RRF1_YEAST
ID RRF1_YEAST Reviewed; 230 AA.
AC P38771; D3DKY5;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Ribosome-recycling factor, mitochondrial;
DE Short=RRF;
DE AltName: Full=Ribosome-releasing factor, mitochondrial;
DE Flags: Precursor;
GN Name=RRF1; Synonyms=FIL1, KIM4; OrderedLocusNames=YHR038W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=MT8-1;
RX PubMed=9746366; DOI=10.1046/j.1432-1327.1998.2560212.x;
RA Kanai T., Takeshita S., Atomi H., Umemura K., Ueda M., Tanaka A.;
RT "A regulatory factor, Fil1p, involved in derepression of the isocitrate
RT lyase gene in Saccharomyces cerevisiae -- a possible mitochondrial protein
RT necessary for protein synthesis in mitochondria.";
RL Eur. J. Biochem. 256:212-220(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SIMILARITY TO RRF.
RX PubMed=8563640; DOI=10.1002/pro.5560041121;
RA Ouzounis C., Bork P., Casari G., Sander C.;
RT "New protein functions in yeast chromosome VIII.";
RL Protein Sci. 4:2424-2428(1995).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=12853640; DOI=10.1093/nar/gkg449;
RA Teyssier E., Hirokawa G., Tretiakova A., Jameson B., Kaji A., Kaji H.;
RT "Temperature-sensitive mutation in yeast mitochondrial ribosome recycling
RT factor (RRF).";
RL Nucleic Acids Res. 31:4218-4226(2003).
CC -!- FUNCTION: Necessary for protein synthesis in mitochondria. Functions as
CC a ribosome recycling factor in mitochondria.
CC {ECO:0000269|PubMed:12853640}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:12853640}.
CC -!- MISCELLANEOUS: Present with 1440 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the RRF family. {ECO:0000305}.
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DR EMBL; AB016033; BAA31687.1; -; Genomic_DNA.
DR EMBL; U00062; AAB68906.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06729.1; -; Genomic_DNA.
DR PIR; S46737; S46737.
DR RefSeq; NP_011903.1; NM_001179168.1.
DR AlphaFoldDB; P38771; -.
DR SMR; P38771; -.
DR BioGRID; 36469; 59.
DR DIP; DIP-1693N; -.
DR IntAct; P38771; 1.
DR MINT; P38771; -.
DR STRING; 4932.YHR038W; -.
DR MaxQB; P38771; -.
DR PaxDb; P38771; -.
DR PRIDE; P38771; -.
DR EnsemblFungi; YHR038W_mRNA; YHR038W; YHR038W.
DR GeneID; 856433; -.
DR KEGG; sce:YHR038W; -.
DR SGD; S000001080; RRF1.
DR VEuPathDB; FungiDB:YHR038W; -.
DR eggNOG; KOG4759; Eukaryota.
DR GeneTree; ENSGT00390000005084; -.
DR HOGENOM; CLU_085410_0_0_1; -.
DR InParanoid; P38771; -.
DR OMA; VFDPKDT; -.
DR BioCyc; YEAST:G3O-31097-MON; -.
DR PRO; PR:P38771; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38771; protein.
DR GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR GO; GO:0043023; F:ribosomal large subunit binding; IBA:GO_Central.
DR GO; GO:0003747; F:translation release factor activity; ISO:SGD.
DR GO; GO:0032543; P:mitochondrial translation; IMP:SGD.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR CDD; cd00520; RRF; 1.
DR Gene3D; 1.10.132.20; -; 1.
DR InterPro; IPR002661; Ribosome_recyc_fac.
DR InterPro; IPR023584; Ribosome_recyc_fac_dom.
DR InterPro; IPR036191; RRF_sf.
DR PANTHER; PTHR20982; PTHR20982; 1.
DR Pfam; PF01765; RRF; 1.
DR SUPFAM; SSF55194; SSF55194; 1.
PE 1: Evidence at protein level;
KW Mitochondrion; Protein biosynthesis; Reference proteome; Transit peptide.
FT TRANSIT 1..24
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 25..230
FT /note="Ribosome-recycling factor, mitochondrial"
FT /id="PRO_0000031091"
SQ SEQUENCE 230 AA; 26407 MW; 9CBCD8C5F86F3008 CRC64;
MILTTARLNC RPVTVPRLFN RSFSQSFIIL KKKSSTPTEK VEEDEIDVNE LLKKAETQFK
KTLEIQKQKM NEIKQGNFNP KVFNSLVFKN NRKFTDIATT SLKGKNALLI TVFDPKDVKT
VISGVLAANL NLTPERVPNN DLQLKVSLPP PTTESRLKVA KDLKRVFEEY KQSSLKDSLG
TIRGSILKEF KSFKKDDAVR KAERDLEKLH KDYVNKLHDQ FQKVEKSIVK
