RRFM_HUMAN
ID RRFM_HUMAN Reviewed; 262 AA.
AC Q96E11; A8K6D8; A8K6Z4; B7Z4X5; B7Z6P7; Q5RKT1; Q5T7T0; Q5T7T1; Q5T7T2;
AC Q5T7T3; Q5T7T4; Q5T7T5;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Ribosome-recycling factor, mitochondrial;
DE Short=RRF;
DE AltName: Full=Ribosome-releasing factor, mitochondrial;
DE Flags: Precursor;
GN Name=MRRF;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 5; 6 AND 8).
RC TISSUE=Brain, Lung, Placenta, Prostate, Skeletal muscle, and Trachea;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1; 3; 4; 5; 6 AND
RP 7).
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION.
RX PubMed=9838146; DOI=10.1016/s0167-4781(98)00223-1;
RA Zhang Y., Spremulli L.L.;
RT "Identification and cloning of human mitochondrial translational release
RT factor 1 and the ribosome recycling factor.";
RL Biochim. Biophys. Acta 1443:245-250(1998).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- FUNCTION: Responsible for the release of ribosomes from messenger RNA
CC at the termination of protein biosynthesis. May increase the efficiency
CC of translation by recycling ribosomes from one round of translation to
CC another (By similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q96E11; Q6RW13-2: AGTRAP; NbExp=3; IntAct=EBI-2855755, EBI-11522760;
CC Q96E11; Q8N9N5-2: BANP; NbExp=3; IntAct=EBI-2855755, EBI-11524452;
CC Q96E11; Q96DZ9-2: CMTM5; NbExp=3; IntAct=EBI-2855755, EBI-11522780;
CC Q96E11; Q8IZU0: FAM9B; NbExp=3; IntAct=EBI-2855755, EBI-10175124;
CC Q96E11; Q13643: FHL3; NbExp=3; IntAct=EBI-2855755, EBI-741101;
CC Q96E11; Q6A162: KRT40; NbExp=3; IntAct=EBI-2855755, EBI-10171697;
CC Q96E11; P08247: SYP; NbExp=3; IntAct=EBI-2855755, EBI-9071725;
CC Q96E11; Q9Y4P8-4: WIPI2; NbExp=3; IntAct=EBI-2855755, EBI-12205107;
CC Q96E11; Q9H9D4: ZNF408; NbExp=3; IntAct=EBI-2855755, EBI-347633;
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=8;
CC Name=1;
CC IsoId=Q96E11-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96E11-2; Sequence=VSP_013489, VSP_013490;
CC Name=3;
CC IsoId=Q96E11-3; Sequence=VSP_013483;
CC Name=4;
CC IsoId=Q96E11-4; Sequence=VSP_013484;
CC Name=5;
CC IsoId=Q96E11-5; Sequence=VSP_013486, VSP_013487;
CC Name=6;
CC IsoId=Q96E11-6; Sequence=VSP_013485, VSP_013488;
CC Name=7;
CC IsoId=Q96E11-7; Sequence=VSP_013483, VSP_013485, VSP_013488;
CC Name=8;
CC IsoId=Q96E11-8; Sequence=VSP_042019;
CC -!- SIMILARITY: Belongs to the RRF family. {ECO:0000305}.
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DR EMBL; AK291603; BAF84292.1; -; mRNA.
DR EMBL; AK298046; BAH12711.1; -; mRNA.
DR EMBL; AK291809; BAF84498.1; -; mRNA.
DR EMBL; AK292790; BAF85479.1; -; mRNA.
DR EMBL; AK300718; BAH13333.1; -; mRNA.
DR EMBL; AK316266; BAH14637.1; -; mRNA.
DR EMBL; AL162424; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471090; EAW87525.1; -; Genomic_DNA.
DR EMBL; BC013049; AAH13049.1; -; mRNA.
DR EMBL; BC053604; AAH53604.1; -; mRNA.
DR CCDS; CCDS48013.1; -. [Q96E11-2]
DR CCDS; CCDS55336.1; -. [Q96E11-8]
DR CCDS; CCDS6840.1; -. [Q96E11-1]
DR CCDS; CCDS87684.1; -. [Q96E11-3]
DR RefSeq; NP_001166983.1; NM_001173512.2. [Q96E11-8]
DR RefSeq; NP_001333270.1; NM_001346341.1. [Q96E11-1]
DR RefSeq; NP_001333272.1; NM_001346343.1. [Q96E11-3]
DR RefSeq; NP_620132.1; NM_138777.4. [Q96E11-1]
DR RefSeq; NP_954646.1; NM_199177.3. [Q96E11-2]
DR PDB; 6NU2; EM; 3.90 A; z=59-262.
DR PDB; 6ZS9; EM; 4.00 A; A5=71-262.
DR PDB; 7L08; EM; 3.49 A; z=59-262.
DR PDB; 7L20; EM; 3.15 A; z=59-262.
DR PDB; 7NSH; EM; 3.20 A; BG=26-262.
DR PDB; 7NSI; EM; 4.60 A; BG=26-262.
DR PDBsum; 6NU2; -.
DR PDBsum; 6ZS9; -.
DR PDBsum; 7L08; -.
DR PDBsum; 7L20; -.
DR PDBsum; 7NSH; -.
DR PDBsum; 7NSI; -.
DR AlphaFoldDB; Q96E11; -.
DR SMR; Q96E11; -.
DR BioGRID; 124944; 322.
DR IntAct; Q96E11; 37.
DR MINT; Q96E11; -.
DR STRING; 9606.ENSP00000343867; -.
DR GlyGen; Q96E11; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q96E11; -.
DR MetOSite; Q96E11; -.
DR PhosphoSitePlus; Q96E11; -.
DR BioMuta; MRRF; -.
DR DMDM; 62901043; -.
DR EPD; Q96E11; -.
DR jPOST; Q96E11; -.
DR MassIVE; Q96E11; -.
DR MaxQB; Q96E11; -.
DR PaxDb; Q96E11; -.
DR PeptideAtlas; Q96E11; -.
DR PRIDE; Q96E11; -.
DR ProteomicsDB; 76354; -. [Q96E11-1]
DR ProteomicsDB; 76355; -. [Q96E11-2]
DR ProteomicsDB; 76356; -. [Q96E11-3]
DR ProteomicsDB; 76357; -. [Q96E11-4]
DR ProteomicsDB; 76358; -. [Q96E11-5]
DR ProteomicsDB; 76359; -. [Q96E11-6]
DR ProteomicsDB; 76360; -. [Q96E11-7]
DR ProteomicsDB; 76361; -. [Q96E11-8]
DR TopDownProteomics; Q96E11-3; -. [Q96E11-3]
DR Antibodypedia; 30275; 298 antibodies from 26 providers.
DR DNASU; 92399; -.
DR Ensembl; ENST00000297908.7; ENSP00000297908.3; ENSG00000148187.18. [Q96E11-8]
DR Ensembl; ENST00000344641.8; ENSP00000343867.3; ENSG00000148187.18. [Q96E11-1]
DR Ensembl; ENST00000373723.9; ENSP00000362828.4; ENSG00000148187.18. [Q96E11-2]
DR Ensembl; ENST00000373729.5; ENSP00000362834.1; ENSG00000148187.18. [Q96E11-3]
DR Ensembl; ENST00000394315.3; ENSP00000377850.3; ENSG00000148187.18. [Q96E11-2]
DR Ensembl; ENST00000467864.5; ENSP00000433288.1; ENSG00000148187.18. [Q96E11-4]
DR Ensembl; ENST00000470366.5; ENSP00000434807.1; ENSG00000148187.18. [Q96E11-6]
DR Ensembl; ENST00000489572.5; ENSP00000436360.1; ENSG00000148187.18. [Q96E11-6]
DR GeneID; 92399; -.
DR KEGG; hsa:92399; -.
DR MANE-Select; ENST00000344641.8; ENSP00000343867.3; NM_138777.5; NP_620132.1.
DR UCSC; uc010mwa.4; human. [Q96E11-1]
DR CTD; 92399; -.
DR DisGeNET; 92399; -.
DR GeneCards; MRRF; -.
DR HGNC; HGNC:7234; MRRF.
DR HPA; ENSG00000148187; Low tissue specificity.
DR MIM; 604602; gene.
DR neXtProt; NX_Q96E11; -.
DR OpenTargets; ENSG00000148187; -.
DR PharmGKB; PA31029; -.
DR VEuPathDB; HostDB:ENSG00000148187; -.
DR eggNOG; KOG4759; Eukaryota.
DR GeneTree; ENSGT00390000005084; -.
DR HOGENOM; CLU_073981_4_1_1; -.
DR InParanoid; Q96E11; -.
DR OMA; FNPMNNG; -.
DR OrthoDB; 1472838at2759; -.
DR PhylomeDB; Q96E11; -.
DR TreeFam; TF323691; -.
DR PathwayCommons; Q96E11; -.
DR Reactome; R-HSA-5419276; Mitochondrial translation termination.
DR SignaLink; Q96E11; -.
DR BioGRID-ORCS; 92399; 28 hits in 1046 CRISPR screens.
DR ChiTaRS; MRRF; human.
DR GenomeRNAi; 92399; -.
DR Pharos; Q96E11; Tbio.
DR PRO; PR:Q96E11; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q96E11; protein.
DR Bgee; ENSG00000148187; Expressed in rectum and 146 other tissues.
DR ExpressionAtlas; Q96E11; baseline and differential.
DR Genevisible; Q96E11; HS.
DR GO; GO:0005759; C:mitochondrial matrix; TAS:Reactome.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0043023; F:ribosomal large subunit binding; IBA:GO_Central.
DR GO; GO:0032790; P:ribosome disassembly; IDA:UniProtKB.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR Gene3D; 1.10.132.20; -; 1.
DR InterPro; IPR002661; Ribosome_recyc_fac.
DR InterPro; IPR023584; Ribosome_recyc_fac_dom.
DR InterPro; IPR036191; RRF_sf.
DR PANTHER; PTHR20982; PTHR20982; 1.
DR Pfam; PF01765; RRF; 1.
DR SUPFAM; SSF55194; SSF55194; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Mitochondrion; Protein biosynthesis;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..55
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 56..262
FT /note="Ribosome-recycling factor, mitochondrial"
FT /id="PRO_0000031080"
FT VAR_SEQ 1..44
FT /note="Missing (in isoform 3 and isoform 7)"
FT /evidence="ECO:0000305"
FT /id="VSP_013483"
FT VAR_SEQ 62..113
FT /note="Missing (in isoform 8)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_042019"
FT VAR_SEQ 154..262
FT /note="CTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAK
FT DSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG
FT -> ELAWIRSLRVPGIRSHLYHQLAV (in isoform 4)"
FT /evidence="ECO:0000305"
FT /id="VSP_013484"
FT VAR_SEQ 154..168
FT /note="CTAAAIKAIRESGMN -> TMKQIKQYFNGPNVA (in isoform 6 and
FT isoform 7)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_013485"
FT VAR_SEQ 154..158
FT /note="CTAAA -> VRWPC (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_013486"
FT VAR_SEQ 159..262
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_013487"
FT VAR_SEQ 169..262
FT /note="Missing (in isoform 6 and isoform 7)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_013488"
FT VAR_SEQ 185..201
FT /note="VTREHREMLVKLAKQNT -> SAKWPMTQWQNWTGIWQ (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_013489"
FT VAR_SEQ 202..262
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_013490"
FT VARIANT 216
FT /note="M -> V (in dbSNP:rs2297483)"
FT /id="VAR_051885"
SQ SEQUENCE 262 AA; 29277 MW; A4343465B5C9F5DE CRC64;
MALGLKCFRM VHPTFRNYLA ASIRPVSEVT LKTVHERQHG HRQYMAYSAV PVRHFATKKA
KAKGKGQSQT RVNINAALVE DIINLEEVNE EMKSVIEALK DNFNKTLNIR TSPGSLDKIA
VVTADGKLAL NQISQISMKS PQLILVNMAS FPECTAAAIK AIRESGMNLN PEVEGTLIRV
PIPQVTREHR EMLVKLAKQN TNKAKDSLRK VRTNSMNKLK KSKDTVSEDT IRLIEKQISQ
MADDTVAELD RHLAVKTKEL LG
