ID   RRFC_SPIOL              Reviewed;         271 AA.
AC   P82231; Q9XG97;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Ribosome-recycling factor, chloroplastic;
DE            Short=RRF;
DE   AltName: Full=CpFrr;
DE   AltName: Full=RRFHCP;
DE   AltName: Full=Ribosome-releasing factor, chloroplastic;
DE   Flags: Precursor;
GN   Name=RRF; Synonyms=FRRHCP;
OS   Spinacia oleracea (Spinach).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX   NCBI_TaxID=3562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 79-88.
RC   TISSUE=Leaf;
RX   PubMed=10318906; DOI=10.1073/pnas.96.10.5464;
RA   Rolland N., Janosi L., Block M.A., Shuda M., Teyssier E., Miege C.,
RA   Cheniclet C., Carde J.-P., Kaji A., Joyard J.;
RT   "Plant ribosome recycling factor homologue is a chloroplastic protein and
RT   is bactericidal in Escherichia coli carrying temperature-sensitive
RT   Ribosome-recycling factor.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:5464-5469(1999).
RN   [2]
RP   PROTEIN SEQUENCE OF 79-99 AND 194-217.
RC   STRAIN=cv. Alwaro; TISSUE=Leaf;
RX   PubMed=10874046; DOI=10.1074/jbc.m005012200;
RA   Yamaguchi K., Subramanian A.R.;
RT   "The plastid ribosomal proteins. Identification of all the proteins in the
RT   50S subunit of an organelle ribosome (chloroplast).";
RL   J. Biol. Chem. 275:28466-28482(2000).
RN   [3]
RP   MODELING ON THE 70S RIBOSOME, AND INTERACTION WITH PSRP1.
RX   PubMed=18042701; DOI=10.1073/pnas.0709856104;
RA   Sharma M.R., Wilson D.N., Datta P.P., Barat C., Schluenzen F., Fucini P.,
RA   Agrawal R.K.;
RT   "Cryo-EM study of the spinach chloroplast ribosome reveals the structural
RT   and functional roles of plastid-specific ribosomal proteins.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:19315-19320(2007).
CC   -!- FUNCTION: Responsible for the release of ribosomes from messenger RNA
CC       at the termination of chloroplastic protein biosynthesis.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Modeling onto the 70S ribosome suggests it binds to PSRP1.
CC       {ECO:0000269|PubMed:18042701}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma. Note=The major part
CC       was found in the stroma and some protein was in the envelope
CC       subfraction, but no protein was detected in the thylakoids.
CC   -!- TISSUE SPECIFICITY: Restricted to photosynthetic tissues.
CC   -!- MISCELLANEOUS: Expression seems to be light-independent.
CC   -!- SIMILARITY: Belongs to the RRF family. {ECO:0000305}.
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DR   EMBL; AJ133751; CAB41419.1; -; mRNA.
DR   PDB; 5MLC; EM; 3.90 A; 9=1-271.
DR   PDB; 6ERI; EM; 3.00 A; Az=79-269.
DR   PDBsum; 5MLC; -.
DR   PDBsum; 6ERI; -.
DR   AlphaFoldDB; P82231; -.
DR   SMR; P82231; -.
DR   IntAct; P82231; 1.
DR   PRIDE; P82231; -.
DR   OrthoDB; 1472838at2759; -.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
DR   CDD; cd00520; RRF; 1.
DR   Gene3D; 1.10.132.20; -; 1.
DR   HAMAP; MF_00040; RRF; 1.
DR   InterPro; IPR002661; Ribosome_recyc_fac.
DR   InterPro; IPR023584; Ribosome_recyc_fac_dom.
DR   InterPro; IPR036191; RRF_sf.
DR   PANTHER; PTHR20982; PTHR20982; 1.
DR   Pfam; PF01765; RRF; 1.
DR   SUPFAM; SSF55194; SSF55194; 1.
DR   TIGRFAMs; TIGR00496; frr; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chloroplast; Coiled coil; Direct protein sequencing; Plastid;
KW   Protein biosynthesis; Transit peptide.
FT   TRANSIT         1..78
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000269|PubMed:10318906,
FT                   ECO:0000269|PubMed:10874046"
FT   CHAIN           79..271
FT                   /note="Ribosome-recycling factor, chloroplastic"
FT                   /id="PRO_0000031085"
FT   COILED          213..260
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   271 AA;  30431 MW;  F7D3012BB90AD175 CRC64;
     MAASSLSSAT SYLHSFRRRN SCVSLQGVSD MECNIARTNV SVWRSSANYV RMDCGVKKFS
     GKAVVVKQLQ NRAGTFRCAT MEEVEAEKSL IETNTKQRME KTIETIRSNF NSVRTNRASP
     TMLDRIEVEY YGTPVSLKSI AQISTPDASS LLISPYDKSS LKAIEKAIVT SQLGVSPNND
     GEVIRLSLPP LTSDRRKELA KVVSKLAEEG KVAVRNIRRD ALKSYEKLEK EKKLSEDNVK
     DLSADLQKLT DEYMKKVESI YKQKEQELMK V