AV331_PHYSP
ID AV331_PHYSP Reviewed; 279 AA.
AC G4ZRQ7; B2C6F5;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 1.
DT 25-MAY-2022, entry version 21.
DE RecName: Full=RxLR effector protein Avh331 {ECO:0000303|PubMed:18390593};
DE AltName: Full=Avirulence homolog protein 1k {ECO:0000303|PubMed:23530601};
DE AltName: Full=Avirulence homolog protein 331 {ECO:0000303|PubMed:18390593};
DE Flags: Precursor;
GN Name=Avh331 {ECO:0000303|PubMed:18390593};
GN Synonyms=Avr1k {ECO:0000303|PubMed:23530601}; ORFNames=PHYSODRAFT_286703;
OS Phytophthora sojae (strain P6497) (Soybean stem and root rot agent)
OS (Phytophthora megasperma f. sp. glycines).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=1094619;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], DOMAIN, AND FUNCTION.
RX PubMed=18390593; DOI=10.1105/tpc.107.057067;
RA Dou D., Kale S.D., Wang X., Chen Y., Wang Q., Wang X., Jiang R.H.,
RA Arredondo F.D., Anderson R.G., Thakur P.B., McDowell J.M., Wang Y.,
RA Tyler B.M.;
RT "Conserved C-terminal motifs required for avirulence and suppression of
RT cell death by Phytophthora sojae effector Avr1b.";
RL Plant Cell 20:1118-1133(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=P6497, P6954, and Race11;
RX PubMed=23530601; DOI=10.1094/mpmi-12-12-0289-r;
RA Song T., Kale S.D., Arredondo F.D., Shen D., Su L., Liu L., Wu Y., Wang Y.,
RA Dou D., Tyler B.M.;
RT "Two RxLR avirulence genes in Phytophthora sojae determine soybean Rps1k-
RT mediated disease resistance.";
RL Mol. Plant Microbe Interact. 26:711-720(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=P6497;
RX PubMed=16946064; DOI=10.1126/science.1128796;
RA Tyler B.M., Tripathy S., Zhang X., Dehal P., Jiang R.H.Y., Aerts A.,
RA Arredondo F.D., Baxter L., Bensasson D., Beynon J.L., Chapman J.,
RA Damasceno C.M.B., Dorrance A.E., Dou D., Dickerman A.W., Dubchak I.L.,
RA Garbelotto M., Gijzen M., Gordon S.G., Govers F., Grunwald N.J., Huang W.,
RA Ivors K.L., Jones R.W., Kamoun S., Krampis K., Lamour K.H., Lee M.-K.,
RA McDonald W.H., Medina M., Meijer H.J.G., Nordberg E.K., Maclean D.J.,
RA Ospina-Giraldo M.D., Morris P.F., Phuntumart V., Putnam N.H., Rash S.,
RA Rose J.K.C., Sakihama Y., Salamov A.A., Savidor A., Scheuring C.F.,
RA Smith B.M., Sobral B.W.S., Terry A., Torto-Alalibo T.A., Win J., Xu Z.,
RA Zhang H., Grigoriev I.V., Rokhsar D.S., Boore J.L.;
RT "Phytophthora genome sequences uncover evolutionary origins and mechanisms
RT of pathogenesis.";
RL Science 313:1261-1266(2006).
RN [4]
RP FUNCTION.
RX DOI=10.1016/j.pmpp.2011.10.002;
RA Cheng B., Yu X., Ma Z., Dong S., Dou D., Wang Y., Zheng X.;
RT "Phytophthora sojae effector Avh331 suppresses the plant defence responseby
RT disturbing the MAPK signalling pathway.";
RL Physiol. Mol. Plant Pathol. 77:1-9(2012).
CC -!- FUNCTION: Effector that suppresses the host mitogen-activated protein
CC kinase (MAPK)-based plant defense activated by the Phytophthora
CC elicitor to promote colonization of the Phytophthora pathogen
CC (PubMed:18390593, PubMed:23530601, Ref.4). Neither directly inhibits
CC MAPK kinase activity nor interacts with MAPK proteins but acts
CC downstream by suppressing transcriptional activation of resistance
CC marker genes such as FRK1, WRKY22 and WRKY29 (Ref.4). Confers
CC avirulence in the presence of resistance protein Rps1k in host
CC (PubMed:23530601). {ECO:0000269|PubMed:18390593,
CC ECO:0000269|PubMed:23530601, ECO:0000269|Ref.4}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|Ref.4}. Host cell
CC {ECO:0000305|Ref.4}.
CC -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC phosphate. {ECO:0000305|PubMed:18390593}.
CC -!- DOMAIN: The C-terminal region contains 1 L, 2 W and 2 Y motifs. W, Y
CC and L motifs are present in at least half of the identified oomycete
CC RXLR-dEER effector candidates and W and Y are critical for the
CC functions of the very large number of predicted oomycete effectors that
CC contain them. {ECO:0000305|PubMed:18390593}.
CC -!- DISRUPTION PHENOTYPE: Results in loss of the avirulence phenotype on
CC soybean cultivar containing the resistance protein Rps1k.
CC {ECO:0000269|PubMed:23530601}.
CC -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR EMBL; KC312954; AGC95073.1; -; Genomic_DNA.
DR EMBL; KC312952; AGC95072.1; -; Genomic_DNA.
DR EMBL; KC312951; AGC95071.1; -; Genomic_DNA.
DR EMBL; EU282487; ABZ10806.1; -; Genomic_DNA.
DR EMBL; JH159156; EGZ13866.1; -; Genomic_DNA.
DR RefSeq; XP_009531295.1; XM_009533000.1.
DR AlphaFoldDB; G4ZRQ7; -.
DR SMR; G4ZRQ7; -.
DR EnsemblProtists; EGZ13866; EGZ13866; PHYSODRAFT_286703.
DR GeneID; 20640446; -.
DR KEGG; psoj:PHYSODRAFT_286703; -.
DR PHI-base; PHI:2890; -.
DR Proteomes; UP000002640; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0043657; C:host cell; IEA:UniProtKB-SubCell.
PE 3: Inferred from homology;
KW Glycoprotein; Reference proteome; Repeat; Secreted; Signal; Virulence.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..279
FT /note="RxLR effector protein Avh331"
FT /id="PRO_0000448091"
FT REGION 129..147
FT /note="W1 motif"
FT /evidence="ECO:0000305|PubMed:18390593"
FT REGION 153..174
FT /note="Y1 motif"
FT /evidence="ECO:0000305|PubMed:18390593"
FT REGION 178..208
FT /note="L motif"
FT /evidence="ECO:0000305|PubMed:18390593"
FT REGION 222..240
FT /note="W2 motif"
FT /evidence="ECO:0000305|PubMed:18390593"
FT REGION 250..271
FT /note="Y2 motif"
FT /evidence="ECO:0000305|PubMed:18390593"
FT MOTIF 86..106
FT /note="RxLR-dEER"
FT /evidence="ECO:0000305|PubMed:18390593"
FT CARBOHYD 100
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 279 AA; 30894 MW; 0B2445D65D02BCE2 CRC64;
MMQWSAILIR TCFSGSGGEA LTCATSEQQT RPELCFFFSV RSSWPSTISD GACLALVSAE
QGATAGRNTL SLRSMMATED MATSTRSLRS QATNVDDDAN VSIENRGMNP SVLTKLGEFA
STLTAGNTAN KLWLMADVDP KSAFKLLGLD MPGVRFIDNP KMLQWLKFTK AYLDMKKSGF
GETSAHALLY EKIGGPDLSL LLLSLKDAPD ANSLVQKLTN SQFGMWHDAR IEPEQLAQTV
FKIQDVRKLP KNDPKLQVID DYAKYHRKHR KFLNSIMII
