RRF_THET8
ID RRF_THET8 Reviewed; 185 AA.
AC Q9WX76; Q5SJZ4;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=Ribosome-recycling factor {ECO:0000255|HAMAP-Rule:MF_00040};
DE Short=RRF {ECO:0000255|HAMAP-Rule:MF_00040};
DE AltName: Full=Ribosome-releasing factor {ECO:0000255|HAMAP-Rule:MF_00040};
GN Name=frr {ECO:0000255|HAMAP-Rule:MF_00040}; OrderedLocusNames=TTHA0858;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10214965; DOI=10.1016/s0014-5793(99)00302-6;
RA Fujiwara T., Ito K., Nakayashiki T., Nakamura Y.;
RT "Amber mutations in ribosome recycling factors of Escherichia coli and
RT Thermus thermophilus: evidence for C-terminal modulator element.";
RL FEBS Lett. 447:297-302(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
RX PubMed=11073219; DOI=10.1017/s1355838200001060;
RA Toyoda T., Tin O.F., Ito K., Fujiwara T., Kumasaka T., Yamamoto M.,
RA Garber M.B., Nakamura Y.;
RT "Crystal structure combined with genetic analysis of the Thermus
RT thermophilus ribosome recycling factor shows that a flexible hinge may act
RT as a functional switch.";
RL RNA 6:1432-1444(2000).
CC -!- FUNCTION: Responsible for the release of ribosomes from messenger RNA
CC at the termination of protein biosynthesis. May increase the efficiency
CC of translation by recycling ribosomes from one round of translation to
CC another. {ECO:0000255|HAMAP-Rule:MF_00040}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00040}.
CC -!- SIMILARITY: Belongs to the RRF family. {ECO:0000255|HAMAP-
CC Rule:MF_00040}.
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DR EMBL; AB016498; BAA76865.1; -; Genomic_DNA.
DR EMBL; AP008226; BAD70681.1; -; Genomic_DNA.
DR RefSeq; WP_011172951.1; NC_006461.1.
DR RefSeq; YP_144124.1; NC_006461.1.
DR PDB; 1EH1; X-ray; 2.60 A; A=1-185.
DR PDB; 3J0D; EM; 11.10 A; J=1-185.
DR PDB; 3J0E; EM; 9.90 A; G=1-185.
DR PDB; 4V54; X-ray; 3.30 A; B6/D6=1-185.
DR PDB; 4V55; X-ray; 4.00 A; B6/D6=1-185.
DR PDB; 4V5A; X-ray; 3.50 A; AY/CY=1-185.
DR PDB; 4V5Y; X-ray; 4.45 A; B6/D6=1-185.
DR PDB; 6UCQ; X-ray; 3.50 A; 1w/2w=1-185.
DR PDBsum; 1EH1; -.
DR PDBsum; 3J0D; -.
DR PDBsum; 3J0E; -.
DR PDBsum; 4V54; -.
DR PDBsum; 4V55; -.
DR PDBsum; 4V5A; -.
DR PDBsum; 4V5Y; -.
DR PDBsum; 6UCQ; -.
DR AlphaFoldDB; Q9WX76; -.
DR BMRB; Q9WX76; -.
DR SMR; Q9WX76; -.
DR IntAct; Q9WX76; 3.
DR STRING; 300852.55772240; -.
DR EnsemblBacteria; BAD70681; BAD70681; BAD70681.
DR GeneID; 3170111; -.
DR KEGG; ttj:TTHA0858; -.
DR PATRIC; fig|300852.9.peg.852; -.
DR eggNOG; COG0233; Bacteria.
DR HOGENOM; CLU_073981_2_0_0; -.
DR OMA; FNPMNNG; -.
DR PhylomeDB; Q9WX76; -.
DR EvolutionaryTrace; Q9WX76; -.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0006415; P:translational termination; IEA:UniProtKB-UniRule.
DR CDD; cd00520; RRF; 1.
DR Gene3D; 1.10.132.20; -; 1.
DR HAMAP; MF_00040; RRF; 1.
DR InterPro; IPR002661; Ribosome_recyc_fac.
DR InterPro; IPR023584; Ribosome_recyc_fac_dom.
DR InterPro; IPR036191; RRF_sf.
DR PANTHER; PTHR20982; PTHR20982; 1.
DR Pfam; PF01765; RRF; 1.
DR SUPFAM; SSF55194; SSF55194; 1.
DR TIGRFAMs; TIGR00496; frr; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Protein biosynthesis; Reference proteome.
FT CHAIN 1..185
FT /note="Ribosome-recycling factor"
FT /id="PRO_0000167567"
FT HELIX 3..26
FT /evidence="ECO:0007829|PDB:1EH1"
FT HELIX 36..38
FT /evidence="ECO:0007829|PDB:1EH1"
FT STRAND 42..45
FT /evidence="ECO:0007829|PDB:1EH1"
FT STRAND 48..51
FT /evidence="ECO:0007829|PDB:1EH1"
FT HELIX 52..54
FT /evidence="ECO:0007829|PDB:1EH1"
FT STRAND 57..59
FT /evidence="ECO:0007829|PDB:1EH1"
FT STRAND 65..69
FT /evidence="ECO:0007829|PDB:1EH1"
FT HELIX 73..83
FT /evidence="ECO:0007829|PDB:1EH1"
FT STRAND 84..86
FT /evidence="ECO:0007829|PDB:1EH1"
FT STRAND 92..95
FT /evidence="ECO:0007829|PDB:1EH1"
FT STRAND 98..102
FT /evidence="ECO:0007829|PDB:1EH1"
FT HELIX 110..147
FT /evidence="ECO:0007829|PDB:1EH1"
FT HELIX 151..183
FT /evidence="ECO:0007829|PDB:1EH1"
SQ SEQUENCE 185 AA; 20994 MW; F3135ABF7F813328 CRC64;
MTLKELYAET RSHMQKSLEV LEHNLAGLRT GRANPALLLH LKVEYYGAHV PLNQIATVTA
PDPRTLVVQS WDQNALKAIE KAIRDSDLGL NPSNKGDALY INIPPLTEER RKDLVRAVRQ
YAEEGRVAIR NIRREALDKL KKLAKELHLS EDETKRAEAE IQKITDEFIA KADQLAEKKE
QEILG
