ID   AV262_PHYSO             Reviewed;         123 AA.
AC   E0W5H5;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 1.
DT   25-MAY-2022, entry version 27.
DE   RecName: Full=RxLR effector protein Avh262 {ECO:0000303|PubMed:21653195};
DE   AltName: Full=Avirulence homolog protein 262 {ECO:0000303|PubMed:21653195};
DE   Flags: Precursor;
GN   Name=Avh262 {ECO:0000303|PubMed:21653195};
OS   Phytophthora sojae (Soybean stem and root rot agent) (Phytophthora
OS   megasperma f. sp. glycines).
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=67593;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION, AND DOMAIN.
RC   STRAIN=P7064, P7074, and P7076;
RX   PubMed=21653195; DOI=10.1105/tpc.111.086082;
RA   Wang Q., Han C., Ferreira A.O., Yu X., Ye W., Tripathy S., Kale S.D.,
RA   Gu B., Sheng Y., Sui Y., Wang X., Zhang Z., Cheng B., Dong S., Shan W.,
RA   Zheng X., Dou D., Tyler B.M., Wang Y.;
RT   "Transcriptional programming and functional interactions within the
RT   Phytophthora sojae RXLR effector repertoire.";
RL   Plant Cell 23:2064-2086(2011).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, DOMAIN, MUTAGENESIS OF 60-LEU--THR-82,
RP   INTERACTION WITH HOST BIP1; BIP2; BIP3 AND BIP4, AND SUBCELLULAR LOCATION.
RX   PubMed=27256489; DOI=10.1038/ncomms11685;
RA   Jing M., Guo B., Li H., Yang B., Wang H., Kong G., Zhao Y., Xu H., Wang Y.,
RA   Ye W., Dong S., Qiao Y., Tyler B.M., Ma W., Wang Y.;
RT   "A Phytophthora sojae effector suppresses endoplasmic reticulum stress-
RT   mediated immunity by stabilizing plant Binding immunoglobulin Proteins.";
RL   Nat. Commun. 7:11685-11685(2016).
CC   -!- FUNCTION: Effector that suppresses plant defense responses during the
CC       early stages of pathogen infection. Suppresses cell death induced by
CC       effectors and PAMPs in plant hosts (PubMed:21653195, PubMed:27256489).
CC       Avh262 stabilizes endoplasmic reticulum (ER)-luminal binding
CC       immunoglobulin proteins (BiPs), which act as negative regulators of
CC       plant resistance to Phytophthora. By stabilizing BiPs, Avh262
CC       suppresses ER stress-triggered cell death and facilitates Phytophthora
CC       infection (PubMed:27256489). {ECO:0000269|PubMed:21653195,
CC       ECO:0000269|PubMed:27256489}.
CC   -!- SUBUNIT: Interacts with host plant ER-luminal binding immunoglobulin
CC       proteins (BiPs) such as soybean BiP1, BiP2, BiP3 and BiP4.
CC       {ECO:0000269|PubMed:27256489}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27256489}. Host
CC       endoplasmic reticulum {ECO:0000269|PubMed:27256489}.
CC   -!- DOMAIN: Residues 60 to 82 are required for the interaction with host
CC       plant binding immunoglobulin proteins (BiPs) and subsequent virulence.
CC       {ECO:0000269|PubMed:27256489}.
CC   -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC       cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC       phosphate. {ECO:0000305|PubMed:21653195}.
CC   -!- DISRUPTION PHENOTYPE: Leads to reduced virulence on etiolated soybean
CC       seedlings. {ECO:0000269|PubMed:27256489}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; JN254251; AEK81064.1; -; Genomic_DNA.
DR   EMBL; JN254252; AEK81065.1; -; Genomic_DNA.
DR   EMBL; JN254253; AEK81066.1; -; Genomic_DNA.
DR   RefSeq; XP_009535769.1; XM_009537474.1.
DR   AlphaFoldDB; E0W5H5; -.
DR   SMR; E0W5H5; -.
DR   GeneID; 20640676; -.
DR   KEGG; psoj:PHYSODRAFT_288708; -.
DR   VEuPathDB; FungiDB:PHYSODRAFT_288708; -.
DR   HOGENOM; CLU_1963950_0_0_1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0044165; C:host cell endoplasmic reticulum; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Host endoplasmic reticulum; Secreted; Signal; Virulence.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..123
FT                   /note="RxLR effector protein Avh262"
FT                   /id="PRO_5007652769"
FT   REGION          24..46
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          60..82
FT                   /note="BiP-binding"
FT                   /evidence="ECO:0000269|PubMed:27256489"
FT   MOTIF           30..50
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:21653195"
FT   COMPBIAS        30..46
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   123 AA;  13259 MW;  394E442DCFCE6DCA CRC64;
     MLPVAVVLVV FAVAVTSAES IHQVNPLPRR RRLKGTEEKG HHTNVNDEER VISLESASDL
     ISKLKVKINA KLLAGDSAKP ATLSKAQVAS VAKEVVKEVK KTPKVWPPPM IKKGVRRGAG
     GVR