AVR10_PHYIT
ID AVR10_PHYIT Reviewed; 135 AA.
AC D0NIW0;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 1.
DT 03-AUG-2022, entry version 34.
DE RecName: Full=RxLR effector protein Avr10 {ECO:0000303|PubMed:30329083};
DE AltName: Full=Avirulence protein 10 {ECO:0000303|PubMed:30329083};
DE Flags: Precursor;
GN Name=Avr10 {ECO:0000303|PubMed:30329083}; ORFNames=PITG_11484;
OS Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=403677;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC STRAIN=T30-4;
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=11768540; DOI=10.1094/mpmi.2001.14.12.1444;
RA van der Lee T., Testa A., van 't Klooster J., van den Berg-Velthuis G.,
RA Govers F.;
RT "Chromosomal deletion in isolates of Phytophthora infestans correlates with
RT virulence on R3, R10, and R11 potato lines.";
RL Mol. Plant Microbe Interact. 14:1444-1452(2001).
RN [3]
RP INDUCTION.
RX PubMed=28228125; DOI=10.1186/s12864-017-3585-x;
RA Ah-Fong A.M., Kim K.S., Judelson H.S.;
RT "RNA-seq of life stages of the oomycete Phytophthora infestans reveals
RT dynamic changes in metabolic, signal transduction, and pathogenesis genes
RT and a major role for calcium signaling in development.";
RL BMC Genomics 18:198-198(2017).
RN [4]
RP INDUCTION, AND DOMAIN.
RX PubMed=29312401; DOI=10.3389/fpls.2017.02155;
RA Yin J., Gu B., Huang G., Tian Y., Quan J., Lindqvist-Kreuze H., Shan W.;
RT "Conserved RXLR effector genes of Phytophthora infestans expressed at the
RT early stage of potato infection are suppressive to host defense.";
RL Front. Plant Sci. 8:2155-2155(2017).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=30329083; DOI=10.1093/jxb/ery360;
RA Wang S., McLellan H., Bukharova T., He Q., Murphy F., Shi J., Sun S.,
RA van Weymers P., Ren Y., Thilliez G., Wang H., Chen X., Engelhardt S.,
RA Vleeshouwers V., Gilroy E.M., Whisson S.C., Hein I., Wang X., Tian Z.,
RA Birch P.R.J., Boevink P.C.;
RT "Phytophthora infestans RXLR effectors act in concert at diverse
RT subcellular locations to enhance host colonization.";
RL J. Exp. Bot. 70:343-356(2019).
CC -!- FUNCTION: Secreted effector that acts as an elicitor of hypersensitive
CC response (HR) specifically on plants carrying defense protein R10
CC (PubMed:11768540). Enhances P.infestans colonization of Nicotiana
CC benthamiana leaves (PubMed:30329083). {ECO:0000269|PubMed:11768540,
CC ECO:0000269|PubMed:30329083}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30329083}. Host
CC nucleus {ECO:0000269|PubMed:30329083}. Host cytoplasm
CC {ECO:0000269|PubMed:30329083}.
CC -!- INDUCTION: Expression is induced during host plant infection.
CC {ECO:0000269|PubMed:19741609, ECO:0000269|PubMed:28228125,
CC ECO:0000269|PubMed:29312401}.
CC -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC phosphate. {ECO:0000305|PubMed:29312401}.
CC -!- DISRUPTION PHENOTYPE: Leads to gain of virulence on potato lines
CC carrying defense protein R10. {ECO:0000269|PubMed:11768540}.
CC -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR EMBL; DS028140; EEY59444.1; -; Genomic_DNA.
DR RefSeq; XP_002901054.1; XM_002901008.1.
DR AlphaFoldDB; D0NIW0; -.
DR EnsemblProtists; PITG_11484T0; PITG_11484T0; PITG_11484.
DR GeneID; 9474826; -.
DR KEGG; pif:PITG_11484; -.
DR VEuPathDB; FungiDB:PITG_11484; -.
DR eggNOG; ENOG502RGK4; Eukaryota.
DR HOGENOM; CLU_140782_0_0_1; -.
DR OMA; GTWYRHY; -.
DR OrthoDB; 1618786at2759; -.
DR Proteomes; UP000006643; Partially assembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
PE 2: Evidence at transcript level;
KW Host cytoplasm; Host nucleus; Reference proteome; Secreted; Signal;
KW Virulence.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..135
FT /note="RxLR effector protein Avr10"
FT /id="PRO_5003012475"
FT REGION 34..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 44..63
FT /note="RxLR-dEER"
FT /evidence="ECO:0000305|PubMed:29312401"
FT COMPBIAS 47..61
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 135 AA; 14982 MW; 3E4AFC79570E58CF CRC64;
MRLSFIIFAI SLLAGGSGAA EALHPASDVL TLRGTNQGAS TGKRSLRYDN NAERAGEEDD
EERAFPGAEE LSRLANLAHT SKADSLGTSL KNFFKQLDKA NVNPSNIHKY GFSGEEFDQL
RKRFGTWYRH YKDIE
