RR5_SPIOL
ID RR5_SPIOL Reviewed; 308 AA.
AC Q9ST69; A0A0K9QCC0; P82131;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=30S ribosomal protein S5, chloroplastic {ECO:0000303|PubMed:10874039};
DE AltName: Full=Chloroplastic small ribosomal subunit protein uS5c {ECO:0000303|PubMed:28007896};
DE Flags: Precursor;
GN Name=rps5; ORFNames=SOVF_195380;
OS Spinacia oleracea (Spinach).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Melody; TISSUE=Leaf;
RA Trifa Y., Diederich L., Lerbs-Mache S.;
RL Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Viroflay; TISSUE=Leaf;
RX PubMed=24352233; DOI=10.1038/nature12817;
RA Dohm J.C., Minoche A.E., Holtgraewe D., Capella-Gutierrez S.,
RA Zakrzewski F., Tafer H., Rupp O., Soerensen T.R., Stracke R., Reinhardt R.,
RA Goesmann A., Kraft T., Schulz B., Stadler P.F., Schmidt T., Gabaldon T.,
RA Lehrach H., Weisshaar B., Himmelbauer H.;
RT "The genome of the recently domesticated crop plant sugar beet (Beta
RT vulgaris).";
RL Nature 505:546-549(2014).
RN [3]
RP PROTEIN SEQUENCE OF 56-87; 115-126; 148-160; 168-173; 184-190; 196-209;
RP 211-218; 220-222; 227-253; 255-268 AND 300-306.
RA Yamaguchi K., von Knoblauch K., Subramanian A.R.;
RL Submitted (OCT-1999) to UniProtKB.
RN [4]
RP PROTEIN SEQUENCE OF 56-87, SUBUNIT, SUBCELLULAR LOCATION, AND MASS
RP SPECTROMETRY.
RC STRAIN=cv. Alwaro; TISSUE=Leaf;
RX PubMed=10874039; DOI=10.1074/jbc.m004350200;
RA Yamaguchi K., von Knoblauch K., Subramanian A.R.;
RT "The plastid ribosomal proteins. Identification of all the proteins in the
RT 30S subunit of an organelle ribosome (chloroplast).";
RL J. Biol. Chem. 275:28455-28465(2000).
RN [5]
RP STRUCTURE BY ELECTRON MICROSCOPY (9.4 ANGSTROMS).
RX PubMed=18042701; DOI=10.1073/pnas.0709856104;
RA Sharma M.R., Wilson D.N., Datta P.P., Barat C., Schluenzen F., Fucini P.,
RA Agrawal R.K.;
RT "Cryo-EM study of the spinach chloroplast ribosome reveals the structural
RT and functional roles of plastid-specific ribosomal proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:19315-19320(2007).
RN [6]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.40 ANGSTROMS), SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RX PubMed=28007896; DOI=10.15252/embj.201695959;
RA Bieri P., Leibundgut M., Saurer M., Boehringer D., Ban N.;
RT "The complete structure of the chloroplast 70S ribosome in complex with
RT translation factor pY.";
RL EMBO J. 36:475-486(2017).
CC -!- FUNCTION: Component of the chloroplast ribosome (chloro-ribosome), a
CC dedicated translation machinery responsible for the synthesis of
CC chloroplast genome-encoded proteins, including proteins of the
CC transcription and translation machinery and components of the
CC photosynthetic apparatus. {ECO:0000305|PubMed:10874039,
CC ECO:0000305|PubMed:28007896}.
CC -!- SUBUNIT: Component of the chloroplast small ribosomal subunit (SSU).
CC Mature 70S chloroplast ribosomes of higher plants consist of a small
CC (30S) and a large (50S) subunit. The 30S small subunit contains 1
CC molecule of ribosomal RNA (16S rRNA) and 24 different proteins. The 50S
CC large subunit contains 3 rRNA molecules (23S, 5S and 4.5S rRNA) and 33
CC different proteins (PubMed:10874039, PubMed:28007896). uS5c binds
CC directly to 16S ribosomal RNA (Ref.3). {ECO:0000269|PubMed:10874039,
CC ECO:0000269|PubMed:28007896, ECO:0000269|Ref.3}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:10874039, ECO:0000269|PubMed:28007896}.
CC -!- MASS SPECTROMETRY: Mass=27730; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:10874039};
CC -!- MASS SPECTROMETRY: Mass=27784; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:10874039};
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS5 family.
CC {ECO:0000305}.
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DR EMBL; X93159; CAA63650.1; -; mRNA.
DR EMBL; KQ197116; KNA04905.1; -; Genomic_DNA.
DR PDB; 4V61; EM; 9.40 A; AE=1-308.
DR PDB; 5MMJ; EM; 3.65 A; e=1-308.
DR PDB; 5MMM; EM; 3.40 A; e=1-308.
DR PDB; 5X8P; EM; 3.40 A; e=56-308.
DR PDB; 5X8R; EM; 3.70 A; e=56-308.
DR PDB; 6ERI; EM; 3.00 A; BE=151-308.
DR PDBsum; 4V61; -.
DR PDBsum; 5MMJ; -.
DR PDBsum; 5MMM; -.
DR PDBsum; 5X8P; -.
DR PDBsum; 5X8R; -.
DR PDBsum; 6ERI; -.
DR AlphaFoldDB; Q9ST69; -.
DR SMR; Q9ST69; -.
DR STRING; 3562.Q9ST69; -.
DR PRIDE; Q9ST69; -.
DR OrthoDB; 1504411at2759; -.
DR EvolutionaryTrace; Q9ST69; -.
DR Proteomes; UP000054095; Unassembled WGS sequence.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005763; C:mitochondrial small ribosomal subunit; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IEA:UniProt.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR Gene3D; 3.30.230.10; -; 1.
DR HAMAP; MF_01307_B; Ribosomal_S5_B; 1.
DR InterPro; IPR000851; Ribosomal_S5.
DR InterPro; IPR005712; Ribosomal_S5_bac-type.
DR InterPro; IPR005324; Ribosomal_S5_C.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR InterPro; IPR013810; Ribosomal_S5_N.
DR InterPro; IPR018192; Ribosomal_S5_N_CS.
DR PANTHER; PTHR13718; PTHR13718; 1.
DR Pfam; PF00333; Ribosomal_S5; 1.
DR Pfam; PF03719; Ribosomal_S5_C; 1.
DR SUPFAM; SSF54211; SSF54211; 1.
DR TIGRFAMs; TIGR01021; rpsE_bact; 1.
DR PROSITE; PS00585; RIBOSOMAL_S5; 1.
DR PROSITE; PS50881; S5_DSRBD; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antibiotic resistance; Chloroplast;
KW Direct protein sequencing; Plastid; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein; RNA-binding; rRNA-binding; Transit peptide.
FT TRANSIT 1..55
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:10874039, ECO:0000269|Ref.3"
FT CHAIN 56..308
FT /note="30S ribosomal protein S5, chloroplastic"
FT /id="PRO_0000248272"
FT DOMAIN 152..215
FT /note="S5 DRBM"
FT CONFLICT 59
FT /note="K -> T (in Ref. 2; KNA04905)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 308 AA; 33581 MW; EE8E29B8D43E1CB1 CRC64;
MATTATTTPS ATSLTTLHRR IPLFPTTTTL LSLSSSSKPL FLSLSSTRSF PTHLYCIKKD
DIDITFFEQD NPDEEITFDP PEKPEGYIPP RAVDEPPFES EEEIALAYEE LYGAAYSGES
LLGNDVYAMD SKIKKATGFG SKSKKEKIRD GFEENVVQVR RVTKVVKGGK HMRFRAIVVV
GDKKGQVGVG VGKAKEVVSA VQKAAVDARR NIITVPMTKY LTFPHRNEAD YGAARVMLRP
AAPGTGVIAG GAVRTVLEMA GVENALGKQL GSNNALNNAR ATIVAVQTMR QFSDVARDRG
IPMEELWK
