RR6_SPIOL
ID RR6_SPIOL Reviewed; 211 AA.
AC P82403; A0A0K9R0H2; P82404; P82405; P82406; P82407; Q9M4W2;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 3.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=30S ribosomal protein S6 alpha, chloroplastic {ECO:0000303|PubMed:10874039};
DE AltName: Full=Chloroplastic small ribosomal subunit protein bS6c {ECO:0000303|PubMed:28007896};
DE Contains:
DE RecName: Full=30S ribosomal protein S6 beta, chloroplastic {ECO:0000303|PubMed:10874039};
DE Contains:
DE RecName: Full=30S ribosomal protein S6 gamma, chloroplastic {ECO:0000303|PubMed:10874039};
DE Contains:
DE RecName: Full=30S ribosomal protein S6 delta, chloroplastic {ECO:0000303|PubMed:10874039};
DE Contains:
DE RecName: Full=30S ribosomal protein S6 epsilon, chloroplastic {ECO:0000303|PubMed:10874039};
DE Flags: Precursor;
GN Name=RPS6; ORFNames=SOVF_120630;
OS Spinacia oleracea (Spinach).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Viroflay; TISSUE=Leaf;
RX PubMed=24352233; DOI=10.1038/nature12817;
RA Dohm J.C., Minoche A.E., Holtgraewe D., Capella-Gutierrez S.,
RA Zakrzewski F., Tafer H., Rupp O., Soerensen T.R., Stracke R., Reinhardt R.,
RA Goesmann A., Kraft T., Schulz B., Stadler P.F., Schmidt T., Gabaldon T.,
RA Lehrach H., Weisshaar B., Himmelbauer H.;
RT "The genome of the recently domesticated crop plant sugar beet (Beta
RT vulgaris).";
RL Nature 505:546-549(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 44-211, PROTEIN SEQUENCE OF 66-100; 119-130
RP AND 139-150, SUBUNIT, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC STRAIN=cv. Alwaro; TISSUE=Leaf;
RX PubMed=10874039; DOI=10.1074/jbc.m004350200;
RA Yamaguchi K., von Knoblauch K., Subramanian A.R.;
RT "The plastid ribosomal proteins. Identification of all the proteins in the
RT 30S subunit of an organelle ribosome (chloroplast).";
RL J. Biol. Chem. 275:28455-28465(2000).
RN [3]
RP STRUCTURE BY ELECTRON MICROSCOPY (9.4 ANGSTROMS).
RX PubMed=18042701; DOI=10.1073/pnas.0709856104;
RA Sharma M.R., Wilson D.N., Datta P.P., Barat C., Schluenzen F., Fucini P.,
RA Agrawal R.K.;
RT "Cryo-EM study of the spinach chloroplast ribosome reveals the structural
RT and functional roles of plastid-specific ribosomal proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:19315-19320(2007).
RN [4]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.40 ANGSTROMS), SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RX PubMed=28007896; DOI=10.15252/embj.201695959;
RA Bieri P., Leibundgut M., Saurer M., Boehringer D., Ban N.;
RT "The complete structure of the chloroplast 70S ribosome in complex with
RT translation factor pY.";
RL EMBO J. 36:475-486(2017).
CC -!- FUNCTION: Component of the chloroplast ribosome (chloro-ribosome), a
CC dedicated translation machinery responsible for the synthesis of
CC chloroplast genome-encoded proteins, including proteins of the
CC transcription and translation machinery and components of the
CC photosynthetic apparatus. {ECO:0000305|PubMed:10874039,
CC ECO:0000305|PubMed:28007896}.
CC -!- SUBUNIT: Component of the chloroplast small ribosomal subunit (SSU).
CC Mature 70S chloroplast ribosomes of higher plants consist of a small
CC (30S) and a large (50S) subunit. The 30S small subunit contains 1
CC molecule of ribosomal RNA (16S rRNA) and 24 different proteins. The 50S
CC large subunit contains 3 rRNA molecules (23S, 5S and 4.5S rRNA) and 33
CC different proteins. {ECO:0000269|PubMed:10874039,
CC ECO:0000269|PubMed:28007896}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:10874039, ECO:0000269|PubMed:28007896}.
CC -!- MASS SPECTROMETRY: [30S ribosomal protein S6 alpha, chloroplastic]:
CC Mass=16326.7; Method=Electrospray; Note=S6 alpha form.;
CC Evidence={ECO:0000269|PubMed:10874039};
CC -!- MASS SPECTROMETRY: [30S ribosomal protein S6 beta, chloroplastic]:
CC Mass=15953; Method=Electrospray; Note=S6 beta form.;
CC Evidence={ECO:0000269|PubMed:10874039};
CC -!- MASS SPECTROMETRY: [30S ribosomal protein S6 epsilon, chloroplastic]:
CC Mass=13853.6; Method=Electrospray; Note=S6 epsilon form.;
CC Evidence={ECO:0000269|PubMed:10874039};
CC -!- MASS SPECTROMETRY: [30S ribosomal protein S6 alpha, chloroplastic]:
CC Mass=16423; Method=MALDI; Note=S6 alpha form.;
CC Evidence={ECO:0000269|PubMed:10874039};
CC -!- MASS SPECTROMETRY: [30S ribosomal protein S6 beta, chloroplastic]:
CC Mass=15951; Method=MALDI; Note=S6 beta form.;
CC Evidence={ECO:0000269|PubMed:10874039};
CC -!- MASS SPECTROMETRY: [30S ribosomal protein S6 epsilon, chloroplastic]:
CC Mass=13865; Method=MALDI; Note=S6 epsilon form.;
CC Evidence={ECO:0000269|PubMed:10874039};
CC -!- MISCELLANEOUS: 5 different forms exist, S6 alpha, S6 beta, S6 gamma, S6
CC delta and S6 epsilon, possibly due to different transit peptide
CC cleavage. {ECO:0000269|PubMed:10874039}.
CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bS6 family.
CC {ECO:0000305}.
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DR EMBL; KQ154025; KNA13015.1; -; Genomic_DNA.
DR EMBL; AF250383; AAF64311.1; -; mRNA.
DR PDB; 4V61; EM; 9.40 A; AF=44-211.
DR PDB; 5MMJ; EM; 3.65 A; f=1-211.
DR PDB; 5MMM; EM; 3.40 A; f=1-211.
DR PDB; 5X8P; EM; 3.40 A; f=66-211.
DR PDB; 5X8R; EM; 3.70 A; f=66-211.
DR PDB; 6ERI; EM; 3.00 A; BF=99-211.
DR PDBsum; 4V61; -.
DR PDBsum; 5MMJ; -.
DR PDBsum; 5MMM; -.
DR PDBsum; 5X8P; -.
DR PDBsum; 5X8R; -.
DR PDBsum; 6ERI; -.
DR AlphaFoldDB; P82403; -.
DR SMR; P82403; -.
DR STRING; 3562.P82403; -.
DR PRIDE; P82403; -.
DR OrthoDB; 1408893at2759; -.
DR EvolutionaryTrace; P82403; -.
DR Proteomes; UP000054095; Unassembled WGS sequence.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR GO; GO:0070181; F:small ribosomal subunit rRNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:InterPro.
DR Gene3D; 3.30.70.60; -; 1.
DR HAMAP; MF_00360; Ribosomal_S6; 1.
DR InterPro; IPR000529; Ribosomal_S6.
DR InterPro; IPR020814; Ribosomal_S6_plastid/chlpt.
DR InterPro; IPR035980; Ribosomal_S6_sf.
DR InterPro; IPR014717; Transl_elong_EF1B/ribosomal_S6.
DR PANTHER; PTHR21011; PTHR21011; 1.
DR Pfam; PF01250; Ribosomal_S6; 1.
DR SUPFAM; SSF54995; SSF54995; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Plastid;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW rRNA-binding; Transit peptide.
FT TRANSIT 1..65
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:10874039"
FT CHAIN 66..211
FT /note="30S ribosomal protein S6 alpha, chloroplastic"
FT /id="PRO_0000250217"
FT CHAIN 71..211
FT /note="30S ribosomal protein S6 beta, chloroplastic"
FT /id="PRO_0000250218"
FT CHAIN 77..211
FT /note="30S ribosomal protein S6 gamma, chloroplastic"
FT /id="PRO_0000250219"
FT CHAIN 86..211
FT /note="30S ribosomal protein S6 delta, chloroplastic"
FT /id="PRO_0000250220"
FT CHAIN 91..211
FT /note="30S ribosomal protein S6 epsilon, chloroplastic"
FT /id="PRO_0000250221"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 80..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 211 AA; 23484 MW; DBEFC1F7732D544D CRC64;
MATFSLTSTL PSSSPTTSLH SIPKPKLKPS TINFTHNLNP FNPNLKPSRH HYLSNYGPYV
KAIALDFSGS FFEGGFGGLD EDPPSTPPAG LAVEEKPEPQ CPPGLRQYET MAVLRPDMTE
DERLTLTQKY EELLVAGGAM YVEVFNRGVI PLAYSIKRKN KAGETNNYLD GIYLLFTYFT
KPESISPLEA ALVTDDDVIR SSSFKIRKRK Y
