AVT2_YEAST
ID AVT2_YEAST Reviewed; 480 AA.
AC P39981; D3DLI6; Q2VQX2;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Vacuolar amino acid transporter 2;
GN Name=AVT2; OrderedLocusNames=YEL064C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-94.
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=15905473; DOI=10.1093/nar/gki583;
RA Zhang Z., Dietrich F.S.;
RT "Mapping of transcription start sites in Saccharomyces cerevisiae using 5'
RT SAGE.";
RL Nucleic Acids Res. 33:2838-2851(2005).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11274162; DOI=10.1074/jbc.m008028200;
RA Russnak R., Konczal D., McIntire S.L.;
RT "A family of yeast proteins mediating bidirectional vacuolar amino acid
RT transport.";
RL J. Biol. Chem. 276:23849-23857(2001).
CC -!- FUNCTION: Probable amino acid transporter of unknown specificity.
CC {ECO:0000269|PubMed:11274162}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:11274162};
CC Multi-pass membrane protein {ECO:0000269|PubMed:11274162}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC {ECO:0000305}.
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DR EMBL; U18795; AAB65023.1; -; Genomic_DNA.
DR EMBL; AY899246; AAX83931.1; -; mRNA.
DR EMBL; BK006939; DAA07590.1; -; Genomic_DNA.
DR PIR; S50525; S50525.
DR RefSeq; NP_010850.1; NM_001178879.1.
DR AlphaFoldDB; P39981; -.
DR SMR; P39981; -.
DR BioGRID; 36665; 14.
DR DIP; DIP-5166N; -.
DR IntAct; P39981; 3.
DR STRING; 4932.YEL064C; -.
DR TCDB; 2.A.18.6.20; the amino acid/auxin permease (aaap) family.
DR PaxDb; P39981; -.
DR PRIDE; P39981; -.
DR EnsemblFungi; YEL064C_mRNA; YEL064C; YEL064C.
DR GeneID; 856645; -.
DR KEGG; sce:YEL064C; -.
DR SGD; S000000790; AVT2.
DR VEuPathDB; FungiDB:YEL064C; -.
DR eggNOG; KOG1305; Eukaryota.
DR GeneTree; ENSGT00940000157782; -.
DR HOGENOM; CLU_009020_4_1_1; -.
DR InParanoid; P39981; -.
DR OMA; CHHNTSF; -.
DR BioCyc; YEAST:G3O-30179-MON; -.
DR PRO; PR:P39981; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P39981; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..480
FT /note="Vacuolar amino acid transporter 2"
FT /id="PRO_0000093835"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 263..283
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 394..414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 447..467
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 21..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 480 AA; 53324 MW; 45799CBD3695B78E CRC64;
MSELGEYSKL ENKELRTEFE LTNFPFPGTT DNDSDDGSQG QNSLNIITPD MDDTLVNDVL
RENDKKSSMR MAFMNLANSI LGAGIITQPF AIKNAGILGG LLSYVALGFI VDWTLRLIVI
NLTLAGKRTY QGTVEHVMGK KGKLLILFTN GLFAFGGCIG YCIIIGDTIP HVLRAIFSQN
DGNVHFWLRR NVIIVMVTTF ISFPLSMKRN IEALSKASFL AVISMIIIVL TVVIRGPMLP
YDWKGHSLKL SDFFMKATIF RSLSVISFAL VCHHNTSFIF FSMRNRSVAK FTRLTHISII
ISVICCALMG YSGFAVFKEK TKGNVLNSFP GTDTAINIAR LCFGFNMLTT FPMEIFVLRD
VVGNLLHECN LIKNYDEHTQ LSGKQHVVIT SSLVFITMGI SLTTCNLGAL FELIGATTAS
TMAYILPPYT NLLLTSKKKS WKERLPFYLC ICFGFMIMII SSTQTIIDAV NGSDGQHCQI
