AVT7_YEAST
ID AVT7_YEAST Reviewed; 490 AA.
AC P40501; D6VVJ9;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Vacuolar amino acid transporter 7;
GN Name=AVT7; OrderedLocusNames=YIL088C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11274162; DOI=10.1074/jbc.m008028200;
RA Russnak R., Konczal D., McIntire S.L.;
RT "A family of yeast proteins mediating bidirectional vacuolar amino acid
RT transport.";
RL J. Biol. Chem. 276:23849-23857(2001).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Probable amino acid transporter of unknown specificity.
CC {ECO:0000269|PubMed:11274162}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:11274162};
CC Multi-pass membrane protein {ECO:0000269|PubMed:11274162}.
CC -!- MISCELLANEOUS: Present with 195 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC {ECO:0000305}.
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DR EMBL; Z46728; CAA86706.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08465.1; -; Genomic_DNA.
DR PIR; S49792; S49792.
DR RefSeq; NP_012178.1; NM_001179436.1.
DR AlphaFoldDB; P40501; -.
DR BioGRID; 34905; 57.
DR DIP; DIP-7299N; -.
DR STRING; 4932.YIL088C; -.
DR TCDB; 2.A.18.6.19; the amino acid/auxin permease (aaap) family.
DR MaxQB; P40501; -.
DR PaxDb; P40501; -.
DR PRIDE; P40501; -.
DR EnsemblFungi; YIL088C_mRNA; YIL088C; YIL088C.
DR GeneID; 854721; -.
DR KEGG; sce:YIL088C; -.
DR SGD; S000001350; AVT7.
DR VEuPathDB; FungiDB:YIL088C; -.
DR eggNOG; KOG1305; Eukaryota.
DR HOGENOM; CLU_009020_1_1_1; -.
DR InParanoid; P40501; -.
DR OMA; FAFTGHQ; -.
DR BioCyc; YEAST:G3O-31348-MON; -.
DR PRO; PR:P40501; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P40501; protein.
DR GO; GO:0000324; C:fungal-type vacuole; IDA:SGD.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0005774; C:vacuolar membrane; IDA:SGD.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; ISA:SGD.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0061459; F:L-arginine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005313; F:L-glutamate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005290; F:L-histidine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015189; F:L-lysine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015194; F:L-serine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005302; F:L-tyrosine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0006865; P:amino acid transport; IMP:SGD.
DR GO; GO:0043937; P:regulation of sporulation; IMP:SGD.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..490
FT /note="Vacuolar amino acid transporter 7"
FT /id="PRO_0000093840"
FT TOPO_DOM 1..6
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..34
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 56..84
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..108
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 130..143
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 165..190
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212..221
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 243..264
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 286..397
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..418
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 419..428
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 429..449
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 450..463
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 464..484
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 485..490
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT REGION 355..374
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 490 AA; 53737 MW; D3D15CD8253220A1 CRC64;
MEATSSALSS TANLVKTIVG AGTLAIPYSF KSDGVLVGVI LTLLAAVTSG LGLFVLSKCS
KTLINPRNSS FFTLCMLTYP TLAPIFDLAM IVQCFGVGLS YLVLIGDLFP GLFGGERNYW
IIASAVIIIP LCLVKKLDQL KYSSILGLFA LAYISILVFS HFVFELGKGE LTNILRNDIC
WWKIHDFKGL LSTFSIIIFA FTGSMNLFPM INELKDNSME NITFVINNSI SLSTALFLIV
GLSGYLTFGN ETLGNLMLNY DPNSIWIVIG KFCLGSMLIL SFPLLFHPLR IAVNNVIIWI
EITYGGANPE EDPQVSEYTR ASNLRPISMT VEDPAQPSDA LDATSYNEQE CLLPNGNFDN
GSIESQENNN DERGTMAVAG DNEHHAPFVK SRFYWITALL LISMYTLALS VQSFALVLSF
VGATGSTSIS FTLPGLLGYK LIGLDSLAIG KMIPPKDRFY KRCSLLLVFY GLSVMFLSLY
VTVFNRSDEA
