RRE1_SYNY3
ID RRE1_SYNY3 Reviewed; 231 AA.
AC P72781;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 02-JUN-2021, sequence version 2.
DT 25-MAY-2022, entry version 149.
DE RecName: Full=Response regulator Rre1 {ECO:0000303|PubMed:15471853};
GN Name=rre1 {ECO:0000303|PubMed:15471853}; Synonyms=ycf29;
GN OrderedLocusNames=slr1783 {ECO:0000312|EMBL:BAA16796.1};
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
RN [2]
RP FUNCTION IN HYPEROSMOTIC STRESS RESPONSE, REGULON, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=15471853; DOI=10.1074/jbc.m410162200;
RA Paithoonrangsarid K., Shoumskaya M.A., Kanesaki Y., Satoh S., Tabata S.,
RA Los D.A., Zinchenko V.V., Hayashi H., Tanticharoen M., Suzuki I.,
RA Murata N.;
RT "Five histidine kinases perceive osmotic stress and regulate distinct sets
RT of genes in Synechocystis.";
RL J. Biol. Chem. 279:53078-53086(2004).
RN [3]
RP FUNCTION IN SALT STRESS RESPONSE, REGULON, AND DISRUPTION PHENOTYPE.
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=15805106; DOI=10.1074/jbc.m412174200;
RA Shoumskaya M.A., Paithoonrangsarid K., Kanesaki Y., Los D.A.,
RA Zinchenko V.V., Tanticharoen M., Suzuki I., Murata N.;
RT "Identical Hik-Rre systems are involved in perception and transduction of
RT salt signals and hyperosmotic signals but regulate the expression of
RT individual genes to different extents in synechocystis.";
RL J. Biol. Chem. 280:21531-21538(2005).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND DNA-BINDING.
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=19411329; DOI=10.1128/jb.00183-09;
RA Vidal R., Lopez-Maury L., Guerrero M.G., Florencio F.J.;
RT "Characterization of an alcohol dehydrogenase from the Cyanobacterium
RT Synechocystis sp. strain PCC 6803 that responds to environmental stress
RT conditions via the Hik34-Rre1 two-component system.";
RL J. Bacteriol. 191:4383-4391(2009).
RN [5]
RP SEQUENCE REVISION TO N-TERMINUS, PHOSPHORYLATED BY HIK2, AND DNA-BINDING.
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=25714549; DOI=10.1093/femsle/fnv030;
RA Vidal R.;
RT "Identification of the correct form of the mis-annotated response regulator
RT Rre1 from the cyanobacterium Synechocystis sp. PCC 6803.";
RL FEMS Microbiol. Lett. 362:0-0(2015).
RN [6]
RP INTERACTION WITH HIK2.
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=26904089; DOI=10.3389/fpls.2016.00137;
RA Ibrahim I.M., Puthiyaveetil S., Allen J.F.;
RT "A Two-Component Regulatory System in Transcriptional Control of
RT Photosystem Stoichiometry: Redox-Dependent and Sodium Ion-Dependent
RT Phosphoryl Transfer from Cyanobacterial Histidine Kinase Hik2 to Response
RT Regulators Rre1 and RppA.";
RL Front. Plant Sci. 7:137-137(2016).
CC -!- FUNCTION: Member of at least 2 two-component regulatory systems
CC Hik2/Rre1 and Hik34/Rre1. Responds to hyperosmotic stress, regulates
CC expression of at least 24 genes including dnaK2 and hspA with Hik34 and
CC sigB (sll0306), sll0528, slr1119, slr0852 and ssr3188 with Hik2
CC (Probable). Responds to salt stress, regulates expression of at least
CC 24 genes including adhA, dnaK2 and hspA with Hik34 (PubMed:15805106,
CC PubMed:19411329). Binds the adhA promoter (PubMed:19411329).
CC Phosphorylated by Hik2 in vitro (PubMed:25714549, PubMed:26904089).
CC Phosphorylated protein has 10-fold higher affinity for DNA than
CC unphosphorylated protein (PubMed:25714549).
CC {ECO:0000269|PubMed:15805106, ECO:0000269|PubMed:19411329,
CC ECO:0000269|PubMed:25714549, ECO:0000269|PubMed:26904089,
CC ECO:0000305|PubMed:15471853}.
CC -!- SUBUNIT: Interacts with histidine kinase Hik2; may accept phosphate
CC from Hik2. {ECO:0000269|PubMed:26904089}.
CC -!- DISRUPTION PHENOTYPE: Loss or reduction of expression of about 30 genes
CC in response to hyperosmotic (0.5 M sorbitol) or salt (0.5 M NaCl)
CC stress. Most of these genes encode known heat-shock proteins.
CC {ECO:0000269|PubMed:15471853, ECO:0000269|PubMed:15805106,
CC ECO:0000269|PubMed:19411329}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA16796.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305|PubMed:25714549};
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DR EMBL; BA000022; BAA16796.1; ALT_INIT; Genomic_DNA.
DR PIR; S74644; S74644.
DR AlphaFoldDB; P72781; -.
DR SMR; P72781; -.
DR IntAct; P72781; 4.
DR STRING; 1148.1651869; -.
DR PaxDb; P72781; -.
DR EnsemblBacteria; BAA16796; BAA16796; BAA16796.
DR KEGG; syn:slr1783; -.
DR eggNOG; COG2197; Bacteria.
DR InParanoid; P72781; -.
DR PhylomeDB; P72781; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd06170; LuxR_C_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF00196; GerE; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..231
FT /note="Response regulator Rre1"
FT /id="PRO_0000453143"
FT DOMAIN 6..123
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 163..228
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 187..206
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT MOD_RES 56
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 231 AA; 25767 MW; 2F62900E1EDBEEFC CRC64;
MAEPISLLLV DDEPGVRESV QAFLEDSGDF KVDLAANATE AWDYLQHHLP ALVISDIMMP
QVDGYQFLQK LREDARFQSL PVVFLTARGM TGDRIQGYQT GCDAFLSKPF DPDELEAIVR
NLLARQQASS DAGSESAKLQ EIYQEIRALK EQIGQPSGIH TTPSPIKLDF TPREQSVLDL
VSQGLMNKEI AAQLKTSVRN VEKYVSRLFT KTGTNSRTEL VRFALQHGLT E
