RRF2M_CANGA
ID RRF2M_CANGA Reviewed; 807 AA.
AC Q6FLG2;
DT 13-OCT-2009, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Ribosome-releasing factor 2, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03059};
DE Short=RRF2mt {ECO:0000255|HAMAP-Rule:MF_03059};
DE AltName: Full=Elongation factor G 2, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03059};
DE Short=EF-G2mt {ECO:0000255|HAMAP-Rule:MF_03059};
DE Short=mEF-G 2 {ECO:0000255|HAMAP-Rule:MF_03059};
GN Name=MEF2 {ECO:0000255|HAMAP-Rule:MF_03059};
GN OrderedLocusNames=CAGL0L03652g;
OS Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS Y-65) (Yeast) (Torulopsis glabrata).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC Nakaseomyces/Candida clade.
OX NCBI_TaxID=284593;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
CC -!- FUNCTION: Mitochondrial GTPase that mediates the disassembly of
CC ribosomes from messenger RNA at the termination of mitochondrial
CC protein biosynthesis. Not involved in the GTP-dependent ribosomal
CC translocation step during translation elongation. {ECO:0000255|HAMAP-
CC Rule:MF_03059}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000255|HAMAP-Rule:MF_03059}.
CC -!- MISCELLANEOUS: This protein may be expected to contain an N-terminal
CC transit peptide but none has been predicted. {ECO:0000255|HAMAP-
CC Rule:MF_03059}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-G/EF-2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_03059}.
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DR EMBL; CR380958; CAG61902.1; -; Genomic_DNA.
DR RefSeq; XP_448932.1; XM_448932.1.
DR AlphaFoldDB; Q6FLG2; -.
DR SMR; Q6FLG2; -.
DR STRING; 5478.XP_448932.1; -.
DR EnsemblFungi; CAG61902; CAG61902; CAGL0L03652g.
DR GeneID; 2890538; -.
DR KEGG; cgr:CAGL0L03652g; -.
DR CGD; CAL0135510; CAGL0L03652g.
DR VEuPathDB; FungiDB:CAGL0L03652g; -.
DR eggNOG; KOG0465; Eukaryota.
DR HOGENOM; CLU_002794_4_1_1; -.
DR InParanoid; Q6FLG2; -.
DR OMA; AVCQIPW; -.
DR Proteomes; UP000002428; Chromosome L.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IEA:EnsemblFungi.
DR GO; GO:0032543; P:mitochondrial translation; IEA:UniProtKB-UniRule.
DR GO; GO:0051881; P:regulation of mitochondrial membrane potential; IEA:EnsemblFungi.
DR GO; GO:0032790; P:ribosome disassembly; IEA:UniProtKB-UniRule.
DR CDD; cd16262; EFG_III; 1.
DR CDD; cd03713; EFG_mtEFG_C; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_03059; mEF_G_2; 1.
DR InterPro; IPR030851; EFG2.
DR InterPro; IPR041095; EFG_II.
DR InterPro; IPR009022; EFG_III.
DR InterPro; IPR035647; EFG_III/V.
DR InterPro; IPR035649; EFG_V.
DR InterPro; IPR000640; EFG_V-like.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR Pfam; PF00679; EFG_C; 1.
DR Pfam; PF14492; EFG_III; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SMART; SM00838; EFG_C; 1.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54980; SSF54980; 2.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 3: Inferred from homology;
KW GTP-binding; Mitochondrion; Nucleotide-binding; Protein biosynthesis;
KW Reference proteome.
FT CHAIN 1..807
FT /note="Ribosome-releasing factor 2, mitochondrial"
FT /id="PRO_0000385613"
FT DOMAIN 21..319
FT /note="tr-type G"
FT BINDING 30..37
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03059"
FT BINDING 101..105
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03059"
FT BINDING 153..156
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03059"
SQ SEQUENCE 807 AA; 89628 MW; D18376630441EA0A CRC64;
MLRFVCGRFQ SGIRRYATTI DRFRNIGIIA HIDAGKTTTT ERMLYCAGKI KRIGDVDSGD
TVTDFLPQER DRGITIQSAA ISFQWHDSSR DNSPRVINLI DTPGHADFIF EVIRSLKVLD
GAVLILDGVA GVEAQTEKLW RYSRNIPKIC FVNKMDRVGA GFSKTLKELT YKTDRRCVVV
NFPYFEQTNQ SEDPVFCGVI DVINMKLLKW DTKANSPDAV TVVDLLELND SDNAKVEAIK
SRESMVEILG ELDDVFVQEF FDGPSEGNSL KVSAQVLNKS IRKLTIANEL TPVLAGASFK
NVGIQPLLDG IIQYLPSPLE ARLPEVNPKD IPVVHDPKVG ALINKQNNLC VALAFKVTHD
QIRGLMVFVR IYSGVLNSGN TVMNTTTGEK FRIGKLVIMQ ADQAQEVKSL SPGQIGVLTG
ATITNKVATG DTIISHSIKK DGIRSFGEKE SQLKVNAIQI PPPVFSVIVE PKSLGNKKLI
EDALKEITME DPSLHVIIDE ETGQTVLNGM GQLHLEIAKF RLVNEMKVPV DFGKIAVSYK
ETLELPTEVV TFKDERGFHF SLSVESFEKD TINIEERSGA SCFSVDNDEN YLIIEGFQDR
LQKSNWAYQI PIRNFVTTIL SSAMASLQSA GKVGHLPLYS CAVRLKKDWD FPLDLQSPAE
LLSITRSLIT EALQTVDVSN YTLLEPVMDA HISVPQNDIG TVMQDLCSAR TATISSLDDE
NVSSSDKLME LESQSKHLYY PTDSTLGTAK IQDDKLKTIH AICPLKEMVT YSSKLRSLTK
GRGELHMDYK GMSKVSQERI PDILQEL
