bennett M.J., Marchant A., Green H.G., May S.T., Ward S.P., Millner P.A.,
RA Walker A.R., Schultz B., Feldmann K.A.;
RT "Arabidopsis AUX1 gene: a permease-like regulator of root gravitropism.";
RL Science 273:948-950(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RA Mirza J.I., Olsen G.M., Iversen T.-H., Maher E.P.;
RT "The growth and gravitropic responses of wild-type and auxin-resistant
RT mutants of Arabidopsis thaliana.";
RL Physiol. Plantarum 60:516-522(1984).
RN [7]
RP FUNCTION.
RX PubMed=16667854; DOI=10.1104/pp.94.3.1462;
RA Pickett F.B., Wilson A.K., Estelle M.A.;
RT "The aux1 mutation of Arabidopsis confers both auxin and ethylene
RT resistance.";
RL Plant Physiol. 94:1462-1466(1990).
RN [8]
RP FUNCTION.
RA Okada K., Shimura Y.;
RT "Reversible root tip rotation in Arabidopsis seedlings induced by obstacle-
RT touching stimulus.";
RL Science 250:274-276(1990).
RN [9]
RP FUNCTION.
RX PubMed=8278539; DOI=10.1104/pp.102.3.983;
RA Cao Y., Glass A.D.M., Crawford N.M.;
RT "Ammonium inhibition of Arabidopsis root growth can be reversed by
RT potassium and by auxin resistance mutations aux1, axr1, and axr2.";
RL Plant Physiol. 102:983-989(1993).
RN [10]
RP FUNCTION.
RA Evans M.L., Ishikawa H., Estelle M.A.;
RT "Responses of Arabidopsis roots to auxin studied with high temporal
RT resolution: comparison of wild type and auxin-response mutants.";
RL Planta 194:215-222(1994).
RN [11]
RP FUNCTION.
RX PubMed=11536712; DOI=10.1046/j.1365-313x.1995.8040561.x;
RA Timpte C., Lincoln C., Pickett F.B., Turner J., Estelle M.A.;
RT "The AXR1 and AUX1 genes of Arabidopsis function in separate auxin-response
RT pathways.";
RL Plant J. 8:561-569(1995).
RN [12]
RP FUNCTION.
RX PubMed=7768447; DOI=10.1093/genetics/139.3.1393;
RA Roman G., Lubarsky B., Kieber J.J., Rothenberg M., Ecker J.R.;
RT "Genetic analysis of ethylene signal transduction in Arabidopsis thaliana:
RT five novel mutant loci integrated into a stress response pathway.";
RL Genetics 139:1393-1409(1995).
RN [13]
RP FUNCTION.
RX PubMed=9032965; DOI=10.1093/oxfordjournals.pcp.a029059;
RA Fujita H., Syono K.;
RT "Genetic analysis of the effects of polar auxin transport inhibitors on
RT root growth in Arabidopsis thaliana.";
RL Plant Cell Physiol. 37:1094-1101(1996).
RN [14]
RP FUNCTION.
RX PubMed=9697346; DOI=10.1093/oxfordjournals.pcp.a029419;
RA Yamamoto M., Yamamoto K.T.;
RT "Differential effects of 1-naphthaleneacetic acid, indole-3-acetic acid and
RT 2,4-dichlorophenoxyacetic acid on the gravitropic response of roots in an
RT auxin-resistant mutant of Arabidopsis, aux1.";
RL Plant Cell Physiol. 39:660-664(1998).
RN [15]
RP FUNCTION.
RX PubMed=10036773; DOI=10.1046/j.1365-313x.1998.00321.x;
RA Pitts R.J., Cernac A., Estelle M.A.;
RT "Auxin and ethylene promote root hair elongation in Arabidopsis.";
RL Plant J. 16:553-560(1998).
RN [16]
RP FUNCTION, AND MUTAGENESIS OF GLY-79; GLY-247 AND ALA-272.
RX PubMed=9484486; DOI=10.1023/a:1005961303167;
RA Marchant A., Bennett M.J.;
RT "The Arabidopsis AUX1 gene: a model system to study mRNA processing in
RT plants.";
RL Plant Mol. Biol. 36:463-471(1998).
RN [17]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=10205161; DOI=10.1093/emboj/18.8.2066;
RA Marchant A., Kargul J., May S.T., Muller P., Delbarre A.,
RA Perrot-Rechenmann C., Bennett M.J.;
RT "AUX1 regulates root gravitropism in Arabidopsis by facilitating auxin
RT uptake within root apical tissues.";
RL EMBO J. 18:2066-2073(1999).
RN [18]
RP FUNCTION, GENE FAMILY, AND NOMENCLATURE.
RX DOI=10.1007/s003440010030;
RA Parry P.G., Marchant A., May S.T., Swarup R., Swarup K., James N.,
RA Graham N., Allen T., Martucci T., Yemm A., Napier R., Manning K., King G.,
RA Bennett M.J.;
RT "Quick on the uptake: characterization of a family of plant auxin influx
RT carriers.";
RL J. Plant Growth Regul. 20:217-225(2001).
RN [19]
RP FUNCTION.
RX PubMed=11266581; DOI=10.1093/pcp/pce035;
RA Rahman A., Amakawa T., Goto N., Tsurumi S.;
RT "Auxin is a positive regulator for ethylene-mediated response in the growth
RT of Arabidopsis roots.";
RL Plant Cell Physiol. 42:301-307(2001).
RN [20]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=11641271; DOI=10.1101/gad.210501;
RA Swarup R., Friml J., Marchant A., Ljung K., Sandberg G., Palme K.,
RA Bennett M.J.;
RT "Localization of the auxin permease AUX1 suggests two functionally distinct
RT hormone transport pathways operate in the Arabidopsis root apex.";
RL Genes Dev. 15:2648-2653(2001).
RN [21]
RP FUNCTION, AND ACTIVITY REGULATION.
RX PubMed=11260496; DOI=10.1046/j.1365-313x.2001.00970.x;
RA Parry G., Delbarre A., Marchant A., Swarup R., Napier R.,
RA Perrot-Rechenmann C., Bennett M.J.;
RT "Novel auxin transport inhibitors phenocopy the auxin influx carrier
RT mutation aux1.";
RL Plant J. 25:399-406(2001).
RN [22]
RP ACTIVITY REGULATION.
RX PubMed=11161055; DOI=10.1104/pp.125.2.990;
RA Rahman A., Ahamed A., Amakawa T., Goto N., Tsurumi S.;
RT "Chromosaponin I specifically interacts with AUX1 protein in regulating the
RT gravitropic response of Arabidopsis roots.";
RL Plant Physiol. 125:990-1000(2001).
RN [23]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11864575; DOI=10.1016/s0960-9822(02)00654-1;
RA Grebe M., Friml J., Swarup R., Ljung K., Sandberg G., Terlou M., Palme K.,
RA Bennett M.J., Scheres B.;
RT "Cell polarity signaling in Arabidopsis involves a BFA-sensitive auxin
RT influx pathway.";
RL Curr. Biol. 12:329-334(2002).
RN [24]
RP FUNCTION.
RX PubMed=12481073; DOI=10.1104/pp.010546;
RA Rahman A., Hosokawa S., Oono Y., Amakawa T., Goto N., Tsurumi S.;
RT "Auxin and ethylene response interactions during Arabidopsis root hair
RT development dissected by auxin influx modulators.";
RL Plant Physiol. 130:1908-1917(2002).
RN [25]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=11910006; DOI=10.1105/tpc.010354;
RA Marchant A., Bhalerao R., Casimiro I., Ekloef J., Casero P.J.,
RA Bennett M.J., Sandberg G.;
RT "AUX1 promotes lateral root formation by facilitating indole-3-acetic acid
RT distribution between sink and source tissues in the Arabidopsis seedling.";
RL Plant Cell 14:589-597(2002).
RN [26]
RP FUNCTION, TOPOLOGY, SUBCELLULAR LOCATION, AND MUTAGENESIS OF SER-57;
RP GLY-178; SER-215; GLY-238; ALA-250; MET-259; PRO-262; GLY-292; MET-305;
RP THR-325; SER-386 AND GLY-439.
RX PubMed=15486104; DOI=10.1105/tpc.104.024737;
RA Swarup R., Kargul J., Marchant A., Zadik D., Rahman A., Mills R., Yemm A.,
RA May S., Williams L., Millner P., Tsurumi S., Moore I., Napier R.,
RA Kerr I.D., Bennett M.J.;
RT "Structure-function analysis of the presumptive Arabidopsis auxin permease
RT AUX1.";
RL Plant Cell 16:3069-3083(2004).
CC -!- FUNCTION: Carrier protein involved in proton-driven auxin influx.
CC Mediates the formation of auxin gradient from developing leaves (site
CC of auxin biosynthesis) to tips by contributing to the loading of auxin
CC in vascular tissues and facilitating acropetal (base to tip) auxin
CC transport within inner tissues of the root apex, and basipetal (tip to
CC base) auxin transport within outer tissues of the root apex. Unloads
CC auxin from the mature phloem to deliver the hormone to the root
CC meristem via the protophloem cell files. Coordinated subcellular
CC localization of AUX1 is regulated by a brefeldin A-sensitive (BFA)
CC vesicle trafficking process. Involved in lateral root formation,
CC trichoblast polarization and root hair elongation. Required for
CC gravitropism and thigmotropism, especially in roots, by modulating
CC responses to auxin, ethylene and cytokinins such as benzyladenine (BA).
CC Needed for ammonium-mediated root-growth inhibition. Confers
CC sensitivity to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D,
CC auxin analog), and to polar auxin transport inhibitors such as N-1-
CC naphthylphthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA).
CC {ECO:0000269|PubMed:10036773, ECO:0000269|PubMed:10205161,
CC ECO:0000269|PubMed:11260496, ECO:0000269|PubMed:11266581,
CC ECO:0000269|PubMed:11536712, ECO:0000269|PubMed:11641271,
CC ECO:0000269|PubMed:11864575, ECO:0000269|PubMed:11910006,
CC ECO:0000269|PubMed:12481073, ECO:0000269|PubMed:15486104,
CC ECO:0000269|PubMed:16667854, ECO:0000269|PubMed:7768447,
CC ECO:0000269|PubMed:8278539, ECO:0000269|PubMed:8688077,
CC ECO:0000269|PubMed:9032965, ECO:0000269|PubMed:9484486,
CC ECO:0000269|PubMed:9697346, ECO:0000269|Ref.10, ECO:0000269|Ref.18,
CC ECO:0000269|Ref.6, ECO:0000269|Ref.8}.
CC -!- ACTIVITY REGULATION: Auxin uptake mediated by AUX1 is inhibited by
CC chromosaponin-1 (CSI), 1-naphthoxyacetic acid (1-NOA) and 3-chloro-4-
CC hydroxyphenylacetic acid (CHPAA). {ECO:0000269|PubMed:11161055,
CC ECO:0000269|PubMed:11260496}.
CC -!- INTERACTION:
CC Q96247; Q9FZ33: AXR4; NbExp=5; IntAct=EBI-16935343, EBI-25514394;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11641271,
CC ECO:0000269|PubMed:11864575, ECO:0000269|PubMed:15486104}; Multi-pass
CC membrane protein {ECO:0000269|PubMed:11641271,
CC ECO:0000269|PubMed:11864575, ECO:0000269|PubMed:15486104}. Note=In S2
CC columella cells, a dynamic cytoplasmic to membrane localization seems
CC to occur during early stage of gravity signal transduction. In roots
CC protophloem cells, asymmetric repartition in the upper plasma membrane.
CC -!- TISSUE SPECIFICITY: Expressed in root and shoot apical tissues. In root
CC apex, confined to stele initials, protophloem poles, statolith-
CC containing S2 columella cells, lateral root cap cells (LRC), and in
CC epidermal cells from the distal elongation zone (DEZ) up to central
CC elongation zone (CEZ). {ECO:0000269|PubMed:10205161,
CC ECO:0000269|PubMed:11641271, ECO:0000269|PubMed:8688077}.
CC -!- DEVELOPMENTAL STAGE: In seedlings, first expressed in primary root,
CC followed by shoot apical meristem and then in lateral roots. During
CC lateral root formation, expression in primordia starts at a late phase
CC of stage I, before the first periclinal division. In later stages II
CC and III, localized in the apical tip, extending to the elongation zone,
CC and in the vascular cylinder extending to the junction with the primary
CC root. {ECO:0000269|PubMed:11910006}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.1) subfamily.
CC {ECO:0000305}.
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DR EMBL; X98772; CAA67308.1; -; Genomic_DNA.
DR EMBL; AC003028; AAC27161.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09491.1; -; Genomic_DNA.
DR EMBL; AY054488; AAK96679.1; -; mRNA.
DR EMBL; AY093300; AAM13299.1; -; mRNA.
DR EMBL; AY087092; AAM64652.1; -; mRNA.
DR PIR; T01244; T01244.
DR RefSeq; NP_565882.1; NM_129368.3.
DR AlphaFoldDB; Q96247; -.
DR SMR; Q96247; -.
DR BioGRID; 3734; 39.
DR IntAct; Q96247; 36.
DR STRING; 3702.AT2G38120.1; -.
DR TCDB; 2.A.18.1.1; the amino acid/auxin permease (aaap) family.
DR PaxDb; Q96247; -.
DR PRIDE; Q96247; -.
DR ProteomicsDB; 240930; -.
DR EnsemblPlants; AT2G38120.1; AT2G38120.1; AT2G38120.
DR GeneID; 818390; -.
DR Gramene; AT2G38120.1; AT2G38120.1; AT2G38120.
DR KEGG; ath:AT2G38120; -.
DR Araport; AT2G38120; -.
DR TAIR; locus:2005496; AT2G38120.
DR eggNOG; KOG1303; Eukaryota.
DR HOGENOM; CLU_027994_2_0_1; -.
DR InParanoid; Q96247; -.
DR OMA; WAAMYVL; -.
DR OrthoDB; 509343at2759; -.
DR PhylomeDB; Q96247; -.
DR PRO; PR:Q96247; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q96247; baseline and differential.
DR Genevisible; Q96247; AT.
DR GO; GO:0009986; C:cell surface; IDA:TAIR.
DR GO; GO:0005768; C:endosome; IDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; ISS:TAIR.
DR GO; GO:0010011; F:auxin binding; IDA:TAIR.
DR GO; GO:0010328; F:auxin influx transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0009926; P:auxin polar transport; TAS:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0001736; P:establishment of planar polarity; IGI:TAIR.
DR GO; GO:0010311; P:lateral root formation; IMP:TAIR.
DR GO; GO:0009958; P:positive gravitropism; IMP:TAIR.
DR GO; GO:0009624; P:response to nematode; HEP:TAIR.
DR GO; GO:0048829; P:root cap development; IMP:TAIR.
DR GO; GO:0048765; P:root hair cell differentiation; IGI:TAIR.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Auxin signaling pathway; Cell membrane; Membrane;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..485
FT /note="Auxin transporter protein 1"
FT /id="PRO_0000093841"
FT TOPO_DOM 1..59
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 78..79
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 101..135
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 157..172
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 194..196
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 218..232
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 254..266
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..287
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 288..314
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 336..356
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 378
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 379..399
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 400..425
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 426..446
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 447..485
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 12..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 57
FT /note="S->F: In aux1-120; agravitropism."
FT /evidence="ECO:0000269|PubMed:15486104"
FT MUTAGEN 79
FT /note="G->R: In aux1-110; resistance to 2,4-D."
FT /evidence="ECO:0000269|PubMed:9484486"
FT MUTAGEN 178
FT /note="G->D: In aux1-112; agravitropism."
FT /evidence="ECO:0000269|PubMed:15486104"
FT MUTAGEN 215
FT /note="S->F: In aux1-111; agravitropism."
FT /evidence="ECO:0000269|PubMed:15486104"
FT MUTAGEN 238
FT /note="G->E: In aux1-104; agravitropism."
FT /evidence="ECO:0000269|PubMed:15486104"
FT MUTAGEN 247
FT /note="G->E: In aux1-106; resistance to 2,4-D,
FT agravitropism."
FT /evidence="ECO:0000269|PubMed:9484486"
FT MUTAGEN 250
FT /note="A->T: In aux1-113; partial agravitropism."
FT /evidence="ECO:0000269|PubMed:15486104"
FT MUTAGEN 259
FT /note="M->I: In aux1-114; partial agravitropism."
FT /evidence="ECO:0000269|PubMed:15486104"
FT MUTAGEN 262
FT /note="P->S: In aux1-116; partial agravitropism."
FT /evidence="ECO:0000269|PubMed:15486104"
FT MUTAGEN 272
FT /note="A->V: In aux1-2; resistance to 2,4-D, lower growth
FT rate of roots and hypocotyls, partial gravitropism."
FT /evidence="ECO:0000269|PubMed:9484486"
FT MUTAGEN 292
FT /note="G->E: In aux1-117; agravitropism."
FT /evidence="ECO:0000269|PubMed:15486104"
FT MUTAGEN 305
FT /note="M->R: In aux1-102; agravitropism."
FT /evidence="ECO:0000269|PubMed:15486104"
FT MUTAGEN 325
FT /note="T->M: In aux1-103; agravitropism."
FT /evidence="ECO:0000269|PubMed:15486104"
FT MUTAGEN 386
FT /note="S->N: In aux1-118; agravitropism."
FT /evidence="ECO:0000269|PubMed:15486104"
FT MUTAGEN 439
FT /note="G->R: In aux1-105; agravitropism."
FT /evidence="ECO:0000269|PubMed:15486104"
FT MUTAGEN 459
FT /note="G->D: In aux1-7; agravitropism, resistance to
FT ethylene, auxin and 2,4-D."
FT /evidence="ECO:0000269|PubMed:8688077"
SQ SEQUENCE 485 AA; 54060 MW; 6A9C22D362853C34 CRC64;
MSEGVEAIVA NDNGTDQVNG NRTGKDNEEH DGSTGSNLSN FLWHGGSVWD AWFSCASNQV
AQVLLTLPYS FSQLGMLSGI VLQIFYGLLG SWTAYLISVL YVEYRARKEK EGKSFKNHVI
QWFEVLDGLL GSYWKALGLA FNCTFLLFGS VIQLIACASN IYYINDHLDK RTWTYIFGAC
CATTVFIPSF HNYRIWSFLG LGMTTYTAWY LAIASIIHGQ AEGVKHSGPT KLVLYFTGAT
NILYTFGGHA VTVEIMHAMW KPQKFKYIYL MATLYVFTLT IPSAAAVYWA FGDALLDHSN
AFSLMPKNAW RDAAVILMLI HQFITFGFAC TPLYFVWEKV IGMHDTKSIC LRALARLPVV
IPIWFLAIIF PFFGPINSAV GALLVSFTVY IIPSLAHMLT YRSASARQNA AEKPPFFMPS
WTAMYVLNAF VVVWVLIVGF GFGGWASVTN FVRQVDTFGL FAKCYQCKPA AAAAHAPVSA
LHHRL
