AVL9_YEAST
ID AVL9_YEAST Reviewed; 764 AA.
AC Q12500; D6VYB2;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Late secretory pathway protein AVL9;
DE AltName: Full=APL2 VPS1 synthetic lethal protein 9;
GN Name=AVL9; OrderedLocusNames=YLR114C; ORFNames=L2941;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 90840 / EAY235 / FY23;
RX PubMed=9090053;
RX DOI=10.1002/(sici)1097-0061(19970315)13:3<241::aid-yea61>3.0.co;2-#;
RA Verhasselt P., Volckaert G.;
RT "Sequence analysis of a 37.6 kbp cosmid clone from the right arm of
RT Saccharomyces cerevisiae chromosome XII, carrying YAP3, HOG1, SNR6, tRNA-
RT Arg3 and 23 new open reading frames, among which several homologies to
RT proteins involved in cell division control and to mammalian growth factors
RT and other animal proteins are found.";
RL Yeast 13:241-250(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP FUNCTION.
RX PubMed=17229886; DOI=10.1091/mbc.e06-11-1035;
RA Harsay E., Schekman R.;
RT "Avl9p, a member of a novel protein superfamily, functions in the late
RT secretory pathway.";
RL Mol. Biol. Cell 18:1203-1219(2007).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-524, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-519, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Functions in the late secretory pathway. Required for the
CC generation of secretory vesicles as well as for actin polarization and
CC polarized growth. {ECO:0000269|PubMed:17229886}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 7500 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the AVL9 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X89514; CAA61692.1; -; Genomic_DNA.
DR EMBL; U53878; AAB67559.1; -; Genomic_DNA.
DR EMBL; Z73286; CAA97681.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09428.1; -; Genomic_DNA.
DR PIR; S64951; S64951.
DR RefSeq; NP_013215.1; NM_001182001.1.
DR AlphaFoldDB; Q12500; -.
DR BioGRID; 31386; 260.
DR DIP; DIP-4819N; -.
DR IntAct; Q12500; 1.
DR STRING; 4932.YLR114C; -.
DR CarbonylDB; Q12500; -.
DR iPTMnet; Q12500; -.
DR MaxQB; Q12500; -.
DR PaxDb; Q12500; -.
DR PRIDE; Q12500; -.
DR EnsemblFungi; YLR114C_mRNA; YLR114C; YLR114C.
DR GeneID; 850805; -.
DR KEGG; sce:YLR114C; -.
DR SGD; S000004104; AVL9.
DR VEuPathDB; FungiDB:YLR114C; -.
DR eggNOG; KOG3823; Eukaryota.
DR GeneTree; ENSGT00390000010255; -.
DR HOGENOM; CLU_009066_3_0_1; -.
DR InParanoid; Q12500; -.
DR OMA; TWNENDE; -.
DR BioCyc; YEAST:G3O-32259-MON; -.
DR PRO; PR:Q12500; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q12500; protein.
DR GO; GO:0005935; C:cellular bud neck; HDA:SGD.
DR GO; GO:0005934; C:cellular bud tip; HDA:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0006892; P:post-Golgi vesicle-mediated transport; IMP:SGD.
DR InterPro; IPR018307; ABL9/DENND6_dom.
DR InterPro; IPR037516; Tripartite_DENN.
DR Pfam; PF09794; Avl9; 1.
DR PROSITE; PS50211; DENN; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..764
FT /note="Late secretory pathway protein AVL9"
FT /id="PRO_0000247424"
FT DOMAIN 10..173
FT /note="uDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 195..360
FT /note="cDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 362..464
FT /note="dDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT REGION 490..535
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 550..572
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 587..764
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 502..516
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 517..535
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 595..661
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 698..744
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 519
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 524
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
SQ SEQUENCE 764 AA; 86426 MW; 159A00CF933A5C4A CRC64;
MDEHEEAVIF GICLVDFHHK RGPEIEYWYG LPEGTQSAEL WPNLPFQALP DGSHSFEETF
TYFTLLYDER RQRSPPNGAT DLSDDSINDN TTLFAISCSR QIKSDELVTK DKDVTRSTVQ
KAIVVISRQP IFGQIKDKLS IVTNAFFLQH DFGDRKIIQS LYENLKSIYT PASLVRNAEN
RLYIGLCLRK ILHDFKRNAL VLLKAIMLEK KIIVYGNDVE ALCNLQFGLI SLIPDLMSNL
QDSGSPQLFQ DISKLNVVDS FKSSNRESVL RFLGFPLPIF EKGGLFSPYT PLQQMNDIRS
ERTLFFMIGS SNTLLAEQKE ELCHIFVNTD NSTVDILDKT LNPVLQLSSH DKKWIESISG
IVSDTWNEND DETPKNSQFE GSEDFIRWQF EDYLTGLLSS VKLSDYLDLH KENDQALKTI
PEDMLNSNPV HLFNLNWVQS WKETQNFLIF NSRTDDRLFD LFPPKHIYNG ADTLSLLQQR
FLATFHNLKR SSSNSSSNKN GHQSEEDIKD QESIESKKSV SQISVNPGKN TDKPAANLWN
SWKEYFNKPK NTANEDVTES TEDLKNRSKT SNAIQKAMMG LGLHYKPDAE TDQQSEEVGN
SEDNEDDDTD EDSEDDDDDG GDDDDSEDDD DDDDGEGDEN GDDGEGDENG DDGEGDENGD
KEDSQDFSNG FTDVTNINTD HDKENEQNFE GNAENFNEDE TVADKDIEGG PESNKNSDSK
TDIYENDRNV EDSSKTRNTV KKSNEEGGAN DAAIGNCVQD EEKL
