RRC1_ARATH
ID RRC1_ARATH Reviewed; 946 AA.
AC Q9C5J3;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 148.
DE RecName: Full=Protein RRC1 {ECO:0000303|PubMed:22324426};
DE AltName: Full=Reduced red-light responses in cry1cry2 background 1 {ECO:0000303|PubMed:22324426};
GN Name=RRC1 {ECO:0000303|PubMed:22324426};
GN OrderedLocusNames=At5g25060 {ECO:0000312|Araport:AT5G25060};
GN ORFNames=T11H3.70 {ECO:0000312|EMBL:AC005964};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAK25922.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RX PubMed=22324426; DOI=10.1111/j.1365-313x.2012.04937.x;
RA Shikata H., Shibata M., Ushijima T., Nakashima M., Kong S.G., Matsuoka K.,
RA Lin C., Matsushita T.;
RT "The RS domain of Arabidopsis splicing factor RRC1 is required for
RT phytochrome B signal transduction.";
RL Plant J. 70:727-738(2012).
RN [5]
RP DOMAIN.
RX PubMed=22751357; DOI=10.4161/psb.20854;
RA Shikata H., Nakashima M., Matsuoka K., Matsushita T.;
RT "Deletion of the RS domain of RRC1 impairs phytochrome B signaling in
RT Arabidopsis.";
RL Plant Signal. Behav. 7:933-936(2012).
RN [6]
RP STRUCTURE BY NMR OF 789-842.
RA He F., Muto Y., Inoue M., Kigawa T., Shirouzu M., Terada T., Yokoyama S.;
RT "Solution structure of the cwf21 domain in protein AAK25922.";
RL Submitted (DEC-2006) to the PDB data bank.
CC -!- FUNCTION: SR-like splicing factor required for phytochrome B (phyB)
CC signal transduction and involved in phyB-dependent alternative
CC splicing. {ECO:0000269|PubMed:22324426}.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000269|PubMed:22324426}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, inflorescence stems, roots,
CC flower buds, open flowers and siliques. {ECO:0000269|PubMed:22324426}.
CC -!- DOMAIN: The RS domain (871-946) is required for phytochrome B signal
CC transduction through alternative splicing regulation.
CC {ECO:0000269|PubMed:22751357}.
CC -!- DISRUPTION PHENOTYPE: Dwarfism and sterile flowers when homozygous and
CC semi-sterility when heterozygous under normal growth conditions.
CC Reduced inhibition of hypocotyl elongation under continuous red light.
CC {ECO:0000269|PubMed:22324426}.
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DR EMBL; AC005964; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED93395.1; -; Genomic_DNA.
DR EMBL; AF360212; AAK25922.1; -; mRNA.
DR EMBL; AY040060; AAK64118.1; -; mRNA.
DR RefSeq; NP_568464.1; NM_122415.2.
DR PDB; 2E62; NMR; -; A=789-842.
DR PDBsum; 2E62; -.
DR AlphaFoldDB; Q9C5J3; -.
DR SMR; Q9C5J3; -.
DR IntAct; Q9C5J3; 2.
DR STRING; 3702.AT5G25060.1; -.
DR iPTMnet; Q9C5J3; -.
DR PaxDb; Q9C5J3; -.
DR PRIDE; Q9C5J3; -.
DR ProteomicsDB; 236266; -.
DR EnsemblPlants; AT5G25060.1; AT5G25060.1; AT5G25060.
DR GeneID; 832577; -.
DR Gramene; AT5G25060.1; AT5G25060.1; AT5G25060.
DR KEGG; ath:AT5G25060; -.
DR Araport; AT5G25060; -.
DR TAIR; locus:2179295; AT5G25060.
DR eggNOG; KOG0151; Eukaryota.
DR HOGENOM; CLU_010743_2_0_1; -.
DR InParanoid; Q9C5J3; -.
DR OMA; VTTNLYI; -.
DR OrthoDB; 523911at2759; -.
DR PhylomeDB; Q9C5J3; -.
DR EvolutionaryTrace; Q9C5J3; -.
DR PRO; PR:Q9C5J3; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9C5J3; baseline and differential.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR CDD; cd12223; RRM_SR140; 1.
DR Gene3D; 1.10.10.790; -; 1.
DR Gene3D; 1.25.40.90; -; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR006569; CID_dom.
DR InterPro; IPR008942; ENTH_VHS.
DR InterPro; IPR013170; mRNA_splic_Cwf21_dom.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR003034; SAP_dom.
DR InterPro; IPR035009; SR140_RRM.
DR InterPro; IPR000061; Surp.
DR InterPro; IPR035967; SWAP/Surp_sf.
DR Pfam; PF04818; CID; 1.
DR Pfam; PF08312; cwf21; 1.
DR Pfam; PF00076; RRM_1; 1.
DR Pfam; PF01805; Surp; 1.
DR SMART; SM01115; cwf21; 1.
DR SMART; SM00582; RPR; 1.
DR SMART; SM00360; RRM; 1.
DR SMART; SM00648; SWAP; 1.
DR SUPFAM; SSF109905; SSF109905; 1.
DR SUPFAM; SSF48464; SSF48464; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS51391; CID; 1.
DR PROSITE; PS50102; RRM; 1.
DR PROSITE; PS50800; SAP; 1.
DR PROSITE; PS50128; SURP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW RNA-binding.
FT CHAIN 1..946
FT /note="Protein RRC1"
FT /id="PRO_0000436038"
FT DOMAIN 179..260
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REPEAT 329..372
FT /note="SURP motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00263"
FT DOMAIN 437..582
FT /note="CID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00724"
FT DOMAIN 631..665
FT /note="SAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00186"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 41..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 740..797
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 836..946
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..29
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 41..55
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 64..79
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 98..168
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 740..758
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 774..797
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 851..946
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 792..816
FT /evidence="ECO:0007829|PDB:2E62"
FT HELIX 821..838
FT /evidence="ECO:0007829|PDB:2E62"
SQ SEQUENCE 946 AA; 108286 MW; B531054388ABEB8B CRC64;
MSSFSITRKK TPFQKHREEE EARKKKAEDE TARLYQEFVE SFQGDNATTK TFVRGGTINP
GDKPKVDSEG EKSKDGGSVS KKGSRYVPSF LPPPLASKGK EPEKKREEER PREREKGKTR
NIDNFMEELK REQEMRERRN QDRDRQGDSS PSSRFDELPD DFDPSGRPGS FDDGDPQTTN
LYVGNLSPKV DENFLLRTFG RFGPIASVKI MWPRTDEEKR RQRNCGFVSF MNRADGQAAK
DEMQGIIVYE YELKIGWGKA VSLPSQALPA PPPGHMAIRS KEGCNLVFSG QTGPPIITSV
PNQNSELVLT PNVPDITVVT PEDEHLRHVI DTLALYVLDG ECAFEQAIME RGRGNPLFKF
MFELGSKEHT YYVWRLYSFA QGDTLQRWRT EPYIMITGSG RWIPPPLPVT RTQEHEKESA
STYAAGRTRR AEVERTLTDP QRDEFEDMLR ALTLERSQIK EAMGFALDNA DAAGEVVEVL
TESLTLKETS IPTKVARLML VSDILHNSSA RVKNASAYRT KFEATLPDIM ESFNDLYRSI
TGRITAEALK ERVLKVLQVW ADWFLFSDAY IYGLRSTFLR SGVSGVTSFH SICGDAPEIE
NKSYADNMSD IGKINPDAAL AIGKGAARQE LMNLPIAELE RRCRHNGLSL VGGRVMMVTR
LLSLEDTEKQ RGYEAVDEIP KHPQNHSTWE EVKSEREHIK NSYAEVEMKE PVNLPTTIPI
PQPELKAFVG KEKNELILPA SKWARDDDEA DDEQKRSSSS GSDNTGGITF KADGEDLKGN
DCVRAQPDNG MDEEQRQKRR RIEVALIEYR ETLEEQGMKN PEEIERKVEI NRKRLEVDYG
LSGPNEGNRN QKSIIERKEK REDSQESSKK RHRGENKSQS PPRKSSTRER DHDLGRDRDR
ERHRDRDRQH DLNRDRDRRE KSSSHDRDDN DRSKERDRDW RRRGTR
