ID   AVH4_PHYMI              Reviewed;         290 AA.
AC   C5IL49;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 17.
DE   RecName: Full=RxLR effector protein Avr4 {ECO:0000303|PubMed:19694952};
DE   AltName: Full=Avirulence homolog protein 4 {ECO:0000303|PubMed:19694952};
DE   Flags: Precursor;
GN   Name=Avh4 {ECO:0000303|PubMed:19694952};
OS   Phytophthora mirabilis.
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=129356;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DOMAIN.
RC   STRAIN=PIC 99111;
RX   PubMed=19694952; DOI=10.1111/j.1364-3703.2009.00556.x;
RA   Van Poppel P.M., Jiang R.H., Sliwka J., Govers F.;
RT   "Recognition of Phytophthora infestans Avr4 by potato R4 is triggered by C-
RT   terminal domains comprising W motifs.";
RL   Mol. Plant Pathol. 10:611-620(2009).
CC   -!- FUNCTION: Secreted effector that acts as an elicitor of hypersensitive
CC       response (HR) specifically on plants carrying defense protein R4,
CC       through its interaction with this protein.
CC       {ECO:0000269|PubMed:19694952}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:B1NNT7}. Host
CC       cytoplasm {ECO:0000250|UniProtKB:B1NNT7}. Host nucleus
CC       {ECO:0000250|UniProtKB:B1NNT7}. Host nucleus, host nucleolus
CC       {ECO:0000250|UniProtKB:B1NNT7}. Host cytoplasm, host cytoskeleton
CC       {ECO:0000250|UniProtKB:B1NNT7}.
CC   -!- DOMAIN: Oomycete Avh family proteins have a modular structure with an
CC       N-terminal signal peptide (SP), an RXLR-dEER domain and a C-terminus
CC       with a variable number of modules that consist of W, Y and L motifs
CC       after highly conserved residues. The C-ter of Avr4 contains 3 W motifs,
CC       1 Y motif but no L motif. The RXLR-dEER motif functions as a host
CC       targeting signal (HTS). The region containing the W2 motif, in
CC       combination with either the W1 or W3 motif, triggers a necrotic
CC       response in host plants carrying the resistance gene R4.
CC       {ECO:0000305|PubMed:19694952}.
CC   -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR   EMBL; FJ951909; ACR10586.1; -; Genomic_DNA.
DR   AlphaFoldDB; C5IL49; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044163; C:host cytoskeleton; IEA:UniProtKB-SubCell.
PE   3: Inferred from homology;
KW   Glycoprotein; Host cytoplasm; Host cytoskeleton; Host nucleus; Secreted;
KW   Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..290
FT                   /note="RxLR effector protein Avr4"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5002953493"
FT   REGION          33..56
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          118..141
FT                   /note="W1 motif"
FT                   /evidence="ECO:0000305|PubMed:19694952"
FT   REGION          151..174
FT                   /note="W2 motif"
FT                   /evidence="ECO:0000305|PubMed:19694952"
FT   REGION          224..247
FT                   /note="W3 motif"
FT                   /evidence="ECO:0000305|PubMed:19694952"
FT   REGION          249..270
FT                   /note="Y motif"
FT                   /evidence="ECO:0000305|PubMed:19694952"
FT   MOTIF           42..58
FT                   /note="RxLR-dEER"
FT                   /evidence="ECO:0000305|PubMed:19694952"
FT   COMPBIAS        35..56
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        246
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   290 AA;  33350 MW;  660A7551BC19306F CRC64;
     MRSLHILLVI TASLLASLAV SAEADPSTRT ANVVENNKDK SRFLRDGGTT EAQTDEERAT
     ITLGDKVVSD KAATKDLLER LLALGTPLKT VQKEFLNMPL IKTFAELSKH PNWRALDKYE
     RMQWQKLNEG QTLTYMRVGD RSYSKEKAQE QLLRWVAQKK TVKSVYDDLQ IEGFARNTDA
     ARLNWRAYNM YDKWFTAASQ MQRNPQQYAK FGTGYHSEQK TTEVFEKWAM EGTHIKSVIK
     TLNLNNKSAS EMANNENFPA LLKYVKLYLD FKPFRDLNAK SRLQARRPIS