AVT5_YEAST
ID AVT5_YEAST Reviewed; 459 AA.
AC P38176; D6VPR5;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Vacuolar amino acid transporter 5;
GN Name=AVT5; OrderedLocusNames=YBL089W; ORFNames=YBL0703;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7502586; DOI=10.1002/yea.320111112;
RA Obermaier B., Gassenhuber J., Piravandi E., Domdey H.;
RT "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces
RT cerevisiae chromosome II.";
RL Yeast 11:1103-1112(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11274162; DOI=10.1074/jbc.m008028200;
RA Russnak R., Konczal D., McIntire S.L.;
RT "A family of yeast proteins mediating bidirectional vacuolar amino acid
RT transport.";
RL J. Biol. Chem. 276:23849-23857(2001).
RN [5]
RP IDENTIFICATION OF PROBABLE INITIATION SITE.
RX PubMed=12748633; DOI=10.1038/nature01644;
RA Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT "Sequencing and comparison of yeast species to identify genes and
RT regulatory elements.";
RL Nature 423:241-254(2003).
CC -!- FUNCTION: Probable amino acid transporter of unknown specificity.
CC {ECO:0000269|PubMed:11274162}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:11274162};
CC Multi-pass membrane protein {ECO:0000269|PubMed:11274162}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC {ECO:0000305}.
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DR EMBL; X79489; CAA56013.1; -; Genomic_DNA.
DR EMBL; Z35850; CAA84910.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07035.1; -; Genomic_DNA.
DR PIR; S45413; S45413.
DR RefSeq; NP_009464.2; NM_001178329.1.
DR AlphaFoldDB; P38176; -.
DR BioGRID; 32615; 191.
DR DIP; DIP-2846N; -.
DR IntAct; P38176; 4.
DR MINT; P38176; -.
DR STRING; 4932.YBL089W; -.
DR TCDB; 2.A.18.6.10; the amino acid/auxin permease (aaap) family.
DR PaxDb; P38176; -.
DR EnsemblFungi; YBL089W_mRNA; YBL089W; YBL089W.
DR GeneID; 852189; -.
DR KEGG; sce:YBL089W; -.
DR SGD; S000000185; AVT5.
DR VEuPathDB; FungiDB:YBL089W; -.
DR eggNOG; KOG1305; Eukaryota.
DR GeneTree; ENSGT00940000170090; -.
DR HOGENOM; CLU_009020_1_1_1; -.
DR InParanoid; P38176; -.
DR OMA; KARMQNV; -.
DR BioCyc; YEAST:G3O-28978-MON; -.
DR PRO; PR:P38176; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38176; protein.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; ISS:SGD.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0061459; F:L-arginine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005313; F:L-glutamate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005290; F:L-histidine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015189; F:L-lysine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015194; F:L-serine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005302; F:L-tyrosine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; ISS:SGD.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0055085; P:transmembrane transport; ISS:SGD.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 3: Inferred from homology;
KW Amino-acid transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..459
FT /note="Vacuolar amino acid transporter 5"
FT /id="PRO_0000093838"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..226
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 278..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 364..384
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 386..406
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 434..454
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 335..354
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 335..353
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 459 AA; 50892 MW; 8D3D533A63CD190D CRC64;
MPSNVRSGVL TLLHTACGAG VLAMPFAFKP FGLMPGLITL TFCGICSLCG LLLQTRIAKY
VPKSENASFA KLTQLINPSI SVVFDFAIAV KCFGVGVSYL IIVGDLVPQI VQSIFYRNDD
NMSGSQEHHM FLDRRLYITL IIVFVISPLC FKRSLNSLRY ASMIAIVSVA YLSGLIIYHF
VNRHQLERGQ VYFMVPHGDS QSHSPLTTLP IFVFAYTCHH NMFSVINEQV DKSFKVIRRI
PIFAIVLAYF LYIIIGGTGY MTFGENIVGN ILTLYPNSIS TTIGRLAMLL LVMLAFPLQC
HPCRSSVKNI IIFIENFRKG KLYDNRASFI PLDNFNSEDP QEAPTQQNNE EPNLRSESLR
HINIITLCIL LFSYLLAISI TSLAKVLAIV GATGSTSISF ILPGLFGYKL IGSEFTGTNE
RVPTSIKIFK YLSLSLFIWG IAVMVASLSA IVFLGTSSH
