ID   AV2_TYLCI               Reviewed;         116 AA.
AC   P27269;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   12-AUG-2020, entry version 61.
DE   RecName: Full=Protein V2;
GN   ORFNames=V2;
OS   Tomato yellow leaf curl virus (strain Israel) (TYLCV).
OC   Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC   Geplafuvirales; Geminiviridae; Begomovirus.
OX   NCBI_TaxID=66366;
OH   NCBI_TaxID=185024; Cynanchum acutum.
OH   NCBI_TaxID=145753; Malva parviflora (Little mallow) (Cheeseweed mallow).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1926771; DOI=10.1016/0042-6822(91)90763-2;
RA   Navot N., Pichersky E., Zeidan M., Zamir D., Czosnek H.;
RT   "Tomato yellow leaf curl virus: a whitefly-transmitted geminivirus with a
RT   single genomic component.";
RL   Virology 185:151-161(1991).
RN   [2]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=Isolate Dominican Republic;
RX   PubMed=11878881; DOI=10.1006/viro.2001.1194;
RA   Rojas M.R., Jiang H., Salati R., Xoconostle-Cazares B., Sudarshana M.R.,
RA   Lucas W.J., Gilbertson R.L.;
RT   "Functional analysis of proteins involved in movement of the monopartite
RT   begomovirus, Tomato yellow leaf curl virus.";
RL   Virology 291:110-125(2001).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF CYS-84 AND CYS-86.
RX   PubMed=16979684; DOI=10.1016/j.virol.2006.08.016;
RA   Zrachya A., Glick E., Levy Y., Arazi T., Citovsky V., Gafni Y.;
RT   "Suppressor of RNA silencing encoded by Tomato yellow leaf curl virus-
RT   Israel.";
RL   Virology 358:159-165(2007).
RN   [4]
RP   FUNCTION, INTERACTION WITH SOLANUM LYCOPERSICUM SGS3, AND MUTAGENESIS OF
RP   CYS-84 AND CYS-86.
RX   PubMed=18165314; DOI=10.1073/pnas.0709036105;
RA   Glick E., Zrachya A., Levy Y., Mett A., Gidoni D., Belausov E.,
RA   Citovsky V., Gafni Y.;
RT   "Interaction with host SGS3 is required for suppression of RNA silencing by
RT   tomato yellow leaf curl virus V2 protein.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:157-161(2008).
CC   -!- FUNCTION: Through its interaction with host SGS3, acts as a suppressor
CC       of RNA-mediated gene silencing, also known as post-transcriptional gene
CC       silencing (PTGS), a mechanism of plant viral defense that limits the
CC       accumulation of viral RNAs. {ECO:0000269|PubMed:16979684,
CC       ECO:0000269|PubMed:18165314}.
CC   -!- SUBUNIT: Interacts with host SGS3. {ECO:0000269|PubMed:18165314}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm, host perinuclear region
CC       {ECO:0000269|PubMed:11878881, ECO:0000269|PubMed:16979684}.
CC       Note=Accumulates in inclusion bodies in the cell periphery. May
CC       interact with the ER network from the perinuclear region out to the
CC       cell periphery.
CC   -!- SIMILARITY: Belongs to the geminiviridae protein AV2/V2 family.
CC       {ECO:0000305}.
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DR   EMBL; X15656; CAA33687.1; -; Genomic_DNA.
DR   PIR; E40779; QQCVB1.
DR   RefSeq; NP_658991.1; NC_004005.1.
DR   GeneID; 949136; -.
DR   KEGG; vg:949136; -.
DR   Proteomes; UP000007547; Genome.
DR   GO; GO:0044220; C:host cell perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019048; P:modulation by virus of host process; IEA:InterPro.
DR   GO; GO:0060967; P:negative regulation of gene silencing by RNA; IEA:InterPro.
DR   InterPro; IPR002511; Gemini_V2.
DR   InterPro; IPR005159; WCCH.
DR   Pfam; PF01524; Gemini_V2; 1.
DR   Pfam; PF03716; WCCH; 1.
PE   1: Evidence at protein level;
KW   Host cytoplasm; Host-virus interaction; Reference proteome;
KW   Suppressor of RNA silencing.
FT   CHAIN           1..116
FT                   /note="Protein V2"
FT                   /id="PRO_0000222277"
FT   MUTAGEN         84
FT                   /note="C->S: Complete loss of suppression of silencing and
FT                   interaction with Solanum lycopersicum SGS3; when associated
FT                   with S-86."
FT                   /evidence="ECO:0000269|PubMed:16979684,
FT                   ECO:0000269|PubMed:18165314"
FT   MUTAGEN         86
FT                   /note="C->S: Complete loss of suppression of silencing and
FT                   interaction with Solanum lycopersicum SGS3; when associated
FT                   with S-84."
FT                   /evidence="ECO:0000269|PubMed:16979684,
FT                   ECO:0000269|PubMed:18165314"
SQ   SEQUENCE   116 AA;  13459 MW;  D506FB53AAF534BB CRC64;
     MWDPLLNEFP ESVHGFRCML AIKYLQSVEE TYEPNTLGHD LIRDLISVVR ARDYVEATRR
     YNHFHARLEG SPKAELRQPI QQPCCCPHCP RHKQATIMDV QAHVPKAQNI QNVSKP