ID   RRFC_DAUCA              Reviewed;         227 AA.
AC   P37706;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   29-SEP-2021, entry version 82.
DE   RecName: Full=Ribosome-recycling factor, chloroplastic;
DE            Short=RRF;
DE   AltName: Full=Nuclear located protein D2;
DE   AltName: Full=Ribosome-releasing factor, chloroplastic;
DE   Flags: Precursor; Fragment;
GN   Name=RRF;
OS   Daucus carota (Wild carrot).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC   Daucus; Daucus sect. Daucus.
OX   NCBI_TaxID=4039;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Schrader S., Kaldenhoff R., Richter G.;
RL   Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   IDENTIFICATION OF PROBABLE FRAMESHIFT.
RX   PubMed=10318906; DOI=10.1073/pnas.96.10.5464;
RA   Rolland N., Janosi L., Block M.A., Shuda M., Teyssier E., Miege C.,
RA   Cheniclet C., Carde J.-P., Kaji A., Joyard J.;
RT   "Plant ribosome recycling factor homologue is a chloroplastic protein and
RT   is bactericidal in Escherichia coli carrying temperature-sensitive
RT   Ribosome-recycling factor.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:5464-5469(1999).
CC   -!- FUNCTION: Responsible for the release of ribosomes from messenger RNA
CC       at the termination of chloroplastic protein biosynthesis.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RRF family. {ECO:0000305}.
CC   -!- CAUTION: Was originally (Ref.1) submitted as a nuclear protein when it
CC       seems to be chloroplastic (By similarity with the spinach sequence).
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA51077.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; X72384; CAA51077.1; ALT_FRAME; mRNA.
DR   PIR; S32716; S32716.
DR   PRIDE; P37706; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
DR   CDD; cd00520; RRF; 1.
DR   Gene3D; 1.10.132.20; -; 1.
DR   HAMAP; MF_00040; RRF; 1.
DR   InterPro; IPR002661; Ribosome_recyc_fac.
DR   InterPro; IPR023584; Ribosome_recyc_fac_dom.
DR   InterPro; IPR036191; RRF_sf.
DR   PANTHER; PTHR20982; PTHR20982; 1.
DR   Pfam; PF01765; RRF; 1.
DR   SUPFAM; SSF55194; SSF55194; 1.
DR   TIGRFAMs; TIGR00496; frr; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Coiled coil; Plastid; Protein biosynthesis; Transit peptide.
FT   TRANSIT         <1..34
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000250"
FT   CHAIN           35..227
FT                   /note="Ribosome-recycling factor, chloroplastic"
FT                   /id="PRO_0000031084"
FT   COILED          167..212
FT                   /evidence="ECO:0000255"
FT   NON_TER         1
SQ   SEQUENCE   227 AA;  25598 MW;  6375F39F387276E4 CRC64;
     WTAAANYVKI KVGTGKFARK TVVLSQKRTG TLKCATMEEI EAEKSLIEKS VKERMEKTIE
     NVKASFNSIR TXRSNPDMLD KIKVEYYGTP TSLKSIAQIS TPDSSSLLVN PYDKSSLKDI
     EKAIVNSDLG ITPNNDGDVI RLSIPQLTAD RRKELSKIVA KQAEEGKVAL RNIRRDAIKS
     YDKLEKEKKL SEDNVKDLSS DLQKVIDEYI KKVDSIFKQK EKELMTV