RRF_BACSU
ID RRF_BACSU Reviewed; 185 AA.
AC P81101; O31750;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Ribosome-recycling factor {ECO:0000255|HAMAP-Rule:MF_00040};
DE Short=RRF {ECO:0000255|HAMAP-Rule:MF_00040};
DE AltName: Full=Ribosome-releasing factor {ECO:0000255|HAMAP-Rule:MF_00040};
DE AltName: Full=Vegetative protein 12B;
DE Short=VEG12B;
GN Name=frr {ECO:0000255|HAMAP-Rule:MF_00040}; OrderedLocusNames=BSU16520;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [2]
RP PROTEIN SEQUENCE OF 2-26.
RC STRAIN=168 / JH642;
RA Graumann P.L., Schmid R., Marahiel M.A.;
RL Submitted (OCT-1997) to UniProtKB.
RN [3]
RP CHARACTERIZATION.
RC STRAIN=168 / JH642;
RX PubMed=8755892; DOI=10.1128/jb.178.15.4611-4619.1996;
RA Graumann P., Schroeder K., Schmid R., Marahiel M.A.;
RT "Cold shock stress-induced proteins in Bacillus subtilis.";
RL J. Bacteriol. 178:4611-4619(1996).
RN [4]
RP DISRUPTION PHENOTYPE, AND FUNCTION IN SPORULATION.
RC STRAIN=168;
RX PubMed=14586115; DOI=10.1271/bbb.67.2245;
RA Ohashi Y., Inaoka T., Kasai K., Ito Y., Okamoto S., Satsu H., Tozawa Y.,
RA Kawamura F., Ochi K.;
RT "Expression profiling of translation-associated genes in sporulating
RT Bacillus subtilis and consequence of sporulation by gene inactivation.";
RL Biosci. Biotechnol. Biochem. 67:2245-2253(2003).
CC -!- FUNCTION: Responsible for the release of ribosomes from messenger RNA
CC at the termination of protein biosynthesis. May increase the efficiency
CC of translation by recycling ribosomes from one round of translation to
CC another. {ECO:0000255|HAMAP-Rule:MF_00040}.
CC -!- FUNCTION: Plays a role in sporulation. {ECO:0000269|PubMed:14586115}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- DISRUPTION PHENOTYPE: No sporulation at 37 or 47 degrees Celsius.
CC {ECO:0000269|PubMed:14586115}.
CC -!- SIMILARITY: Belongs to the RRF family. {ECO:0000255|HAMAP-
CC Rule:MF_00040}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL009126; CAB13525.1; -; Genomic_DNA.
DR PIR; G69626; G69626.
DR RefSeq; NP_389534.1; NC_000964.3.
DR RefSeq; WP_003231927.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; P81101; -.
DR SMR; P81101; -.
DR IntAct; P81101; 1.
DR MINT; P81101; -.
DR STRING; 224308.BSU16520; -.
DR jPOST; P81101; -.
DR PaxDb; P81101; -.
DR PRIDE; P81101; -.
DR EnsemblBacteria; CAB13525; CAB13525; BSU_16520.
DR GeneID; 939605; -.
DR KEGG; bsu:BSU16520; -.
DR PATRIC; fig|224308.179.peg.1793; -.
DR eggNOG; COG0233; Bacteria.
DR InParanoid; P81101; -.
DR OMA; FNPMNNG; -.
DR PhylomeDB; P81101; -.
DR BioCyc; BSUB:BSU16520-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0043023; F:ribosomal large subunit binding; IBA:GO_Central.
DR GO; GO:0002184; P:cytoplasmic translational termination; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR CDD; cd00520; RRF; 1.
DR Gene3D; 1.10.132.20; -; 1.
DR HAMAP; MF_00040; RRF; 1.
DR InterPro; IPR002661; Ribosome_recyc_fac.
DR InterPro; IPR023584; Ribosome_recyc_fac_dom.
DR InterPro; IPR036191; RRF_sf.
DR PANTHER; PTHR20982; PTHR20982; 1.
DR Pfam; PF01765; RRF; 1.
DR SUPFAM; SSF55194; SSF55194; 1.
DR TIGRFAMs; TIGR00496; frr; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; Protein biosynthesis;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|Ref.2"
FT CHAIN 2..185
FT /note="Ribosome-recycling factor"
FT /id="PRO_0000167412"
FT CONFLICT 26
FT /note="T -> D (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 185 AA; 20767 MW; 40BDBB6ED1EC412C CRC64;
MSKEVLTQTK EKMEKAIAAY QRELATVRAG RANPSLLDKV TVEYYGAQTP LNQLSSINVP
EARMLVITPY DKTAIGDIEK AILKADLGLT PTSDGNMIRI AIPALTEERR KELVKVVKKY
AEEAKVAVRN VRRDANDDLK KLEKNGDITE DELRASTEDV QKLTDEYVSK IDSVTKDKEK
EIMEV
