AB8C_ARATH
ID AB8C_ARATH Reviewed; 1464 AA.
AC Q8LGU1; Q9LU34;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 3.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=ABC transporter C family member 8;
DE Short=ABC transporter ABCC.8;
DE Short=AtABCC8;
DE EC=7.6.2.2;
DE AltName: Full=ATP-energized glutathione S-conjugate pump 6;
DE AltName: Full=Glutathione S-conjugate-transporting ATPase 6;
DE AltName: Full=Multidrug resistance-associated protein 6;
DE Flags: Precursor;
GN Name=ABCC8; Synonyms=MRP6, MRP8; OrderedLocusNames=At3g21250;
GN ORFNames=MXL8.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 79-1453 (ISOFORM 2), AND TISSUE SPECIFICITY.
RX PubMed=12430019; DOI=10.1007/s00425-002-0890-6;
RA Kolukisaoglu U.H., Bovet L., Klein M., Eggmann T., Geisler M., Wanke D.,
RA Martinoia E., Schulz B.;
RT "Family business: the multidrug-resistance related protein (MRP) ABC
RT transporter genes in Arabidopsis thaliana.";
RL Planta 216:107-119(2002).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11346655; DOI=10.1074/jbc.m103104200;
RA Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A.;
RT "The Arabidopsis thaliana ABC protein superfamily, a complete inventory.";
RL J. Biol. Chem. 276:30231-30244(2001).
RN [5]
RP GENE FAMILY.
RX PubMed=11855639; DOI=10.1007/s004250100661;
RA Martinoia E., Klein M., Geisler M., Bovet L., Forestier C.,
RA Kolukisaoglu H.U., Mueller-Roeber B., Schulz B.;
RT "Multifunctionality of plant ABC transporters -- more than just
RT detoxifiers.";
RL Planta 214:345-355(2002).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18299247; DOI=10.1016/j.tplants.2008.02.001;
RA Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M.,
RA Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A.,
RA Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L.;
RT "Plant ABC proteins - a unified nomenclature and updated inventory.";
RL Trends Plant Sci. 13:151-159(2008).
CC -!- FUNCTION: Pump for glutathione S-conjugates. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + xenobioticSide 1 = ADP + phosphate +
CC xenobioticSide 2.; EC=7.6.2.2;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255|PROSITE-ProRule:PRU00441};
CC Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8LGU1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8LGU1-2; Sequence=VSP_041303;
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:12430019}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. ABCC family.
CC Conjugate transporter (TC 3.A.1.208) subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB01717.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAD44995.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB023045; BAB01717.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE76481.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76482.1; -; Genomic_DNA.
DR EMBL; AJ507057; CAD44995.1; ALT_INIT; mRNA.
DR RefSeq; NP_001189944.1; NM_001203015.2. [Q8LGU1-1]
DR RefSeq; NP_188762.3; NM_113020.3. [Q8LGU1-2]
DR AlphaFoldDB; Q8LGU1; -.
DR SMR; Q8LGU1; -.
DR STRING; 3702.AT3G21250.2; -.
DR iPTMnet; Q8LGU1; -.
DR PaxDb; Q8LGU1; -.
DR PRIDE; Q8LGU1; -.
DR ProteomicsDB; 244391; -. [Q8LGU1-1]
DR EnsemblPlants; AT3G21250.1; AT3G21250.1; AT3G21250. [Q8LGU1-2]
DR EnsemblPlants; AT3G21250.2; AT3G21250.2; AT3G21250. [Q8LGU1-1]
DR GeneID; 821679; -.
DR Gramene; AT3G21250.1; AT3G21250.1; AT3G21250. [Q8LGU1-2]
DR Gramene; AT3G21250.2; AT3G21250.2; AT3G21250. [Q8LGU1-1]
DR KEGG; ath:AT3G21250; -.
DR Araport; AT3G21250; -.
DR TAIR; locus:2094721; AT3G21250.
DR eggNOG; KOG0054; Eukaryota.
DR HOGENOM; CLU_000604_27_0_1; -.
DR InParanoid; Q8LGU1; -.
DR PRO; PR:Q8LGU1; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8LGU1; baseline and differential.
DR Genevisible; Q8LGU1; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005774; C:vacuolar membrane; HDA:TAIR.
DR GO; GO:0008559; F:ABC-type xenobiotic transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0042626; F:ATPase-coupled transmembrane transporter activity; ISS:TAIR.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR CDD; cd18579; ABC_6TM_ABCC_D1; 1.
DR CDD; cd18580; ABC_6TM_ABCC_D2; 1.
DR Gene3D; 1.20.1560.10; -; 2.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011527; ABC1_TM_dom.
DR InterPro; IPR036640; ABC1_TM_sf.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR044746; ABCC_6TM_D1.
DR InterPro; IPR044726; ABCC_6TM_D2.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00664; ABC_membrane; 2.
DR Pfam; PF00005; ABC_tran; 2.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR SUPFAM; SSF90123; SSF90123; 2.
DR PROSITE; PS50929; ABC_TM1F; 2.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Membrane; Nucleotide-binding;
KW Reference proteome; Repeat; Signal; Translocase; Transmembrane;
KW Transmembrane helix; Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..1464
FT /note="ABC transporter C family member 8"
FT /id="PRO_0000226077"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 321..343
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 391..411
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 508..528
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 909..929
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 931..951
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 1008..1028
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 1030..1050
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 1132..1152
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 282..564
FT /note="ABC transmembrane type-1 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 600..821
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT DOMAIN 901..1178
FT /note="ABC transmembrane type-1 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 1215..1449
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 633..640
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 1249..1256
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT VAR_SEQ 782..792
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12430019"
FT /id="VSP_041303"
FT CONFLICT 350
FT /note="M -> L (in Ref. 3; CAD44995)"
FT /evidence="ECO:0000305"
FT CONFLICT 1301
FT /note="L -> F (in Ref. 3; CAD44995)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1464 AA; 162072 MW; E446F0F714FB4BDB CRC64;
MAAFIGSLPW CDVELNLASS CFQRTAIAFV NLLFLCIFYL FLIASCVSTH FIVRGRKKGW
IFVAVAICCA ITSFIFLGVG LNSLIHGGND VTEISWVACF VEGIIWVSLA VSLLVNGSKW
VNILVSVWWV SFALLDLVAK SGILLQGNGI RILDILTLPM SLLLLLCSWM NLRSSSAAAQ
DCSVTGLSDP LLTKNPRKES ARLATAGFFS ILSFSWMNPL LSLGFKKPLS PEDIPSVVPE
DEAQLAYKKF SQAWDTLLGD ESSTKERNLV FRAVVKVYFK ENIFIAVFAF LRTFAVVSLP
LMLYVFVDYA NSDHRDLRNG FFNLACLVML KLVESLTMRH WYFASRRSGM RIRSALMVAA
YKKQLKLSSL GRKRHSSGEI VNYIAVDAYR MGEFLWWFHS GWSLSLQLLL STAVLFGVVG
AGAFPGLILL LLCGLLNLPF AKMLQNCQTQ FMIAQDKRLR STSEILNSMK VIKLQSWEDE
FKKKIESCRD DEFTWLAKAQ LTKAFGSFLY WMSPTIVSSV VFLGCALLKS APLNASTIFT
VLATLRVMSE PVKIIPDAIS AIIQGNVSFQ RLNNFLLDDE LKMDEIERSG LDASGTAVDI
QVGNFGWEPE TKIPTLRNIH LEIKHGQKVA VCGPVGAGKS SLLHAVLGEI PKVSGTVKVF
GSIAYVSQTS WIQSGTIRDN ILYGKPMESR RYNAAIKACA LDKDMNGFGH GDLTEIGQRG
INLSGGQKQR IQLARAVYAD ADVYLLDDPF SAVDAHTAGV LFHKCVEDSL KEKTVILVTH
QVEFLSEVDQ ILVMEEGTIT QSGKYEELLM MGTAFQQLVN AHNDAVTVLP LASNESLGDL
RKEGKDREIR NMTVVEKIEE EIEKTDIPGV QLTQEEEKES GYVGMKPFLD YIGVSRGWCL
LWSSVLGQVG FVVFQAASTY WLAFAIGIPK ITNTMLIGVY SIISTLSAGF VYARAITTAH
LGLKASKAFF SGFTNAVFKA PMLFFDSTPV GRILTRASSD LNVLDYDVPF AFIFVVAPAV
ELTAALLIMT YVTWQVIIIA LLALAATKVV QDYYLASARE LIRINGTTKA PVMNYAAETS
LGVVTIRAFG TAERFFKNYL NLVDADAVLF FLSNAAMEWV ILRIETLQNV TLFTCALLLI
LIPKGYIAPG LVGLSLSYAL TLTQTQVFLT RWYCTLSNSI ISVERIKQYM NIPEEPPAII
DDKRPPSSWP SNGTIHLQEL KIRYRPNAPL VLKGISCTFR EGTRVGVVGR TGSGKSTLIS
ALFRLVEPAS GCILIDGIDI SKIGLKDLRM KLSIIPQEPT LFRGCIRTNL DPLGVYSDDE
IWKALEKCQL KTTISNLPNK LDSSVSDEGE NWSVGQRQLF CLGRVLLKRN KILVLDEATA
SIDSATDAII QRIIREEFAD CTVITVAHRV PTVIDSDMVM VLSFGDLVEY NEPSKLMETD
SYFSKLVAEY WASCRGNSSQ NLQL
