RRF2M_YEAST
ID RRF2M_YEAST Reviewed; 819 AA.
AC P39677; D6VW82;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Ribosome-releasing factor 2, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03059};
DE Short=RRF2mt {ECO:0000255|HAMAP-Rule:MF_03059};
DE AltName: Full=Elongation factor G 2, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03059};
DE Short=EF-G2mt {ECO:0000255|HAMAP-Rule:MF_03059};
DE Short=mEF-G 2 {ECO:0000255|HAMAP-Rule:MF_03059};
DE Flags: Precursor;
GN Name=MEF2 {ECO:0000255|HAMAP-Rule:MF_03059}; OrderedLocusNames=YJL102W;
GN ORFNames=D819, J0826;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Welcsh P.L., Johnson D.R., Breitenberger C.A.;
RT "The yeast nuclear Mef2 gene encodes a second mitochondrial EF-G-like
RT protein.";
RL Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=7483851; DOI=10.1002/yea.320110909;
RA Rasmussen S.W.;
RT "A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and
RT CSD3 genes, a TCP-1-related gene, an open reading frame similar to the
RT DAL80 gene, and a tRNA(Arg).";
RL Yeast 11:873-883(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 76625 / YPH499;
RX PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E.,
RA Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P.,
RA Pfanner N., Meisinger C.;
RT "The proteome of Saccharomyces cerevisiae mitochondria.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=21414316; DOI=10.1016/j.febslet.2011.03.022;
RA Callegari S., McKinnon R.A., Andrews S., de Barros Lopes M.A.;
RT "The MEF2 gene is essential for yeast longevity, with a dual role in cell
RT respiration and maintenance of mitochondrial membrane potential.";
RL FEBS Lett. 585:1140-1146(2011).
CC -!- FUNCTION: Mitochondrial GTPase that mediates the disassembly of
CC ribosomes from messenger RNA at the termination of mitochondrial
CC protein biosynthesis. Not involved in the GTP-dependent ribosomal
CC translocation step during translation elongation (By similarity).
CC Required for respiratory growth and essential for mitochondrial genome
CC maintenance. {ECO:0000255|HAMAP-Rule:MF_03059,
CC ECO:0000269|PubMed:21414316}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000255|HAMAP-Rule:MF_03059,
CC ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:14576278}.
CC -!- DISRUPTION PHENOTYPE: Results in loss of mitochondrial DNA. Causes a
CC significant slow growth phenotype under normal growth conditions
CC (glucose) and a reduced chronological life-span (CLS). Growth on a non-
CC fermentable carbon source (gylcerol) is absent.
CC {ECO:0000269|PubMed:21414316}.
CC -!- MISCELLANEOUS: Present with 1733 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-G/EF-2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_03059}.
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DR EMBL; L25088; AAB59315.1; -; Genomic_DNA.
DR EMBL; X85021; CAA59392.1; -; Genomic_DNA.
DR EMBL; Z49377; CAA89397.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08698.1; -; Genomic_DNA.
DR PIR; S43748; S43748.
DR RefSeq; NP_012433.1; NM_001181535.1.
DR AlphaFoldDB; P39677; -.
DR SMR; P39677; -.
DR BioGRID; 33654; 300.
DR DIP; DIP-2761N; -.
DR IntAct; P39677; 2.
DR MINT; P39677; -.
DR STRING; 4932.YJL102W; -.
DR MaxQB; P39677; -.
DR PaxDb; P39677; -.
DR PRIDE; P39677; -.
DR EnsemblFungi; YJL102W_mRNA; YJL102W; YJL102W.
DR GeneID; 853342; -.
DR KEGG; sce:YJL102W; -.
DR SGD; S000003638; MEF2.
DR VEuPathDB; FungiDB:YJL102W; -.
DR eggNOG; KOG0465; Eukaryota.
DR GeneTree; ENSGT00550000074890; -.
DR HOGENOM; CLU_002794_4_1_1; -.
DR InParanoid; P39677; -.
DR OMA; AVCQIPW; -.
DR BioCyc; YEAST:G3O-31556-MON; -.
DR PRO; PR:P39677; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P39677; protein.
DR GO; GO:0005829; C:cytosol; HDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; ISS:SGD.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IMP:SGD.
DR GO; GO:0032543; P:mitochondrial translation; ISS:SGD.
DR GO; GO:0051881; P:regulation of mitochondrial membrane potential; IMP:SGD.
DR GO; GO:0032790; P:ribosome disassembly; ISS:SGD.
DR CDD; cd16262; EFG_III; 1.
DR CDD; cd03713; EFG_mtEFG_C; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_03059; mEF_G_2; 1.
DR InterPro; IPR030851; EFG2.
DR InterPro; IPR041095; EFG_II.
DR InterPro; IPR009022; EFG_III.
DR InterPro; IPR035647; EFG_III/V.
DR InterPro; IPR035649; EFG_V.
DR InterPro; IPR000640; EFG_V-like.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR Pfam; PF00679; EFG_C; 1.
DR Pfam; PF14492; EFG_III; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SMART; SM00838; EFG_C; 1.
DR SUPFAM; SSF50447; SSF50447; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF54980; SSF54980; 2.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 1: Evidence at protein level;
KW GTP-binding; Mitochondrion; Nucleotide-binding; Protein biosynthesis;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..30
FT /note="Mitochondrion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03059"
FT CHAIN 31..819
FT /note="Ribosome-releasing factor 2, mitochondrial"
FT /id="PRO_0000007453"
FT DOMAIN 39..327
FT /note="tr-type G"
FT BINDING 48..55
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03059"
FT BINDING 113..117
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03059"
FT BINDING 165..168
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03059"
SQ SEQUENCE 819 AA; 91283 MW; DDACCD85FC6F6798 CRC64;
MWKWNVRRWA GARVNISKNR LSVINVGSRY LSTARSPLSK VRNIGIIAHI DAGKTTTTER
MLYYAGISKH IGDVDTGDTI TDFLEQERSR GITIQSAAIS FPWRNTFAIN LIDTPGHIDF
TFEVIRALKV IDSCVVILDA VAGVEAQTEK VWKQSKSKPK ICFINKMDRM GASFNHTVND
LINKFMRGTT TKPVLVNIPY YRKQPTSNDY VFQGVIDVVN GKRLTWNPEN PDEIIVDELD
GTSLEQCNRC RESMIETLTE YDEDLVQHFL EEAEGDYSKV SAQFLNASIR KLTMKNMIVP
VLCGASFKNI GVQPLLDAIV NYLPSPIEAE LPELNDKTVP MKYDPKVGCL VNNNKNLCIA
LAFKVITDPI RGKQIFIRIY SGTLNSGNTV YNSTTGEKFK LGKLLIPHAG TSQPVNILTA
GQIGLLTGST VENNISTGDT LITHSSKKDG LKSLDKKKEL TLKINSIFIP PPVFGVSIEP
RTLSNKKSME EALNTLITED PSLSISQNDE TGQTVLNGMG ELHLEIAKDR LVNDLKADVE
FGQLMVSYKE TINSETNIET YESDDGYRFS LSLLPNSDAL PNCLAYPLGV NENFLIMEKN
GNWDKEWKYQ VSFESILNSI IASCIVGLQR GGKIANFPLY ACSIKINSDW SVPPDIETPQ
EILKITRNLI FKALNDLKPE KYNLLEPIMN LDLTIPQSDV GTVLQDLTGA RKAQILSIED
ESSVSNSGAS TCNSPENSNR IYIPSDAVTT LHATKDKKNT QETSSNVKKI IKAKVPLREI
TTYTNKLRSL SQGRGEFNIE YSDMEKVTND RLQSILHDL
