ID   AVR9_PASFU              Reviewed;          63 AA.
AC   P22287;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Race-specific elicitor A9;
DE   Flags: Precursor;
GN   Name=AVR9;
OS   Passalora fulva (Tomato leaf mold) (Cladosporium fulvum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Fulvia.
OX   NCBI_TaxID=5499;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Race 5;
RX   PubMed=1799694; DOI=10.1094/mpmi-4-052;
RA   van Kan J.A.L., van den Ackerveken G.F.J.M., de Wit P.J.G.M.;
RT   "Cloning and characterization of cDNA of avirulence gene avr9 of the fungal
RT   pathogen Cladosporium fulvum, causal agent of tomato leaf mold.";
RL   Mol. Plant Microbe Interact. 4:52-59(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Race 5;
RX   PubMed=1303800; DOI=10.1111/j.1365-313x.1992.00359.x;
RA   Van Den Ackerveken G.F.J.M., van Kan J.A.L., de Wit P.J.G.M.;
RT   "Molecular analysis of the avirulence gene avr9 of the fungal tomato
RT   pathogen Cladosporium fulvum fully supports the gene-for-gene hypothesis.";
RL   Plant J. 2:359-366(1992).
RN   [3]
RP   PROTEIN SEQUENCE OF 36-62.
RA   Scholtens-Tomas I.M.J., de Wit P.J.G.M.;
RT   "Purification and primary structure of a necrosis-inducing peptide from the
RT   apoplastic fluids of tomato infected with Cladosporium fulvum (syn. Fulvia
RT   fulva).";
RL   Physiol. Mol. Plant Pathol. 33:59-67(1988).
RN   [4]
RP   PROTEOLYTIC PROCESSING.
RX   PubMed=8208859; DOI=10.1104/pp.103.1.91;
RA   van den Ackerveken G.F.J.M., Vossen P., de Wit P.J.G.M.;
RT   "The AVR9 race-specific elicitor of Cladosporium fulvum is processed by
RT   endogenous and plant proteases.";
RL   Plant Physiol. 103:91-96(1993).
RN   [5]
RP   SYNTHESIS.
RX   PubMed=9924993; DOI=10.1111/j.1399-3011.1998.tb01253.x;
RA   Mahe E., Vossen P., Van den Hooven H.W., Le-Nguyen D., Vervoort J.,
RA   De Wit P.J.G.M.;
RT   "Solid-phase synthesis, conformational analysis, and biological activity of
RT   AVR9 elicitor peptides of the fungal tomato pathogen Cladosporium fulvum.";
RL   J. Pept. Res. 52:482-494(1998).
RN   [6]
RP   STRUCTURE BY NMR, AND DISULFIDE BONDS.
RX   PubMed=9119054; DOI=10.1016/s0014-5793(97)00117-8;
RA   Vervoort J., van den Hooven H.W., Berg A., Vossen P., Vogelsang R.,
RA   Joostwn M.H.A.J., de Wit P.J.G.M.;
RT   "The race-specific elicitor AVR9 of the tomato pathogen Cladosporium
RT   fulvum: a cystine knot protein. Sequence-specific 1H NMR assignments,
RT   secondary structure and global fold of the protein.";
RL   FEBS Lett. 404:153-158(1997).
CC   -!- FUNCTION: This necrosis-inducing peptide induces a hypersensitive
CC       response on Cf-9 tomato genotypes. Race-specific elicitors are
CC       compounds which only induce defense responses in genotypes of host
CC       plants which are resistant to the pathogenic race that produces the
CC       elicitor, but not in susceptible genotypes.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
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DR   EMBL; M55289; AAA03344.1; -; mRNA.
DR   EMBL; X60284; CAA42824.1; -; Genomic_DNA.
DR   PIR; JQ2276; JQ2276.
DR   AlphaFoldDB; P22287; -.
DR   KEGG; ag:AAA03344; -.
DR   PHI-base; PHI:2845; -.
DR   PHI-base; PHI:5473; -.
DR   PHI-base; PHI:5497; -.
DR   PHI-base; PHI:5542; -.
DR   PHI-base; PHI:5545; -.
DR   PHI-base; PHI:5558; -.
DR   PHI-base; PHI:5566; -.
DR   PHI-base; PHI:7; -.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Knottin; Signal; Virulence.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   PROPEP          24..35
FT                   /evidence="ECO:0000269|Ref.3"
FT                   /id="PRO_0000020769"
FT   PEPTIDE         36..63
FT                   /note="Race-specific elicitor A9"
FT                   /id="PRO_0000020770"
FT   DISULFID        37..51
FT                   /evidence="ECO:0000269|PubMed:9119054"
FT   DISULFID        41..54
FT                   /evidence="ECO:0000269|PubMed:9119054"
FT   DISULFID        47..61
FT                   /evidence="ECO:0000269|PubMed:9119054"
SQ   SEQUENCE   63 AA;  6741 MW;  E4E330FD68F6D5AA CRC64;
     MKLSLLSVEL ALLIATTLPL CWAAALPVGL GVGLDYCNSS CTRAFDCLGQ CGRCDFHKLQ
     CVH