ID   AVEE_AVESA              Reviewed;         182 AA.
AC   Q09114; Q09096;
DT   13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Avenin-E;
DE   AltName: Full=Alpha-2 avenin;
DE   AltName: Full=Avenin N9;
DE   AltName: Full=Celiac immunoreactive protein 3;
DE            Short=CIP-3;
DE   AltName: Full=Prolamin;
OS   Avena sativa (Oat).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Poodae; Poeae; Aveninae; Avena.
OX   NCBI_TaxID=4498;
RN   [1] {ECO:0000312|PIR:JG0015}
RP   PROTEIN SEQUENCE.
RX   PubMed=7165690; DOI=10.1007/bf00498931;
RA   Bietz J.A.;
RT   "Cereal prolamin evolution and homology revealed by sequence analysis.";
RL   Biochem. Genet. 20:1039-1053(1982).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE.
RC   STRAIN=cv. Narymsky 943 {ECO:0000269|PubMed:3190779};
RX   PubMed=3190779;
RA   Egorov T.A.;
RT   "Primary structure of the 'fast' component of avenin (Avena sativa L.";
RL   Bioorg. Khim. 14:959-962(1988).
RN   [3] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 1-51.
RC   STRAIN=cv. Rhea {ECO:0000269|PubMed:3120802};
RC   TISSUE=Seed {ECO:0000269|PubMed:3120802};
RX   PubMed=3120802; DOI=10.1016/0300-9084(87)90189-1;
RA   Pernollet J.-C., Huet J.-C., Galle A.-M., Sallantin M.;
RT   "N-terminal sequences of oat avenins compared to other cereal prolamins.";
RL   Biochimie 69:683-689(1987).
RN   [4] {ECO:0000305, ECO:0000312|PIR:S29209}
RP   PROTEIN SEQUENCE OF 1-14.
RC   TISSUE=Endosperm {ECO:0000269|PubMed:1526282};
RX   PubMed=1526282; DOI=10.1016/0014-5793(92)81141-8;
RA   Rocher A., Colilla F., Ortiz M.L., Mendez E.;
RT   "Identification of the three major coeliac immunoreactive proteins and one
RT   alpha-amylase inhibitor from oat endosperm.";
RL   FEBS Lett. 310:37-40(1992).
CC   -!- FUNCTION: Seed storage protein. Serves as a source of nitrogen, carbon,
CC       and sulfur for the young developing seedling (By similarity).
CC       {ECO:0000250|UniProtKB:P80356}.
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:P80356}.
CC   -!- SUBCELLULAR LOCATION: Vacuole, aleurone grain {ECO:0000250}.
CC       Note=Protein bodies inside vacuoles. {ECO:0000250}.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Avenins are one of the
CC       causes of celiac disease, also known as celiac sprue or gluten-
CC       sensitive enteropathy (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the gliadin/glutenin family. {ECO:0000255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   PIR; JG0015; JG0015.
DR   PIR; S29209; S29209.
DR   AlphaFoldDB; Q09114; -.
DR   GO; GO:0033095; C:aleurone grain; IEA:UniProtKB-SubCell.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-KW.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   CDD; cd00261; AAI_SS; 1.
DR   Gene3D; 1.10.110.10; -; 1.
DR   InterPro; IPR044723; AAI_SS_dom.
DR   InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR   InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR   InterPro; IPR001954; Glia_glutenin.
DR   PANTHER; PTHR33454; PTHR33454; 1.
DR   Pfam; PF13016; Gliadin; 1.
DR   PRINTS; PR00208; GLIADGLUTEN.
DR   SMART; SM00499; AAI; 1.
DR   SUPFAM; SSF47699; SSF47699; 1.
PE   1: Evidence at protein level;
KW   Allergen; Direct protein sequencing; Repeat; Seed storage protein;
KW   Storage protein; Vacuole.
FT   CHAIN           1..182
FT                   /note="Avenin-E"
FT                   /id="PRO_0000239309"
FT   REPEAT          21..26
FT                   /note="1; approximate"
FT   REPEAT          27..34
FT                   /note="2; approximate"
FT   REPEAT          35..41
FT                   /note="3"
FT                   /evidence="ECO:0000255"
FT   REGION          21..41
FT                   /note="3 X 7 AA tandem repeats of P-F-V-Q-Q-Q-Q"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        45
FT                   /note="Q -> QE (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   182 AA;  21036 MW;  F0625FCE6383D2DC CRC64;
     TTTVQYNPSE QYQPYPEQQE PFVQQQPFVQ QQQQPFVQQQ QMFLQPLLQQ QLNPCKQFLV
     QQCSPVAVVP FLRSQILRQA ICQVARQQCC RQLAQIPEQL RCPAIHSVVQ AIILQQQQQQ
     QFFQPQLQQQ VFQPQLQQVF NQPQQQAQFE GMRAFALQAL PAMCDVYVPP QCPVATAPLG
     GF