RRG1_YEAST
ID RRG1_YEAST Reviewed; 365 AA.
AC Q12167; D6VS51; Q6Q5U4;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Required for respiratory growth protein 1, mitochondrial;
GN Name=RRG1; OrderedLocusNames=YDR065W; ORFNames=D4258;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8789263;
RX DOI=10.1002/(sici)1097-0061(199601)12:1<85::aid-yea890>3.0.co;2-u;
RA Brandt P., Ramlow S., Otto B., Bloecker H.;
RT "Nucleotide sequence analysis of a 32,500 bp region of the right arm of
RT Saccharomyces cerevisiae chromosome IV.";
RL Yeast 12:85-90(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP FUNCTION.
RX PubMed=16168084; DOI=10.1186/gb-2005-6-9-r77;
RA Eide D.J., Clark S., Nair T.M., Gehl M., Gribskov M., Guerinot M.L.,
RA Harper J.F.;
RT "Characterization of the yeast ionome: a genome-wide analysis of nutrient
RT mineral and trace element homeostasis in Saccharomyces cerevisiae.";
RL Genome Biol. 6:R77.1-R77.13(2005).
RN [8]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=16823961; DOI=10.1021/pr050477f;
RA Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.;
RT "Toward the complete yeast mitochondrial proteome: multidimensional
RT separation techniques for mitochondrial proteomics.";
RL J. Proteome Res. 5:1543-1554(2006).
RN [9]
RP FUNCTION.
RX PubMed=19751518; DOI=10.1186/gb-2009-10-9-r95;
RA Merz S., Westermann B.;
RT "Genome-wide deletion mutant analysis reveals genes required for
RT respiratory growth, mitochondrial genome maintenance and mitochondrial
RT protein synthesis in Saccharomyces cerevisiae.";
RL Genome Biol. 10:R95.1-R95.18(2009).
RN [10]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS], AND SUBCELLULAR LOCATION.
RX PubMed=19756047; DOI=10.1038/msb.2009.64;
RA Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.;
RT "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals
RT new roles for protein glycosylation in eukaryotes.";
RL Mol. Syst. Biol. 5:308-308(2009).
CC -!- FUNCTION: Essential for respiratory growth and required for
CC mitochondrial protein synthesis. Required for vacuolar acidification.
CC {ECO:0000269|PubMed:16168084, ECO:0000269|PubMed:19751518}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:16823961, ECO:0000269|PubMed:19756047}.
CC -!- PTM: N-glycosylated. Glycosylation is important for correct
CC localization of the protein. {ECO:0000269|PubMed:19756047}.
CC -!- MISCELLANEOUS: Present with 1670 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the RRG1 family. {ECO:0000305}.
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DR EMBL; X84162; CAA58981.1; -; Genomic_DNA.
DR EMBL; Z49209; CAA89094.1; -; Genomic_DNA.
DR EMBL; Z74361; CAA98883.1; -; Genomic_DNA.
DR EMBL; AY557697; AAS56023.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11911.1; -; Genomic_DNA.
DR PIR; S54049; S54049.
DR RefSeq; NP_010350.3; NM_001180373.3.
DR AlphaFoldDB; Q12167; -.
DR SMR; Q12167; -.
DR BioGRID; 32120; 71.
DR IntAct; Q12167; 1.
DR MINT; Q12167; -.
DR STRING; 4932.YDR065W; -.
DR PaxDb; Q12167; -.
DR PRIDE; Q12167; -.
DR EnsemblFungi; YDR065W_mRNA; YDR065W; YDR065W.
DR GeneID; 851637; -.
DR KEGG; sce:YDR065W; -.
DR SGD; S000002472; RRG1.
DR VEuPathDB; FungiDB:YDR065W; -.
DR eggNOG; ENOG502RYGE; Eukaryota.
DR HOGENOM; CLU_062256_0_0_1; -.
DR InParanoid; Q12167; -.
DR OMA; WYRYTLR; -.
DR BioCyc; YEAST:G3O-29672-MON; -.
DR PRO; PR:Q12167; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q12167; protein.
DR GO; GO:0099617; C:matrix side of mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0000002; P:mitochondrial genome maintenance; IMP:SGD.
DR GO; GO:0097745; P:mitochondrial tRNA 5'-end processing; IMP:SGD.
DR GO; GO:0007035; P:vacuolar acidification; IMP:SGD.
PE 1: Evidence at protein level;
KW Glycoprotein; Mitochondrion; Reference proteome.
FT CHAIN 1..365
FT /note="Required for respiratory growth protein 1,
FT mitochondrial"
FT /id="PRO_0000244438"
FT CONFLICT 364
FT /note="G -> D (in Ref. 2; AAS56023)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 365 AA; 42829 MW; 3DCCF51829BFDBB2 CRC64;
MAQNFGKIPS HKSYVLSLYR TVLRNIPKCC HSYAFQYEIK KTLSIQLFKH KHDKSSWSVY
TLLNEFSLLN NCLLEGKLQE IKNLMKPLKK MKKQLKTTKI LNSLTSLGDV KTNDPEEVRR
FHVLSAYIKR KQDLGLLPAY IPKTYQHKLL LPLALNEHAC LKLFHIQQKL KNGPPSAGLS
YTKEGRNQIW FVRSPINKGR QQSKKLGILI RKERKDSQKN IDNLNFCEIN AAWALHEAIW
EEYLESKKII KVNLPKYLEY AANIPKSTKC NPSSQYQKVK EWVDPVREIM FELHSKSFQR
VEYFNKYKEK LLKNGGQLAY FDKKSKEMYA KRLTLFRKMS KETLPYVTLF IEGRDLPSVL
AKYGF
