ID   RRAGA_DICDI             Reviewed;         301 AA.
AC   Q54IK1;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Ras-related GTP-binding protein A {ECO:0000305};
GN   Name=ragA; ORFNames=DDB_G0288701;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Guanine nucleotide-binding protein that plays a crucial role
CC       in the cellular response to amino acid availability through regulation
CC       of the TOR signaling cascade. {ECO:0000250|UniProtKB:Q7L523}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q7L523}. Nucleus
CC       {ECO:0000250|UniProtKB:Q7L523}. Lysosome
CC       {ECO:0000250|UniProtKB:Q7L523}.
CC   -!- SIMILARITY: Belongs to the GTR/RAG GTP-binding protein family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000120; EAL63082.1; -; Genomic_DNA.
DR   RefSeq; XP_636585.1; XM_631493.1.
DR   AlphaFoldDB; Q54IK1; -.
DR   SMR; Q54IK1; -.
DR   STRING; 44689.DDB0230043; -.
DR   PaxDb; Q54IK1; -.
DR   PRIDE; Q54IK1; -.
DR   EnsemblProtists; EAL63082; EAL63082; DDB_G0288701.
DR   GeneID; 8626758; -.
DR   KEGG; ddi:DDB_G0288701; -.
DR   dictyBase; DDB_G0288701; ragA.
DR   eggNOG; KOG3886; Eukaryota.
DR   HOGENOM; CLU_044099_1_0_1; -.
DR   InParanoid; Q54IK1; -.
DR   OMA; LIPNMQD; -.
DR   PhylomeDB; Q54IK1; -.
DR   Reactome; R-DDI-1632852; Macroautophagy.
DR   Reactome; R-DDI-165159; MTOR signalling.
DR   Reactome; R-DDI-166208; mTORC1-mediated signalling.
DR   Reactome; R-DDI-380972; Energy dependent regulation of mTOR by LKB1-AMPK.
DR   Reactome; R-DDI-5628897; TP53 Regulates Metabolic Genes.
DR   Reactome; R-DDI-8866654; E3 ubiquitin ligases ubiquitinate target proteins.
DR   Reactome; R-DDI-8943724; Regulation of PTEN gene transcription.
DR   Reactome; R-DDI-9639288; Amino acids regulate mTORC1.
DR   PRO; PR:Q54IK1; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:1990131; C:Gtr1-Gtr2 GTPase complex; IBA:GO_Central.
DR   GO; GO:0005764; C:lysosome; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR   GO; GO:0071230; P:cellular response to amino acid stimulus; IBA:GO_Central.
DR   GO; GO:0009267; P:cellular response to starvation; IBA:GO_Central.
DR   GO; GO:0006817; P:phosphate ion transport; ISS:dictyBase.
DR   GO; GO:0032008; P:positive regulation of TOR signaling; IBA:GO_Central.
DR   GO; GO:0010506; P:regulation of autophagy; IBA:GO_Central.
DR   CDD; cd11384; RagA_like; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR006762; Gtr1_RagA.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR039397; RagA/B.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   PANTHER; PTHR11259; PTHR11259; 1.
DR   Pfam; PF04670; Gtr1_RagA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; GTP-binding; Lysosome; Nucleotide-binding; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..301
FT                   /note="Ras-related GTP-binding protein A"
FT                   /id="PRO_0000327795"
FT   BINDING         9..16
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         57..61
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         122..125
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   301 AA;  34828 MW;  D7BD3ADF30103BBB CRC64;
     MKRKVLLMGR SESGKTSMRS IIFANYIARD TMRLGVTIDV EHSHVRFLGN LVLNLWDCGG
     QEGFLESYLT TQRDHIFRNV EVLIYVFDIE SREHQKDIKN YKSCIEAISQ NSKDAKIFCL
     IHKMDLVPED QRDTLFKNKE QEIRQASLPL KPTCFRTSIW DETLYKAWSS IVYSLIPNVK
     VLEYNLDKFC KICEADEVVL FEKATFLVIS HSARKVHKDV HRFEKISTII KQFFLSCSKS
     QANFQAMEVR NSNFAAFIDA FTSNTYIMVI MSDPTIESSA TLLNIQVAKS HFEKFINSTS
     N