位置:首页 > 蛋白库 > AVFA_DOTSN
AVFA_DOTSN
ID   AVFA_DOTSN              Reviewed;         284 AA.
AC   M2XHE6;
DT   28-FEB-2018, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2013, sequence version 1.
DT   03-AUG-2022, entry version 26.
DE   RecName: Full=Averufin oxidase A {ECO:0000250|UniProtKB:Q12437};
DE            EC=1.-.-.- {ECO:0000250|UniProtKB:Q12437};
DE   AltName: Full=Dothistromin biosynthesis protein avfA {ECO:0000303|PubMed:23207690};
DE   Flags: Precursor;
GN   Name=avfA {ECO:0000303|PubMed:23207690}; ORFNames=DOTSEDRAFT_75546;
OS   Dothistroma septosporum (strain NZE10 / CBS 128990) (Red band needle blight
OS   fungus) (Mycosphaerella pini).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Dothistroma.
OX   NCBI_TaxID=675120;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NZE10 / CBS 128990;
RX   PubMed=23209441; DOI=10.1371/journal.pgen.1003088;
RA   de Wit P.J.G.M., van der Burgt A., Oekmen B., Stergiopoulos I.,
RA   Abd-Elsalam K.A., Aerts A.L., Bahkali A.H., Beenen H.G., Chettri P.,
RA   Cox M.P., Datema E., de Vries R.P., Dhillon B., Ganley A.R.,
RA   Griffiths S.A., Guo Y., Hamelin R.C., Henrissat B., Kabir M.S.,
RA   Jashni M.K., Kema G., Klaubauf S., Lapidus A., Levasseur A., Lindquist E.,
RA   Mehrabi R., Ohm R.A., Owen T.J., Salamov A., Schwelm A., Schijlen E.,
RA   Sun H., van den Burg H.A., van Ham R.C.H.J., Zhang S., Goodwin S.B.,
RA   Grigoriev I.V., Collemare J., Bradshaw R.E.;
RT   "The genomes of the fungal plant pathogens Cladosporium fulvum and
RT   Dothistroma septosporum reveal adaptation to different hosts and lifestyles
RT   but also signatures of common ancestry.";
RL   PLoS Genet. 8:E1003088-E1003088(2012).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NZE10 / CBS 128990;
RX   PubMed=23236275; DOI=10.1371/journal.ppat.1003037;
RA   Ohm R.A., Feau N., Henrissat B., Schoch C.L., Horwitz B.A., Barry K.W.,
RA   Condon B.J., Copeland A.C., Dhillon B., Glaser F., Hesse C.N., Kosti I.,
RA   LaButti K., Lindquist E.A., Lucas S., Salamov A.A., Bradshaw R.E.,
RA   Ciuffetti L., Hamelin R.C., Kema G.H.J., Lawrence C., Scott J.A.,
RA   Spatafora J.W., Turgeon B.G., de Wit P.J.G.M., Zhong S., Goodwin S.B.,
RA   Grigoriev I.V.;
RT   "Diverse lifestyles and strategies of plant pathogenesis encoded in the
RT   genomes of eighteen Dothideomycetes fungi.";
RL   PLoS Pathog. 8:E1003037-E1003037(2012).
RN   [3]
RP   FUNCTION.
RX   PubMed=12039746; DOI=10.1128/aem.68.6.2885-2892.2002;
RA   Bradshaw R.E., Bhatnagar D., Ganley R.J., Gillman C.J., Monahan B.J.,
RA   Seconi J.M.;
RT   "Dothistroma pini, a forest pathogen, contains homologs of aflatoxin
RT   biosynthetic pathway genes.";
RL   Appl. Environ. Microbiol. 68:2885-2892(2002).
RN   [4]
RP   FUNCTION.
RX   PubMed=16649078; DOI=10.1007/s11046-006-0240-5;
RA   Bradshaw R.E., Jin H., Morgan B.S., Schwelm A., Teddy O.R., Young C.A.,
RA   Zhang S.;
RT   "A polyketide synthase gene required for biosynthesis of the aflatoxin-like
RT   toxin, dothistromin.";
RL   Mycopathologia 161:283-294(2006).
RN   [5]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=17683963; DOI=10.1016/j.fgb.2007.06.005;
RA   Zhang S., Schwelm A., Jin H., Collins L.J., Bradshaw R.E.;
RT   "A fragmented aflatoxin-like gene cluster in the forest pathogen
RT   Dothistroma septosporum.";
RL   Fungal Genet. Biol. 44:1342-1354(2007).
RN   [6]
RP   REVIEW ON FUNCTION, AND PATHWAY.
RX   PubMed=22069571; DOI=10.3390/toxins2112680;
RA   Schwelm A., Bradshaw R.E.;
RT   "Genetics of dothistromin biosynthesis of Dothistroma septosporum: an
RT   update.";
RL   Toxins 2:2680-2698(2010).
RN   [7]
RP   FUNCTION, INDUCTION, AND PATHWAY.
RX   PubMed=23207690; DOI=10.1016/j.fgb.2012.11.006;
RA   Chettri P., Ehrlich K.C., Cary J.W., Collemare J., Cox M.P.,
RA   Griffiths S.A., Olson M.A., de Wit P.J., Bradshaw R.E.;
RT   "Dothistromin genes at multiple separate loci are regulated by AflR.";
RL   Fungal Genet. Biol. 51:12-20(2013).
RN   [8]
RP   FUNCTION.
RX   PubMed=23448391; DOI=10.1111/nph.12161;
RA   Bradshaw R.E., Slot J.C., Moore G.G., Chettri P., de Wit P.J.,
RA   Ehrlich K.C., Ganley A.R., Olson M.A., Rokas A., Carbone I., Cox M.P.;
RT   "Fragmentation of an aflatoxin-like gene cluster in a forest pathogen.";
RL   New Phytol. 198:525-535(2013).
CC   -!- FUNCTION: Averufin oxidase A; part of the fragmented gene cluster that
CC       mediates the biosynthesis of dothistromin (DOTH), a polyketide toxin
CC       very similar in structure to the aflatoxin precursor, versicolorin B
CC       (PubMed:12039746, PubMed:17683963, PubMed:22069571, PubMed:23207690,
CC       PubMed:23448391). The first step of the pathway is the conversion of
CC       acetate to norsolorinic acid (NOR) and requires the fatty acid synthase
CC       subunits hexA and hexB, as well as the polyketide synthase pksA
CC       (PubMed:16649078, PubMed:23207690). PksA combines a hexanoyl starter
CC       unit and 7 malonyl-CoA extender units to synthesize the precursor NOR
CC       (By similarity). The hexanoyl starter unit is provided to the acyl-
CC       carrier protein (ACP) domain by the fungal fatty acid synthase
CC       hexA/hexB (By similarity). The second step is the conversion of NOR to
CC       averantin (AVN) and requires the norsolorinic acid ketoreductase nor1,
CC       which catalyzes the dehydration of norsolorinic acid to form (1'S)-
CC       averantin (PubMed:23207690). The cytochrome P450 monooxygenase avnA
CC       then catalyzes the hydroxylation of AVN to 5'hydroxyaverantin (HAVN)
CC       (PubMed:23207690). The next step is performed by adhA that transforms
CC       HAVN to averufin (AVF) (PubMed:23207690). Averufin might then be
CC       converted to hydroxyversicolorone by cypX and avfA (PubMed:23207690).
CC       Hydroxyversicolorone is further converted versiconal hemiacetal acetate
CC       (VHA) by moxY (PubMed:23207690). VHA is then the substrate for the
CC       versiconal hemiacetal acetate esterase est1 to yield versiconal (VAL)
CC       (PubMed:23207690). Versicolorin B synthase vbsA then converts VAL to
CC       versicolorin B (VERB) by closing the bisfuran ring (PubMed:16649078,
CC       PubMed:23207690). Then, the activity of the versicolorin B desaturase
CC       verB leads to versicolorin A (VERA) (PubMed:23207690). DotB, a
CC       predicted chloroperoxidase, may perform epoxidation of the A-ring of
CC       VERA (PubMed:23207690). Alternatively, a cytochrome P450, such as cypX
CC       or avnA could catalyze this step (PubMed:23207690). It is also possible
CC       that another, uncharacterized, cytochrome P450 enzyme is responsible
CC       for this step (PubMed:23207690). Opening of the epoxide could
CC       potentially be achieved by the epoxide hydrolase epoA
CC       (PubMed:23207690). However, epoA seems not to be required for DOTH
CC       biosynthesis, but other epoxide hydrolases may have the ability to
CC       complement this hydrolysis (PubMed:23207690). Alternatively, opening of
CC       the epoxide ring could be achieved non-enzymatically (PubMed:23207690).
CC       The next step is the deoxygenation of ring A to yield the 5,8-
CC       dihydroxyanthraquinone which is most likely catalyzed by the NADPH
CC       dehydrogenase encoded by ver1 (PubMed:23207690). The last stages of
CC       DOTH biosynthesis are proposed to involve hydroxylation of the bisfuran
CC       (PubMed:23207690). OrdB and norB might have oxidative roles here
CC       (PubMed:23207690). An alternative possibility is that cytochrome P450
CC       monoogenases such as avnA and cypX might perform these steps in
CC       addition to previously proposed steps (PubMed:23207690).
CC       {ECO:0000250|UniProtKB:Q12437, ECO:0000269|PubMed:12039746,
CC       ECO:0000269|PubMed:16649078, ECO:0000303|PubMed:22069571,
CC       ECO:0000305|PubMed:17683963, ECO:0000305|PubMed:23207690,
CC       ECO:0000305|PubMed:23448391}.
CC   -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000303|PubMed:22069571,
CC       ECO:0000305|PubMed:23207690}.
CC   -!- INDUCTION: Expression is positively regulated by the dothistromin-
CC       specific transcription factor aflR (PubMed:23207690). Dothistromin
CC       biosynthetic proteins are co-regulated, showing a high level of
CC       expression at ealy exponential phase with a subsequent decline in older
CC       cultures (PubMed:17683963). {ECO:0000269|PubMed:17683963,
CC       ECO:0000269|PubMed:23207690}.
CC   -!- SIMILARITY: Belongs to the avfA family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; KB446546; EME38862.1; -; Genomic_DNA.
DR   AlphaFoldDB; M2XHE6; -.
DR   EnsemblFungi; EME38862; EME38862; DOTSEDRAFT_75546.
DR   eggNOG; ENOG502SM0C; Eukaryota.
DR   HOGENOM; CLU_090039_0_0_1; -.
DR   OMA; GAAMCEI; -.
DR   OrthoDB; 1166292at2759; -.
DR   Proteomes; UP000016933; Unassembled WGS sequence.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0044281; P:small molecule metabolic process; IEA:UniProt.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF13460; NAD_binding_10; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Oxidoreductase; Reference proteome; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..284
FT                   /note="Averufin oxidase A"
FT                   /id="PRO_0000443466"
FT   CARBOHYD        62
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        86
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        190
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   284 AA;  30716 MW;  A3F5B919AFFB7C24 CRC64;
     MPTYALLGAT GATGSAILRC LLASPPPDLD LNILVRSKQK LLKSFPTLTT TISPRIHIIQ
     GNSTDTIALQ QCLEDASVAF MCVADNASNK GVSLTADTVT AIVTTLGMLR KLHGSAYNAP
     TILQLRSASL NPKLSCQVPR LVYNIVSFCL HYSHLDIVKA CEHYEAAAAK GLLSYIYVDP
     PTIHDAFGPN RTGHKLISCK PDVCDKQETA LSYADLGAGF VEIASRKEDF LNQPVGVTAT
     GKAKETWGVL AGFLFDGAKG RARAWWEEER PMSKPQNLFL YCGM
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024