ID   AV240_PHYSO             Reviewed;         194 AA.
AC   E0W4T1;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 1.
DT   25-MAY-2022, entry version 26.
DE   RecName: Full=RxLR effector protein Avh240 {ECO:0000303|PubMed:21653195};
DE   AltName: Full=Avirulence homolog protein 240 {ECO:0000303|PubMed:21653195};
DE   Flags: Precursor;
GN   Name=Avh240 {ECO:0000303|PubMed:21653195};
OS   Phytophthora sojae (Soybean stem and root rot agent) (Phytophthora
OS   megasperma f. sp. glycines).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=67593;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION, FUNCTION, INDUCTION, AND
RP   DOMAIN.
RC   STRAIN=P7064, P7074, and P7076;
RX   PubMed=21653195; DOI=10.1105/tpc.111.086082;
RA   Wang Q., Han C., Ferreira A.O., Yu X., Ye W., Tripathy S., Kale S.D.,
RA   Gu B., Sheng Y., Sui Y., Wang X., Zhang Z., Cheng B., Dong S., Shan W.,
RA   Zheng X., Dou D., Tyler B.M., Wang Y.;
RT   "Transcriptional programming and functional interactions within the
RT   Phytophthora sojae RXLR effector repertoire.";
RL   Plant Cell 23:2064-2086(2011).
CC   -!- FUNCTION: Effector that suppresses plant defense responses during the
CC       early stages of pathogen infection (PubMed:21653195). Suppresses cell
CC       death induced by effectors and PAMPs in plant hosts (PubMed:21653195).
CC       Avh240 dimerizes and localizes at the plasma membrane to interfere with
CC       aspartic protease AP1 secretion, which presents an effective mechanism
CC       by which effector proteins suppress plant apoplastic immunity (By
CC       similarity). {ECO:0000250|UniProtKB:G5A8M1,
CC       ECO:0000269|PubMed:21653195}.
CC   -!- SUBUNIT: Homodimer (By similarity). Interacts with host soybean
CC       aspartic protease AP1 (By similarity). {ECO:0000250|UniProtKB:G5A8M1}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:G5A8M1}. Host
CC       cell membrane {ECO:0000250|UniProtKB:G5A8M1}. Note=Plasma membrane
CC       localization is independent on self-dimerization but required for
CC       virulence. {ECO:0000250|UniProtKB:G5A8M1}.
CC   -!- INDUCTION: Expression is strongly up-regulated during the early stages
CC       of infection. {ECO:0000269|PubMed:21653195}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:21653195}.
CC   -!- DOMAIN: The alpha 1 and alpha 2 helices (residues 58-108) are required
CC       for the localization the plasma membrane.
CC       {ECO:0000250|UniProtKB:G5A8M1}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; JN254191; AEK81004.1; -; Genomic_DNA.
DR   EMBL; JN254192; AEK81005.1; -; Genomic_DNA.
DR   EMBL; JN254193; AEK81006.1; -; Genomic_DNA.
DR   RefSeq; XP_009536419.1; XM_009538124.1.
DR   AlphaFoldDB; E0W4T1; -.
DR   SMR; E0W4T1; -.
DR   GeneID; 20640723; -.
DR   KEGG; psoj:PHYSODRAFT_288823; -.
DR   VEuPathDB; FungiDB:PHYSODRAFT_288823; -.
DR   HOGENOM; CLU_1258286_0_0_1; -.
DR   OMA; TNEHARH; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Host cell membrane; Host membrane; Membrane; Secreted; Signal; Virulence.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..194
FT                   /note="RxLR effector protein Avh240"
FT                   /id="PRO_5007653096"
FT   REGION          58..108
FT                   /note="Host plasma membrane-binding"
FT                   /evidence="ECO:0000250|UniProtKB:G5A8M1"
FT   MOTIF           38..57
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:21653195"
SQ   SEQUENCE   194 AA;  22225 MW;  AC0C5C064CDDCC39 CRC64;
     MRPYFTLLLA LAFILACTNL VEADAGRVLE TTTNEHARHL RTAVASVVDL PDDEDERLLG
     YNTVQLWRMR RTANKLMNGK LTTQKEAALK KWMASQQDKF LAKWLKSSSV YPDQVYSKLG
     LTKLGASAKS SPNYQLYEKY TEALLQRWTN FKASPDTVYK SLRLDKLGAK APQSPSYPMY
     EKYLQTFFRN QPAN