AVT5_SCHPO
ID AVT5_SCHPO Reviewed; 420 AA.
AC O74327;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Vacuolar amino acid transporter 5;
GN Name=avt5; Synonyms=avt8; ORFNames=SPBC1685.07c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION.
RX PubMed=19778961; DOI=10.1099/mic.0.034389-0;
RA Mukaiyama H., Kajiwara S., Hosomi A., Giga-Hama Y., Tanaka N., Nakamura T.,
RA Takegawa K.;
RT "Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial
RT sporulation during nitrogen starvation.";
RL Microbiology 155:3816-3826(2009).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=20388511; DOI=10.1016/j.febslet.2010.04.012;
RA Chardwiriyapreecha S., Mukaiyama H., Sekito T., Iwaki T., Takegawa K.,
RA Kakinuma Y.;
RT "Avt5p is required for vacuolar uptake of amino acids in the fission yeast
RT Schizosaccharomyces pombe.";
RL FEBS Lett. 584:2339-2345(2010).
CC -!- FUNCTION: Vacuolar amino acid transporter involved in the vacuolar
CC uptake of histidine, glutamate, tyrosine, arginine, lysine, and serine.
CC Required for sporulation. {ECO:0000269|PubMed:19778961,
CC ECO:0000269|PubMed:20388511}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:20388511};
CC Multi-pass membrane protein {ECO:0000269|PubMed:20388511}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC {ECO:0000305}.
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DR EMBL; CU329671; CAA20055.1; -; Genomic_DNA.
DR PIR; T39523; T39523.
DR RefSeq; NP_595211.1; NM_001021118.2.
DR AlphaFoldDB; O74327; -.
DR SMR; O74327; -.
DR BioGRID; 276619; 2.
DR STRING; 4896.SPBC1685.07c.1; -.
DR iPTMnet; O74327; -.
DR MaxQB; O74327; -.
DR PaxDb; O74327; -.
DR EnsemblFungi; SPBC1685.07c.1; SPBC1685.07c.1:pep; SPBC1685.07c.
DR GeneID; 2540081; -.
DR KEGG; spo:SPBC1685.07c; -.
DR PomBase; SPBC1685.07c; avt5.
DR VEuPathDB; FungiDB:SPBC1685.07c; -.
DR eggNOG; KOG1305; Eukaryota.
DR HOGENOM; CLU_009020_1_1_1; -.
DR InParanoid; O74327; -.
DR OMA; QFWITAF; -.
DR PhylomeDB; O74327; -.
DR PRO; PR:O74327; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IDA:PomBase.
DR GO; GO:0071627; C:integral component of fungal-type vacuolar membrane; IC:PomBase.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0061459; F:L-arginine transmembrane transporter activity; IMP:PomBase.
DR GO; GO:0005313; F:L-glutamate transmembrane transporter activity; IMP:PomBase.
DR GO; GO:0005290; F:L-histidine transmembrane transporter activity; IMP:PomBase.
DR GO; GO:0015189; F:L-lysine transmembrane transporter activity; IMP:PomBase.
DR GO; GO:0015194; F:L-serine transmembrane transporter activity; IMP:PomBase.
DR GO; GO:0005302; F:L-tyrosine transmembrane transporter activity; IMP:PomBase.
DR GO; GO:0032975; P:amino acid transmembrane import into vacuole; IMP:PomBase.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0090518; P:L-arginine transmembrane import into vacuole; IMP:PomBase.
DR GO; GO:1901481; P:L-glutamate import involved in cellular response to nitrogen starvation; IMP:PomBase.
DR GO; GO:0090515; P:L-glutamate transmembrane import into vacuole; IMP:PomBase.
DR GO; GO:0090513; P:L-histidine transmembrane import into vacuole; IMP:PomBase.
DR GO; GO:1901482; P:L-lysine import into vacuole involved in cellular response to nitrogen starvation; IMP:PomBase.
DR GO; GO:0090517; P:L-lysine transmembrane import into vacuole; IMP:PomBase.
DR GO; GO:0090516; P:L-serine transmembrane import into vacuole; IMP:PomBase.
DR GO; GO:0090514; P:L-tyrosine transmembrane import into vacuole; IMP:PomBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 3: Inferred from homology;
KW Amino-acid transport; Membrane; Reference proteome; Sporulation;
KW Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..420
FT /note="Vacuolar amino acid transporter 5"
FT /id="PRO_0000316193"
FT TOPO_DOM 1..19
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 41..49
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 71..96
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 118..141
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 163..166
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..195
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 217..240
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..278
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 300..326
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 348..349
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 371..394
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 416..420
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
SQ SEQUENCE 420 AA; 45462 MW; D76B8834CE66E170 CRC64;
MSGYSPLSSG PADVHIGKAG FFSSVINLAN TILGAGILSL PNAFTKTGLL FGCLTIVFSA
FASFLGLYFV SQCAARLPRG KASFAAVAKH TFPSLAVVFD ASIAVKCFGV AVSYLVIVGD
LMPQIAPSLG LSSPMFLRRQ TWIVFALFVL TPLSFLKRLD SLRHTSVISL IALCYLVFIV
LYHFIIGDTV KGEIRYFVPE SGFGYLSVLP VFVFGFTCHQ NAFSVINEVR NFSQGFVNFT
MFTAIISSTL LYLLVAITGY LSFGSLASGN IIAMYDNTSI WIIGGKLAIV VLVLFSYPLQ
CHPCRNSVYQ AIRRSYSAHD MSDGYHAVIT LCILLFTHSL ALLLSSLEMV LAFVGSTGST
FISFILPGSL YYFFSHKVAS PGNSSPLQLR ISRAFAAGLA IYGTVVMILC LNINIAKLSH
