AVT3A_ARATH
ID AVT3A_ARATH Reviewed; 427 AA.
AC Q9FKY3; Q6NNP8;
DT 27-MAY-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Amino acid transporter AVT3A {ECO:0000305};
DE Short=AtAvt3A {ECO:0000303|PubMed:27925655};
DE AltName: Full=Aromatic and neutral amino acid transporter-like protein 3 {ECO:0000305};
GN Name=AVT3A {ECO:0000303|PubMed:27925655};
GN OrderedLocusNames=At5g65990 {ECO:0000312|Araport:AT5G65990};
GN ORFNames=K2A18.5 {ECO:0000312|EMBL:BAB10404.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-427.
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, NOMENCLATURE, SUBCELLULAR LOCATION, FUNCTION, MUTAGENESIS OF
RP GLU-241, AND TISSUE SPECIFICITY.
RX PubMed=27925655; DOI=10.1002/1873-3468.12507;
RA Fujiki Y., Teshima H., Kashiwao S., Kawano-Kawada M., Ohsumi Y.,
RA Kakinuma Y., Sekito T.;
RT "Functional identification of AtAVT3, a family of vacuolar amino acid
RT transporters, in Arabidopsis.";
RL FEBS Lett. 591:5-15(2017).
CC -!- FUNCTION: Translocates preferentially neutral amino acids and to a
CC lesser extent aromatic amino acids from the vacuole to the cytoplasm.
CC Requires ATP for function. {ECO:0000269|PubMed:27925655}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:27925655};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:27925655}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.8) subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAR92272.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB011474; BAB10404.1; -; Genomic_DNA.
DR EMBL; CP002688; AED98135.1; -; Genomic_DNA.
DR EMBL; BT026486; ABH04593.1; -; mRNA.
DR EMBL; BT011236; AAR92272.1; ALT_INIT; mRNA.
DR RefSeq; NP_201400.1; NM_125996.5.
DR AlphaFoldDB; Q9FKY3; -.
DR SMR; Q9FKY3; -.
DR IntAct; Q9FKY3; 2.
DR STRING; 3702.AT5G65990.1; -.
DR PaxDb; Q9FKY3; -.
DR PRIDE; Q9FKY3; -.
DR ProteomicsDB; 241018; -.
DR EnsemblPlants; AT5G65990.1; AT5G65990.1; AT5G65990.
DR GeneID; 836729; -.
DR Gramene; AT5G65990.1; AT5G65990.1; AT5G65990.
DR KEGG; ath:AT5G65990; -.
DR Araport; AT5G65990; -.
DR TAIR; locus:2156862; AT5G65990.
DR eggNOG; KOG1304; Eukaryota.
DR HOGENOM; CLU_009646_6_0_1; -.
DR InParanoid; Q9FKY3; -.
DR OMA; HAVWGRY; -.
DR OrthoDB; 464614at2759; -.
DR PhylomeDB; Q9FKY3; -.
DR PRO; PR:Q9FKY3; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FKY3; baseline and differential.
DR Genevisible; Q9FKY3; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005774; C:vacuolar membrane; IDA:UniProtKB.
DR GO; GO:0015173; F:aromatic amino acid transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015175; F:neutral amino acid transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0015801; P:aromatic amino acid transport; IDA:UniProtKB.
DR GO; GO:0015804; P:neutral amino acid transport; IDA:UniProtKB.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..427
FT /note="Amino acid transporter AVT3A"
FT /id="PRO_0000433108"
FT TOPO_DOM 1..42
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 43..63
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 64..69
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 70..90
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 91..122
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 123..143
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 144..157
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 158..178
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 179..186
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 187..207
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..227
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 228..248
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 249..262
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 263..283
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 284..300
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 301..321
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 322..339
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 340..360
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 361..362
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 363..383
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 384..396
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 397..417
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 418..427
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 241
FT /note="E->A: Abolishes transport activity."
FT /evidence="ECO:0000269|PubMed:27925655"
SQ SEQUENCE 427 AA; 46249 MW; AFB81143224C3D8A CRC64;
MRYDQEAGSS SHSLPSGSSS HSLPPTEDTP LLGPRTLSSQ PKTFANVFIA IVGAGVLGLP
YTFKKTGWLL GLLTLLFVSS LTFFCMMLLV HTRRKLESLS GFNSITSFGD LGESVCGPAG
RLVVDVMLVL SQSGFCVSYL IFVATTMANL LSRGTEHILG LDAASIYLWG CFPFQLGLNS
IPSLTHLAPL SIFADIVDVA ATLVVMVQDV FIFLKRRPPL RVFGGVSVFF YGLGVAVYAF
EGIGMVLPLE LEAKYKDKFG RALGLAMGLI SIMYGAFGLL GYMAYGEETK DIITTNLGTG
VVSTLVQLGL AINLFFTFPL MMQPVYEVVE RRLCSSRYSV WVRWATVLVV TLVALLVPNF
ADFLSLVGSS VCVVLGFVLP SLFHLQAFKN ELSITRIVVD VLVFLIGVMI AITGTWTAVH
EILTSKA
