AVT3B_ARATH
ID AVT3B_ARATH Reviewed; 413 AA.
AC F4ILY9; Q8GXJ5;
DT 27-MAY-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 60.
DE RecName: Full=Amino acid transporter AVT3B {ECO:0000305};
DE Short=AtAvt3B {ECO:0000303|PubMed:27925655};
DE AltName: Full=Aromatic and neutral amino acid transporter-like protein 1 {ECO:0000305};
GN Name=AVT3B {ECO:0000303|PubMed:27925655};
GN OrderedLocusNames=At2g42005 {ECO:0000312|Araport:AT2G42005};
GN ORFNames=T6D20 {ECO:0000312|EMBL:U90439};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP GENE FAMILY, NOMENCLATURE, SUBCELLULAR LOCATION, FUNCTION, AND TISSUE
RP SPECIFICITY.
RX PubMed=27925655; DOI=10.1002/1873-3468.12507;
RA Fujiki Y., Teshima H., Kashiwao S., Kawano-Kawada M., Ohsumi Y.,
RA Kakinuma Y., Sekito T.;
RT "Functional identification of AtAVT3, a family of vacuolar amino acid
RT transporters, in Arabidopsis.";
RL FEBS Lett. 591:5-15(2017).
CC -!- FUNCTION: Translocates preferentially neutral amino acids from the
CC vacuole to the cytoplasm. {ECO:0000269|PubMed:27925655}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:27925655};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:27925655}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.8) subfamily.
CC {ECO:0000305}.
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DR EMBL; U90439; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002685; AEC10062.1; -; Genomic_DNA.
DR EMBL; AK118204; BAC42826.1; -; mRNA.
DR RefSeq; NP_850361.1; NM_180030.3.
DR AlphaFoldDB; F4ILY9; -.
DR SMR; F4ILY9; -.
DR STRING; 3702.AT2G42005.1; -.
DR PaxDb; F4ILY9; -.
DR PRIDE; F4ILY9; -.
DR EnsemblPlants; AT2G42005.1; AT2G42005.1; AT2G42005.
DR GeneID; 818801; -.
DR Gramene; AT2G42005.1; AT2G42005.1; AT2G42005.
DR KEGG; ath:AT2G42005; -.
DR Araport; AT2G42005; -.
DR TAIR; locus:504955913; AT2G42005.
DR eggNOG; KOG1304; Eukaryota.
DR HOGENOM; CLU_009646_6_0_1; -.
DR InParanoid; F4ILY9; -.
DR OMA; ICTHWYQ; -.
DR OrthoDB; 464614at2759; -.
DR PRO; PR:F4ILY9; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4ILY9; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005774; C:vacuolar membrane; IDA:UniProtKB.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015175; F:neutral amino acid transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0015804; P:neutral amino acid transport; IDA:UniProtKB.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..413
FT /note="Amino acid transporter AVT3B"
FT /id="PRO_0000433106"
FT TOPO_DOM 1..27
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 28..48
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 49..54
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 55..75
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 76..103
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 104..124
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..145
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 146..166
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 167..174
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 175..195
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..207
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 208..228
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 229..249
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 250..270
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 271..288
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 289..309
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 310..331
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 332..352
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 353..355
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 356..376
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 377..390
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 391..411
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 412..413
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT CONFLICT 24
FT /note="S -> G (in Ref. 3; BAC42826)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 413 AA; 44962 MW; 2B7A1A60BAF1D1D3 CRC64;
MGLEEQGRAR EDTPLLGKGR PLSSKFKTFA NVFIAIVGAG VLGLPYAFKR TGWLMGLLTL
FSVAALINHC MMLLVHIRRK LGVSNIGSFG DLGFAACGNL GRFVVDILII LSQAGFCVGY
LIFIGNTLAN LSKPTKSTTL MSLRHLMGVS PKSLYIWGCF PFQLGLNSIK TLTHLAPLSI
FADVVDLGAM AVVIVEDIKI TVVQRPQVVA FGGMSVFFYG MGVAVYAFEG VGMVLPLESE
TKDKDKFGKV LALSMLFIAV MYGSFGVLGY MAFGDDTMDI ITANLGAGVV SSLVQLGLCI
NLFFTFPLMM NPVFEIVERR FWSGMYCVWL RWLLVLAVTL VALLVPNFAD FLSLVGSSVC
CALGFVLPSL FHLMVFKDEM EWKQRALDVG ILLLGVILGV SGTWSSLTEI FQE
