RRFM_MOUSE
ID RRFM_MOUSE Reviewed; 262 AA.
AC Q9D6S7; B1B055;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Ribosome-recycling factor, mitochondrial;
DE Short=RRF;
DE AltName: Full=Ribosome-releasing factor, mitochondrial;
DE Flags: Precursor;
GN Name=Mrrf;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Olfactory bulb, and Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP STRUCTURE BY NMR OF 116-186.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of ribosome recycling factor.";
RL Submitted (NOV-2004) to the PDB data bank.
CC -!- FUNCTION: Responsible for the release of ribosomes from messenger RNA
CC at the termination of protein biosynthesis. May increase the efficiency
CC of translation by recycling ribosomes from one round of translation to
CC another (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the RRF family. {ECO:0000305}.
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DR EMBL; AK010018; BAB26646.1; -; mRNA.
DR EMBL; AK032371; BAC27839.1; -; mRNA.
DR EMBL; BX293557; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC019787; AAH19787.1; -; mRNA.
DR CCDS; CCDS38114.1; -.
DR RefSeq; NP_080698.1; NM_026422.2.
DR PDB; 1WIH; NMR; -; A=116-186.
DR PDBsum; 1WIH; -.
DR AlphaFoldDB; Q9D6S7; -.
DR SMR; Q9D6S7; -.
DR BioGRID; 212498; 4.
DR IntAct; Q9D6S7; 1.
DR MINT; Q9D6S7; -.
DR STRING; 10090.ENSMUSP00000028250; -.
DR iPTMnet; Q9D6S7; -.
DR PhosphoSitePlus; Q9D6S7; -.
DR EPD; Q9D6S7; -.
DR MaxQB; Q9D6S7; -.
DR PaxDb; Q9D6S7; -.
DR PeptideAtlas; Q9D6S7; -.
DR PRIDE; Q9D6S7; -.
DR ProteomicsDB; 299897; -.
DR Antibodypedia; 30275; 298 antibodies from 26 providers.
DR Ensembl; ENSMUST00000028250; ENSMUSP00000028250; ENSMUSG00000026887.
DR GeneID; 67871; -.
DR KEGG; mmu:67871; -.
DR UCSC; uc008jlj.2; mouse.
DR CTD; 92399; -.
DR MGI; MGI:1915121; Mrrf.
DR VEuPathDB; HostDB:ENSMUSG00000026887; -.
DR eggNOG; KOG4759; Eukaryota.
DR GeneTree; ENSGT00390000005084; -.
DR HOGENOM; CLU_073981_4_1_1; -.
DR InParanoid; Q9D6S7; -.
DR OMA; FNPMNNG; -.
DR OrthoDB; 1472838at2759; -.
DR PhylomeDB; Q9D6S7; -.
DR TreeFam; TF323691; -.
DR Reactome; R-MMU-5419276; Mitochondrial translation termination.
DR BioGRID-ORCS; 67871; 12 hits in 73 CRISPR screens.
DR ChiTaRS; Mrrf; mouse.
DR EvolutionaryTrace; Q9D6S7; -.
DR PRO; PR:Q9D6S7; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9D6S7; protein.
DR Bgee; ENSMUSG00000026887; Expressed in embryonic post-anal tail and 223 other tissues.
DR ExpressionAtlas; Q9D6S7; baseline and differential.
DR Genevisible; Q9D6S7; MM.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0043023; F:ribosomal large subunit binding; IBA:GO_Central.
DR GO; GO:0032790; P:ribosome disassembly; ISO:MGI.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR Gene3D; 1.10.132.20; -; 1.
DR InterPro; IPR002661; Ribosome_recyc_fac.
DR InterPro; IPR023584; Ribosome_recyc_fac_dom.
DR InterPro; IPR036191; RRF_sf.
DR PANTHER; PTHR20982; PTHR20982; 1.
DR Pfam; PF01765; RRF; 1.
DR SUPFAM; SSF55194; SSF55194; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Mitochondrion; Protein biosynthesis; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..55
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 56..262
FT /note="Ribosome-recycling factor, mitochondrial"
FT /id="PRO_0000031081"
FT STRAND 120..123
FT /evidence="ECO:0007829|PDB:1WIH"
FT STRAND 126..129
FT /evidence="ECO:0007829|PDB:1WIH"
FT HELIX 130..133
FT /evidence="ECO:0007829|PDB:1WIH"
FT STRAND 134..140
FT /evidence="ECO:0007829|PDB:1WIH"
FT STRAND 143..147
FT /evidence="ECO:0007829|PDB:1WIH"
FT HELIX 155..163
FT /evidence="ECO:0007829|PDB:1WIH"
FT TURN 164..166
FT /evidence="ECO:0007829|PDB:1WIH"
FT STRAND 172..174
FT /evidence="ECO:0007829|PDB:1WIH"
FT STRAND 177..181
FT /evidence="ECO:0007829|PDB:1WIH"
SQ SEQUENCE 262 AA; 29051 MW; 52C3315B0886C850 CRC64;
MASGIRCFRL LHPAFRSYHA ALTRPVSEVS MKTVSGRQHG HRQYSAYPAV PVRHFATKKA
KAKGKGQPQA RVTVNRALVE DIISLEEVDE DMKSVVEALK DNFNKTLNIR TAPGSLDHIT
VVTADGKVAL NQIGQISMKS PQVILVNMAS FPECTAAAIK AIRESGMNLN PEVEGTLIRV
PIPKVTREHR EMLVKLAKQN TNKAKENLRK VRTNAMNKLK KSKDKTSEDT IRLIEKQISQ
MADDTVAELD QHLAAKTKEL LG
