AB8B_ARATH
ID AB8B_ARATH Reviewed; 1241 AA.
AC Q9LHK4;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Putative ABC transporter B family member 8;
DE Short=ABC transporter ABCB.8;
DE Short=AtABCB8;
DE AltName: Full=P-glycoprotein 8;
DE AltName: Full=Putative multidrug resistance protein 22;
GN Name=ABCB8; Synonyms=MDR22, PGP8; OrderedLocusNames=At3g30875;
GN ORFNames=MJI6.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Nakamura Y.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. III.";
RL Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11346655; DOI=10.1074/jbc.m103104200;
RA Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A.;
RT "The Arabidopsis thaliana ABC protein superfamily, a complete inventory.";
RL J. Biol. Chem. 276:30231-30244(2001).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18299247; DOI=10.1016/j.tplants.2008.02.001;
RA Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M.,
RA Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A.,
RA Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L.;
RT "Plant ABC proteins - a unified nomenclature and updated inventory.";
RL Trends Plant Sci. 13:151-159(2008).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255|PROSITE-ProRule:PRU00441};
CC Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. ABCB family.
CC Multidrug resistance exporter (TC 3.A.1.201) subfamily. {ECO:0000305}.
CC -!- CAUTION: Could be the product of a pseudogene. {ECO:0000305}.
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DR EMBL; AP002043; BAB02613.1; -; Genomic_DNA.
DR EMBL; CP002686; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; Q9LHK4; -.
DR SMR; Q9LHK4; -.
DR PeptideAtlas; Q9LHK4; -.
DR PRIDE; Q9LHK4; -.
DR Araport; AT3G30875; -.
DR InParanoid; Q9LHK4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LHK4; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0140359; F:ABC-type transporter activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0042626; F:ATPase-coupled transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1560.10; -; 2.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011527; ABC1_TM_dom.
DR InterPro; IPR036640; ABC1_TM_sf.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR039421; Type_1_exporter.
DR PANTHER; PTHR24221; PTHR24221; 1.
DR Pfam; PF00664; ABC_membrane; 2.
DR Pfam; PF00005; ABC_tran; 2.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR SUPFAM; SSF90123; SSF90123; 2.
DR PROSITE; PS50929; ABC_TM1F; 2.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE 5: Uncertain;
KW ATP-binding; Glycoprotein; Membrane; Nucleotide-binding;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1241
FT /note="Putative ABC transporter B family member 8"
FT /id="PRO_0000227933"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 263..283
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 686..706
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 716..736
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 797..815
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 821..838
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 899..919
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 933..953
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 33..323
FT /note="ABC transmembrane type-1 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 360..596
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT DOMAIN 676..964
FT /note="ABC transmembrane type-1 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 998..1236
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 395..402
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 1033..1040
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT CARBOHYD 48
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 571
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 632
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 648
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 773
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 855
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1187
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1241 AA; 137112 MW; DD3367D1E7F07D28 CRC64;
MRSQAKTETV SSKSSRNTHV IFRFADWIDI VLMVLGSVGA IGDGMSTNVS LVFVSRIMNT
LGYSQHNPSS TNFKEEIQKC SLYFVYLGLA ILGVAFMEGY CWSKTSERQV MKIRRTYLEA
VLRQEVSFFD SDISTSEIIH TISTDTSLIQ QLLSEKVPIF LMHISVFITG LVFSAYFSWR
LTVVAIPTLV LLLIPGLIYG KYLVHLSKKS FKEYTKANSI VEQALSSIKT ILSFTAETQI
IKKYSEVLER HKKLGLKQGL AKGLAVGSSG ISFTIWAFLA WYGSRLVMHK QETGGRIYAA
GISFVLGGIS LGTALTEIRY FSEASVAAAR ICSRIDRISE IDGEDTKKGF IPGEKMKGRV
EFERVTLVYL SRPETIILKD FTLTVDVGQS VALMGASGSG KSTVIALLQR FYDPCEGFVR
IDGFDIKTLQ LKWMRQHIGV VSQDHALFGT SIMENLMFGK NKASMDEVIS AAKAANAHGF
ITQLPNGYDT HIGNRGALLS GGQKQRIAIA RAIIRNPVIL LLDEATSALD GESETLIQNA
LDQVAAGRTT LVVAHKLSTV RGANIIAMLE NGSVRELGSH EDLMTKNNHY AKLVKLQRQF
GHEHQQDLQD RVNSPEIQQR WSTMNSVIRL SNRSSPDLIV SPITLESNHT TKINENIPST
SFTRLLPFVS PEWKSSLVGC ISATTFGAIQ PVYALSIGGM ISAFFAKSSQ EMQDKIHIYS
LIFISLTFLS ITLNLLQHYS FAKMGERLMQ RLRLKMLEKI FTFEPAWFDV EENFTSEICS
RLNNEVSIVK SLVADRISLL VQTISGVTIA MIIGLLISWK LALVMIAVQP LSILCFYTKK
VLLSKISNNY AYAQNRSSQI ASEAIYNHKI VTSLGSTKKI IEIFDNAQYE AKRKGRKAAW
LAGFGMGSAQ CLTFLTWALD FWYGGVLVQK GEISAGDVFK TFFVLVSTGK VIAEAGSMTS
DLAKGTAAIS SVFNILDRPS SHENTNHGEK MGTIQGRIEL KNIDFSYPNR PSILVLRDFS
LDIKPGTSIG LVGTSGCGKS TVIALIQRFY DVEIGCVKID SENLRDINIK WYRKHTALVS
QEPVVYSGSI QDNIILGRPE ATEDEVVEAA KAANAHDFIS AMEKGYKTEC GERGVQLSGG
QKQRIAIARA FLRSPIILLL DEVTSSLDSN SEQEVQDALA RIMASRNMTT VVVAHRLNTL
KNLDCIALIV DGTVIETGSY DHLKNIGGQF SRLAHAHDLK S
