ID   AV241_PHYSP             Reviewed;         188 AA.
AC   G5A8D2;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   25-JAN-2012, sequence version 1.
DT   25-MAY-2022, entry version 27.
DE   RecName: Full=RxLR effector protein Avh241 {ECO:0000303|PubMed:21653195};
DE   AltName: Full=Avirulence homolog protein 241 {ECO:0000303|PubMed:21653195};
DE   Flags: Precursor;
GN   Name=Avh241 {ECO:0000303|PubMed:21653195}; ORFNames=PHYSODRAFT_288810;
OS   Phytophthora sojae (strain P6497) (Soybean stem and root rot agent)
OS   (Phytophthora megasperma f. sp. glycines).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=1094619;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=P6497;
RX   PubMed=16946064; DOI=10.1126/science.1128796;
RA   Tyler B.M., Tripathy S., Zhang X., Dehal P., Jiang R.H.Y., Aerts A.,
RA   Arredondo F.D., Baxter L., Bensasson D., Beynon J.L., Chapman J.,
RA   Damasceno C.M.B., Dorrance A.E., Dou D., Dickerman A.W., Dubchak I.L.,
RA   Garbelotto M., Gijzen M., Gordon S.G., Govers F., Grunwald N.J., Huang W.,
RA   Ivors K.L., Jones R.W., Kamoun S., Krampis K., Lamour K.H., Lee M.-K.,
RA   McDonald W.H., Medina M., Meijer H.J.G., Nordberg E.K., Maclean D.J.,
RA   Ospina-Giraldo M.D., Morris P.F., Phuntumart V., Putnam N.H., Rash S.,
RA   Rose J.K.C., Sakihama Y., Salamov A.A., Savidor A., Scheuring C.F.,
RA   Smith B.M., Sobral B.W.S., Terry A., Torto-Alalibo T.A., Win J., Xu Z.,
RA   Zhang H., Grigoriev I.V., Rokhsar D.S., Boore J.L.;
RT   "Phytophthora genome sequences uncover evolutionary origins and mechanisms
RT   of pathogenesis.";
RL   Science 313:1261-1266(2006).
RN   [2]
RP   IDENTIFICATION, AND DOMAIN.
RX   PubMed=21653195; DOI=10.1105/tpc.111.086082;
RA   Wang Q., Han C., Ferreira A.O., Yu X., Ye W., Tripathy S., Kale S.D.,
RA   Gu B., Sheng Y., Sui Y., Wang X., Zhang Z., Cheng B., Dong S., Shan W.,
RA   Zheng X., Dou D., Tyler B.M., Wang Y.;
RT   "Transcriptional programming and functional interactions within the
RT   Phytophthora sojae RXLR effector repertoire.";
RL   Plant Cell 23:2064-2086(2011).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, DOMAIN, AND MUTAGENESIS OF 73-GLY--LYS-78.
RX   PubMed=22816601; DOI=10.1111/j.1469-8137.2012.04241.x;
RA   Yu X., Tang J., Wang Q., Ye W., Tao K., Duan S., Lu C., Yang X., Dong S.,
RA   Zheng X., Wang Y.;
RT   "The RxLR effector Avh241 from Phytophthora sojae requires plasma membrane
RT   localization to induce plant cell death.";
RL   New Phytol. 196:247-260(2012).
CC   -!- FUNCTION: Effector that triggers cell death in a variety of plant
CC       species (including tobacco, tomato and soybean), regardless of the Rps
CC       genes present (PubMed:22816601). Avh241 interacts with the plant immune
CC       system via at least two different mechanisms, one recognized by plants
CC       dependent on subcellular localization and one promoting infection
CC       independent on membrane localization (PubMed:22816601). The cell death
CC       triggered by Avh241 in N.benthamiana requires the two host mitogen-
CC       activated protein kinases, MEK2 and WIPK (PubMed:22816601).
CC       {ECO:0000269|PubMed:22816601}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22816601}. Host cell
CC       membrane {ECO:0000269|PubMed:22816601}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:21653195}.
CC   -!- DOMAIN: The GAAKAK motif at position 73-78 is required for host plasma
CC       membrane localization. {ECO:0000269|PubMed:22816601}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; JH159161; EGZ08158.1; -; Genomic_DNA.
DR   RefSeq; XP_009536330.1; XM_009538035.1.
DR   AlphaFoldDB; G5A8D2; -.
DR   SMR; G5A8D2; -.
DR   EnsemblProtists; EGZ08158; EGZ08158; PHYSODRAFT_288810.
DR   GeneID; 20640716; -.
DR   KEGG; psoj:PHYSODRAFT_288810; -.
DR   InParanoid; G5A8D2; -.
DR   OMA; WIEAWAN; -.
DR   Proteomes; UP000002640; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR031825; RXLR.
DR   Pfam; PF16810; RXLR; 1.
PE   1: Evidence at protein level;
KW   Host cell membrane; Host membrane; Membrane; Reference proteome; Secreted;
KW   Signal; Virulence.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   CHAIN           17..188
FT                   /note="RxLR effector protein Avh241"
FT                   /id="PRO_5003473069"
FT   MOTIF           43..58
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:21653195"
FT   MOTIF           73..78
FT                   /note="Host plasma membrane localization motif"
FT                   /evidence="ECO:0000269|PubMed:22816601"
FT   MUTAGEN         73..78
FT                   /note="GAAKAK->AAAAAA: Impairs the localization to the host
FT                   plasma membrane and lowers cell death-inducing activity."
FT                   /evidence="ECO:0000269|PubMed:22816601"
SQ   SEQUENCE   188 AA;  21594 MW;  2CF64E963FBD9CDF CRC64;
     MRQYCLLLIV LALAAALSDN AVASTLAVAR SEIGAPSAVY SERLLRSEPQ DEDTFEDRAF
     GLNWLRLRWL RLGAAKAKTT DVISARESKW IEAWANKNLS PNYVYKQLGL AKQGDKAMQS
     QNYRIFEAYT ERLFAKDQAL YTKWLDAKMT PEDVYKALKL DKLLGAKAAN SPDFRRYEVY
     MFKWHELN