AVR4_PHYIT
ID AVR4_PHYIT Reviewed; 287 AA.
AC B1NNT7; D0N8A3;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 29.
DE RecName: Full=RxLR effector protein Avr4 {ECO:0000303|PubMed:30329083};
DE AltName: Full=Avirulence protein 4 {ECO:0000303|PubMed:30329083};
DE Flags: Precursor;
GN Name=Avr4 {ECO:0000303|PubMed:18842095}; ORFNames=PITG_07387;
OS Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=403677;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DOMAIN.
RC STRAIN=T30-4;
RX PubMed=18842095; DOI=10.1094/mpmi-21-11-1460;
RA van Poppel P.M., Guo J., van de Vondervoort P.J., Jung M.W., Birch P.R.,
RA Whisson S.C., Govers F.;
RT "The Phytophthora infestans avirulence gene Avr4 encodes an RXLR-dEER
RT effector.";
RL Mol. Plant Microbe Interact. 21:1460-1470(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Race 11;
RA Pankin A.A., Kinash E.A., Kozlovskaya I.N., Kuznetsova M.A., Khavkin E.E.;
RT "Are simple Phytophthora infestans races really that simple?";
RL PPO Spec. Rep. 15:205-211(2012).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC STRAIN=T30-4;
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND DOMAIN.
RX PubMed=19694952; DOI=10.1111/j.1364-3703.2009.00556.x;
RA Van Poppel P.M., Jiang R.H., Sliwka J., Govers F.;
RT "Recognition of Phytophthora infestans Avr4 by potato R4 is triggered by C-
RT terminal domains comprising W motifs.";
RL Mol. Plant Pathol. 10:611-620(2009).
RN [5]
RP INDUCTION.
RX PubMed=29312401; DOI=10.3389/fpls.2017.02155;
RA Yin J., Gu B., Huang G., Tian Y., Quan J., Lindqvist-Kreuze H., Shan W.;
RT "Conserved RXLR effector genes of Phytophthora infestans expressed at the
RT early stage of potato infection are suppressive to host defense.";
RL Front. Plant Sci. 8:2155-2155(2017).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=30329083; DOI=10.1093/jxb/ery360;
RA Wang S., McLellan H., Bukharova T., He Q., Murphy F., Shi J., Sun S.,
RA van Weymers P., Ren Y., Thilliez G., Wang H., Chen X., Engelhardt S.,
RA Vleeshouwers V., Gilroy E.M., Whisson S.C., Hein I., Wang X., Tian Z.,
RA Birch P.R.J., Boevink P.C.;
RT "Phytophthora infestans RXLR effectors act in concert at diverse
RT subcellular locations to enhance host colonization.";
RL J. Exp. Bot. 70:343-356(2019).
CC -!- FUNCTION: Secreted effector that acts as an elicitor of hypersensitive
CC response (HR) specifically on plants carrying defense protein R4,
CC through its interaction with this protein (PubMed:18842095,
CC PubMed:19694952). {ECO:0000269|PubMed:18842095,
CC ECO:0000269|PubMed:19694952}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:30329083}. Host
CC cytoplasm {ECO:0000269|PubMed:30329083}. Host nucleus
CC {ECO:0000269|PubMed:30329083}. Host nucleus, host nucleolus
CC {ECO:0000269|PubMed:30329083}. Host cytoplasm, host cytoskeleton
CC {ECO:0000269|PubMed:30329083}.
CC -!- INDUCTION: Expression is induced during host plant infection.
CC {ECO:0000269|PubMed:19741609, ECO:0000269|PubMed:29312401}.
CC -!- DOMAIN: Oomycete Avh family proteins have a modular structure with an
CC N-terminal signal peptide (SP), an RXLR-dEER domain and a C-terminus
CC with a variable number of modules that consist of W, Y and L motifs
CC after highly conserved residues (PubMed:19694952). The C-ter of Avr4
CC contains 3 W motifs, 1 Y motif but no L motif. The RXLR-dEER motif
CC functions as a host targeting signal (HTS) (PubMed:18842095). The
CC region containing the W2 motif, in combination with either the W1 or W3
CC motif, triggers a necrotic response in potato plants carrying the
CC resistance gene R4. {ECO:0000269|PubMed:18842095,
CC ECO:0000269|PubMed:19694952}.
CC -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR EMBL; EF672355; ABV66276.1; -; Genomic_DNA.
DR EMBL; KF188223; AGV54943.1; -; Genomic_DNA.
DR EMBL; DS028128; EEY53788.1; -; Genomic_DNA.
DR RefSeq; XP_002904419.1; XM_002904373.1.
DR AlphaFoldDB; B1NNT7; -.
DR EnsemblProtists; PITG_07387T0; PITG_07387T0; PITG_07387.
DR GeneID; 9480388; -.
DR KEGG; pif:PITG_07387; -.
DR VEuPathDB; FungiDB:PITG_07387; -.
DR eggNOG; ENOG502RGEU; Eukaryota.
DR HOGENOM; CLU_974769_0_0_1; -.
DR OMA; IISFGRD; -.
DR OrthoDB; 1408620at2759; -.
DR PHI-base; PHI:5098; -.
DR Proteomes; UP000006643; Partially assembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0044163; C:host cytoskeleton; IEA:UniProtKB-SubCell.
PE 2: Evidence at transcript level;
KW Host cytoplasm; Host cytoskeleton; Host nucleus; Reference proteome;
KW Repeat; Secreted; Signal; Virulence.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..287
FT /note="RxLR effector protein Avr4"
FT /id="PRO_5002769343"
FT REGION 115..138
FT /note="W1 motif"
FT /evidence="ECO:0000269|PubMed:19694952"
FT REGION 148..171
FT /note="W2 motif"
FT /evidence="ECO:0000269|PubMed:19694952"
FT REGION 221..244
FT /note="W3 motif"
FT /evidence="ECO:0000269|PubMed:19694952"
FT REGION 246..267
FT /note="Y motif"
FT /evidence="ECO:0000269|PubMed:19694952"
FT MOTIF 42..55
FT /note="RxLR-dEER"
FT /evidence="ECO:0000305|PubMed:18842095"
SQ SEQUENCE 287 AA; 33048 MW; 4518728F030F3FC2 CRC64;
MRSLHILLVF TASLLASLTE SAKADSLART VSVVDNVKVK SRFLRAQTDE KNEERATITL
GDRVVSDKAA TKDLLQQLLA LGTPLEKVQK QFLNIPQMKT FAELSKHPNW KALDKYERMQ
WQKLKEGETL TFMRLGDRLY SKEKAQEQLL RWVAQKKPVE SVYDDLQVAG FAHNTVAARQ
NWRAYIMYDK WFTAASQMQR NPQQYAKFGT GYHSEQKTTE LFEKWAMEGT HIKSVITTLK
LNGKSASEMA NNENFPALLK YVKLYLDFKP VRDLNAKSRL QARRPIS
