RRN5_YEAST
ID RRN5_YEAST Reviewed; 363 AA.
AC Q02983; D6VYD5; Q12061;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=RNA polymerase I-specific transcription initiation factor RRN5;
GN Name=RRN5; OrderedLocusNames=YLR141W; ORFNames=L3165, L9606.3;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND IDENTIFICATION IN THE UAF COMPLEX.
RC STRAIN=NOY697;
RX PubMed=8846924; DOI=10.1101/gad.10.7.887;
RA Keys D.A., Lee B.-S., Dodd J.A., Nguyen T.T., Vu L., Fantino E.,
RA Burson L.M., Nogi Y., Nomura M.;
RT "Multiprotein transcription factor UAF interacts with the upstream element
RT of the yeast RNA polymerase I promoter and forms a stable preinitiation
RT complex.";
RL Genes Dev. 10:887-903(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION OF THE UAF COMPLEX.
RX PubMed=9837969; DOI=10.1074/jbc.273.50.33795;
RA Keener J., Josaitis C.A., Dodd J.A., Nomura M.;
RT "Reconstitution of yeast RNA polymerase I transcription in vitro from
RT purified components. TATA-binding protein is not required for basal
RT transcription.";
RL J. Biol. Chem. 273:33795-33802(1998).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
CC -!- FUNCTION: Component of the UAF (upstream activation factor) complex
CC which interacts with the upstream element of the RNA polymerase I
CC promoter and forms a stable preinitiation complex. Together with
CC SPT15/TBP UAF seems to stimulate basal transcription to a fully
CC activated level. {ECO:0000269|PubMed:9837969}.
CC -!- SUBUNIT: Component of the UAF (upstream activation factor) complex
CC which consists of UAF30, RRN5, RRN9, RRN10, and histones H3 and H4.
CC {ECO:0000269|PubMed:8846924}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:14562095}.
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DR EMBL; U43680; AAC49257.1; -; Genomic_DNA.
DR EMBL; U53881; AAB82391.1; -; Genomic_DNA.
DR EMBL; X91258; CAA62660.1; -; Genomic_DNA.
DR EMBL; Z73312; CAA97713.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09451.1; -; Genomic_DNA.
DR PIR; S59337; S59337.
DR RefSeq; NP_013242.1; NM_001182028.1.
DR AlphaFoldDB; Q02983; -.
DR SMR; Q02983; -.
DR BioGRID; 31409; 95.
DR ComplexPortal; CPX-1101; RNA polymerase I upstream activating factor complex.
DR DIP; DIP-3964N; -.
DR IntAct; Q02983; 5.
DR STRING; 4932.YLR141W; -.
DR MaxQB; Q02983; -.
DR PaxDb; Q02983; -.
DR PRIDE; Q02983; -.
DR EnsemblFungi; YLR141W_mRNA; YLR141W; YLR141W.
DR GeneID; 850832; -.
DR KEGG; sce:YLR141W; -.
DR SGD; S000004131; RRN5.
DR VEuPathDB; FungiDB:YLR141W; -.
DR eggNOG; ENOG502RY38; Eukaryota.
DR HOGENOM; CLU_024706_0_0_1; -.
DR InParanoid; Q02983; -.
DR OMA; DEEGHCE; -.
DR BioCyc; YEAST:G3O-32280-MON; -.
DR PRO; PR:Q02983; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q02983; protein.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0000500; C:RNA polymerase I upstream activating factor complex; IDA:SGD.
DR GO; GO:0000182; F:rDNA binding; IBA:GO_Central.
DR GO; GO:0001181; F:RNA polymerase I general transcription initiation factor activity; IDA:SGD.
DR GO; GO:0006325; P:chromatin organization; IMP:SGD.
DR GO; GO:0042790; P:nucleolar large rRNA transcription by RNA polymerase I; IDA:SGD.
DR GO; GO:0045943; P:positive regulation of transcription by RNA polymerase I; IDA:ComplexPortal.
DR GO; GO:0006361; P:transcription initiation from RNA polymerase I promoter; IBA:GO_Central.
DR InterPro; IPR039601; Rrn5.
DR PANTHER; PTHR28079; PTHR28079; 2.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..363
FT /note="RNA polymerase I-specific transcription initiation
FT factor RRN5"
FT /id="PRO_0000097447"
FT REGION 301..344
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 25
FT /note="A -> R (in Ref. 1; AAC49257)"
FT /evidence="ECO:0000305"
FT CONFLICT 197..198
FT /note="SA -> RP (in Ref. 1; AAC49257)"
FT /evidence="ECO:0000305"
FT CONFLICT 260
FT /note="R -> G (in Ref. 1; AAC49257)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 363 AA; 41721 MW; E3C25C1B1E336820 CRC64;
MEHQQLRKYV ELYNKEVEEF YNGAASGRPA EFHPSKVHVK SIHEKAGTAN AGVEISSVGV
DWDSEEKNTF FWCLSRYSIH RVDEWRSLLP RKSAMEILGY YRLLRRASAS ARSRKAGDDG
APIAYEMSAE WVALETKLSE TVMAITEGAA EVADEEGHCE GLIDYESWKR RWVAIYSHSR
IAEIRPLPRH ALPLSRSATQ TLERCVSRYT RTLLWCTALA GMASRSVSAR AAESRGHKSL
PTVVTRRQVE RALCTEARSR DLHVLPRRIV LTLRKWELDY PREGKLFRTK EMAHLFLQSQ
LSRRDAPPVH QDENQENQEN QENQEQDNTA SEGESEAERD EIDEADLFRS ALHENQLLKW
LSK
