AVH52_PHYSP
ID AVH52_PHYSP Reviewed; 122 AA.
AC G4ZSG0;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 25-JAN-2012, sequence version 1.
DT 25-MAY-2022, entry version 26.
DE RecName: Full=RxLR effector protein Avh52 {ECO:0000303|PubMed:21653195};
DE AltName: Full=Avirulence homolog protein 52 {ECO:0000303|PubMed:21653195};
DE Flags: Precursor;
GN Name=Avh52 {ECO:0000303|PubMed:21653195}; ORFNames=PHYSODRAFT_355041;
OS Phytophthora sojae (strain P6497) (Soybean stem and root rot agent)
OS (Phytophthora megasperma f. sp. glycines).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=1094619;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=P6497;
RX PubMed=16946064; DOI=10.1126/science.1128796;
RA Tyler B.M., Tripathy S., Zhang X., Dehal P., Jiang R.H.Y., Aerts A.,
RA Arredondo F.D., Baxter L., Bensasson D., Beynon J.L., Chapman J.,
RA Damasceno C.M.B., Dorrance A.E., Dou D., Dickerman A.W., Dubchak I.L.,
RA Garbelotto M., Gijzen M., Gordon S.G., Govers F., Grunwald N.J., Huang W.,
RA Ivors K.L., Jones R.W., Kamoun S., Krampis K., Lamour K.H., Lee M.-K.,
RA McDonald W.H., Medina M., Meijer H.J.G., Nordberg E.K., Maclean D.J.,
RA Ospina-Giraldo M.D., Morris P.F., Phuntumart V., Putnam N.H., Rash S.,
RA Rose J.K.C., Sakihama Y., Salamov A.A., Savidor A., Scheuring C.F.,
RA Smith B.M., Sobral B.W.S., Terry A., Torto-Alalibo T.A., Win J., Xu Z.,
RA Zhang H., Grigoriev I.V., Rokhsar D.S., Boore J.L.;
RT "Phytophthora genome sequences uncover evolutionary origins and mechanisms
RT of pathogenesis.";
RL Science 313:1261-1266(2006).
RN [2]
RP IDENTIFICATION, FUNCTION, AND DOMAIN.
RX PubMed=21653195; DOI=10.1105/tpc.111.086082;
RA Wang Q., Han C., Ferreira A.O., Yu X., Ye W., Tripathy S., Kale S.D.,
RA Gu B., Sheng Y., Sui Y., Wang X., Zhang Z., Cheng B., Dong S., Shan W.,
RA Zheng X., Dou D., Tyler B.M., Wang Y.;
RT "Transcriptional programming and functional interactions within the
RT Phytophthora sojae RXLR effector repertoire.";
RL Plant Cell 23:2064-2086(2011).
RN [3]
RP FUNCTION.
RX PubMed=26163574; DOI=10.1105/tpc.15.00390;
RA Ma Z., Song T., Zhu L., Ye W., Wang Y., Shao Y., Dong S., Zhang Z., Dou D.,
RA Zheng X., Tyler B.M., Wang Y.;
RT "A Phytophthora sojae glycoside hydrolase 12 protein is a major virulence
RT factor during soybean infection and is recognized as a PAMP.";
RL Plant Cell 27:2057-2072(2015).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH HOST TAP1, SUBCELLULAR
RP LOCATION, DOMAIN, AND MUTAGENESIS OF 69-SER--VAL-86; LYS-87; LYS-88;
RP LYS-91; LYS-95; LYS-97 AND LYS-98.
RX PubMed=30346270; DOI=10.7554/elife.40039;
RA Li H., Wang H., Jing M., Zhu J., Guo B., Wang Y., Lin Y., Chen H., Kong L.,
RA Ma Z., Wang Y., Ye W., Dong S., Tyler B., Wang Y.;
RT "A Phytophthora effector recruits a host cytoplasmic transacetylase into
RT nuclear speckles to enhance plant susceptibility.";
RL Elife 7:0-0(2018).
CC -!- FUNCTION: Effector that suppresses plant defense responses during the
CC early stages of pathogen infection (PubMed:21653195, PubMed:26163574,
CC PubMed:30346270). Suppresses cell death induced by effectors and PAMPs
CC in plant hosts (PubMed:26163574, PubMed:30346270). Interacts with host
CC acetyltransferase TAP1 and causes TAP1 relocation into the nucleus
CC where it acetylates histones H2A and H3 during early infection, thereby
CC promoting susceptibility of host plant to P.sojae (PubMed:30346270).
CC {ECO:0000269|PubMed:21653195, ECO:0000269|PubMed:26163574,
CC ECO:0000269|PubMed:30346270}.
CC -!- SUBUNIT: Interacts with host acetyl transferase TAP1.
CC {ECO:0000269|PubMed:30346270}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30346270}. Host
CC nucleus {ECO:0000269|PubMed:30346270}.
CC -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC phosphate. {ECO:0000305|PubMed:21653195}.
CC -!- DOMAIN: Residues 69 to 86 are required for the interaction with host
CC TAP1 and its re-localization into nuclear speckles.
CC {ECO:0000269|PubMed:30346270}.
CC -!- DOMAIN: The nuclear localization signal (NLS) is required for
CC localization into nucleus. {ECO:0000269|PubMed:30346270}.
CC -!- DISRUPTION PHENOTYPE: Leads to smaller lesions on soybean seedlings.
CC {ECO:0000269|PubMed:30346270}.
CC -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR EMBL; JH159156; EGZ14040.1; -; Genomic_DNA.
DR RefSeq; XP_009531469.1; XM_009533174.1.
DR AlphaFoldDB; G4ZSG0; -.
DR EnsemblProtists; EGZ14040; EGZ14040; PHYSODRAFT_355041.
DR GeneID; 20649783; -.
DR KEGG; psoj:PHYSODRAFT_355041; -.
DR InParanoid; G4ZSG0; -.
DR OMA; LAWAMKE; -.
DR Proteomes; UP000002640; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR InterPro; IPR031825; RXLR.
DR Pfam; PF16810; RXLR; 1.
PE 1: Evidence at protein level;
KW Host nucleus; Reference proteome; Secreted; Signal; Virulence.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..122
FT /note="RxLR effector protein Avh52"
FT /id="PRO_5003472523"
FT REGION 69..86
FT /note="TAP1-binding"
FT /evidence="ECO:0000269|PubMed:30346270"
FT REGION 87..98
FT /note="Nuclear localization signal (NLS)"
FT /evidence="ECO:0000269|PubMed:30346270"
FT MOTIF 50..68
FT /note="RxLR-dEER"
FT /evidence="ECO:0000305|PubMed:30346270"
FT MUTAGEN 69..86
FT /note="Missing: Impairs the interaction with host TAP1 and
FT does not cause re-localization of TAP1 into nuclear
FT speckles."
FT /evidence="ECO:0000269|PubMed:30346270"
FT MUTAGEN 87
FT /note="K->A: Impairs host nuclear localization; when
FT associated with A-88, A-91, A-95, A-97 and A-98."
FT /evidence="ECO:0000269|PubMed:30346270"
FT MUTAGEN 88
FT /note="K->A: Impairs host nuclear localization; when
FT associated with A-87, A-91, A-95, A-97 and A-98."
FT /evidence="ECO:0000269|PubMed:30346270"
FT MUTAGEN 91
FT /note="K->A: Impairs host nuclear localization; when
FT associated with A-87, A-88, A-95, A-97 and A-98."
FT /evidence="ECO:0000269|PubMed:30346270"
FT MUTAGEN 95
FT /note="K->A: Impairs host nuclear localization; when
FT associated with A-87, A-88, A-91, A-97 and A-98."
FT /evidence="ECO:0000269|PubMed:30346270"
FT MUTAGEN 97
FT /note="K->A: Impairs host nuclear localization; when
FT associated with A-87, A-88, A-91, A-95 and A-98."
FT /evidence="ECO:0000269|PubMed:30346270"
FT MUTAGEN 98
FT /note="K->A: Impairs host nuclear localization; when
FT associated with A-87, A-88, A-91, A-95 and A-97."
FT /evidence="ECO:0000269|PubMed:30346270"
SQ SEQUENCE 122 AA; 13484 MW; C58D461962991D83 CRC64;
MRLTSILVLV IAATFHTTGT ALTLTKDSKA GIANGDSPAS GDFIDANSAR LLRRVEKDKV
DYEQDEQRSF GALKDAVKKL NPVTAVKKFF KQRAKRKKVI QTARDADNNL AWAMKEVYKA
AN
