AWA1_YEASK
ID AWA1_YEASK Reviewed; 1713 AA.
AC Q8TGE1; G2WM83; Q76C74;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Cell wall protein AWA1;
DE Flags: Precursor;
GN Name=AWA1; ORFNames=SYK7_061821;
OS Saccharomyces cerevisiae (strain Kyokai no. 7 / NBRC 101557) (Baker's
OS yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=721032;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, AND BIOTECHNOLOGY.
RC STRAIN=Kyokai no. 7 / NBRC 101557;
RX PubMed=11916725; DOI=10.1128/aem.68.4.2018-2025.2002;
RA Shimoi H., Sakamoto K., Okuda M., Atthi R., Iwashita K., Ito K.;
RT "The Awa1 gene is required for the foam-forming phenotype and cell surface
RT hydrophobicity of sake yeast.";
RL Appl. Environ. Microbiol. 68:2018-2025(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Kyokai no. 7 / NBRC 101557, and Kyokai no. 701;
RX PubMed=16233582; DOI=10.1263/jbb.97.14;
RA Miyashita K., Sakamoto K., Kitagaki H., Iwashita K., Ito K., Shimoi H.;
RT "Cloning and analysis of the AWA1 gene of a nonfoaming mutant of a sake
RT Yeast.";
RL J. Biosci. Bioeng. 97:14-18(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Kyokai no. 7 / NBRC 101557;
RX PubMed=21900213; DOI=10.1093/dnares/dsr029;
RA Akao T., Yashiro I., Hosoyama A., Kitagaki H., Horikawa H., Watanabe D.,
RA Akada R., Ando Y., Harashima S., Inoue T., Inoue Y., Kajiwara S.,
RA Kitamoto K., Kitamoto N., Kobayashi O., Kuhara S., Masubuchi T.,
RA Mizoguchi H., Nakao Y., Nakazato A., Namise M., Oba T., Ogata T., Ohta A.,
RA Sato M., Shibasaki S., Takatsume Y., Tanimoto S., Tsuboi H., Nishimura A.,
RA Yoda K., Ishikawa T., Iwashita K., Fujita N., Shimoi H.;
RT "Whole-genome sequencing of sake yeast Saccharomyces cerevisiae Kyokai no.
RT 7.";
RL DNA Res. 18:423-434(2011).
CC -!- FUNCTION: Involved in cell wall organization and biosynthesis. Confers
CC cell surface hydrophobicity (CSH).
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000269|PubMed:11916725}. Membrane {ECO:0000269|PubMed:11916725};
CC Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:11916725}.
CC Note=Covalently-linked GPI-modified cell wall protein (GPI-CWP).
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
CC -!- POLYMORPHISM: Half of the downstream region (residues 1035 to 1713) of
CC the AWA1 gene was lost in the nonfoaming strain K701 due to a
CC chromosomal recombination event.
CC -!- BIOTECHNOLOGY: Responsible for the thick foam layer formation on the
CC sake mash during the fermentation process.
CC {ECO:0000269|PubMed:11916725}.
CC -!- MISCELLANEOUS: 'Awa' means 'foam' in Japanese.
CC -!- SIMILARITY: Belongs to the SRP1/TIP1 family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=Of froth and haze - Issue 78
CC of January 2007;
CC URL="https://web.expasy.org/spotlight/back_issues/078";
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DR EMBL; AB071164; BAB85832.1; -; Genomic_DNA.
DR EMBL; AB110100; BAD06576.1; -; Genomic_DNA.
DR EMBL; AB110101; BAD06577.1; -; Genomic_DNA.
DR EMBL; DG000051; GAA26176.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8TGE1; -.
DR SMR; Q8TGE1; -.
DR EnsemblFungi; GAA26176; GAA26176; SYK7_061821.
DR SGD; S000029703; AWA1.
DR HOGENOM; CLU_240507_0_0_1; -.
DR Proteomes; UP000001608; Chromosome XV.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR InterPro; IPR000992; SRP1_TIP1.
DR Pfam; PF00660; SRP1_TIP1; 1.
DR PROSITE; PS00724; SRP1_TIP1; 1.
PE 1: Evidence at protein level;
KW Cell wall; Cell wall biogenesis/degradation; Glycoprotein; GPI-anchor;
KW Lipoprotein; Membrane; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..1692
FT /note="Cell wall protein AWA1"
FT /id="PRO_0000268175"
FT PROPEP 1693..1713
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000268176"
FT REGION 80..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 256..327
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 359..939
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1582..1603
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 1692
FT /note="GPI-anchor amidated alanine"
FT /evidence="ECO:0000255"
FT CARBOHYD 34
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1133
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1241
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 1278
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 1036..1713
FT /note="Missing (in strain: Kyokai no. 701, non-foaming
FT mutant derivative of Kyokai no. 7)"
SQ SEQUENCE 1713 AA; 166884 MW; 9321A296EB586404 CRC64;
MFNRFNKLQA ALALVLYSQS ALGQYYTSSS IASNSSTAVS STSSGSVSIS SSIELTSSTS
DVSSSLTELT SSFTEVSSSI APSTSSSEVS SSITSSGSSV SGSSSITSSG SSVSSSSPYD
ERFNSLDLSV HVSAGFSAGV SVGLEPSATT ASVTTTLSPY DERVNLIELG VYVSDMRAHL
VEYLLFQAAH STEPHPTEIA AAFLDHGDFT TRLTGISGDE VTRMITGVPW YSTRLKPAIS
EALAKDGIYT AIPTSTSTTS DTYISSSSPS QVTSSAEPTT VSGVTSSVEP TRSSQVTSSA
EPTTVSEITS SAEPLSSSKA TTSAESISSN QITISSELIV SSVITSSSEI PSSIEVLTSS
GISSSVEPTS LVGPSSDESI SSTESLSATS TPLAVSSTVV TSSTDSVSPN IPFSEISSSP
ESSTAITSGS SSATESGSSV SGSTSATESG SSASGSSSAT ESGSSVSGST SATESGSASS
VPSSSGSVTE SGSSSSASES SITQSGTASG SSVSSTSGSV TQSGSSVSGS SASSAPGISS
SIPQSTSSAS TASGSITSGT LTSITSGSSS ATESGSSVSG SSSATESGSS VSGSTSATES
GSSVSGSTSA TESGSSASGS SSATESGSSV SGSTSATESG SSVSGSTSAT ESGSSASGSS
SATESGSASS VPSSSGSVTE SGSSSSASES SITQSGTASG SSASSTSGSV TQSGSSVSGS
SASSAPGISS SIPQSTSSAS TASGSITSGT LTSITSGSSS ATESGSSASG SSSATESGSS
VSGSTSATES GSSVSGSTSA TESGSSASGS SSATESGSSV SGSTSATESG SSASGSSSAT
ESGSASSVPS SSGSVTESGS SSSASESSIT QSGTASGSSA SSTSGSVTQS GSSVSGSSAS
STSGSVTQSG SSVSGSSASS APGISSSIPQ STSSASTASG SITSGTLTSI TSSASSASAT
ASNSLSSSDG TIYLPTTTIS GDLTLTGKVI ATEGVVVAAG AKLTLLDGDK YSFSADLKVY
GDLLVKKSKE TYPGTEFDIS GENFDVTGNF NAEESAATSA SIYSFTPSSF DNSGDISLSL
SKSKKGEVTF SPYSNSGAFS FSNAILNGGS VSGLQRRDDT EGSVNNGEIN LDNGSTYVIV
EPVSGKGTVN IISGNLYLHY PDTFTGQTVV FKGEGVLAVD PTESNTTPIP VVGYTGENQI
AITADVTALS YDSATGVLTA TQGNSQFSFS IGTGFSSSGF NVSEGTFAGA YAYYLNYGGV
VASSATPSST STTSGATNST SGSTSFGASV TGSTASTSFG ASVTGSTAST LISGSPSVYT
TTLTYATTTS TVVVSCSETT DSNGNVYTIT TTVPCSSTTA TITSCDETGC HVTTSTGTVA
TETVSSKSYT TVTVTHCDNN GCNTKTVTSE APEATTTTVS PKTYTTATVT QCDDNGCSTK
TVTSECPEET SATTTSPKSY TTVTVTHCDD NGCNTKTVTS EAPEATTTTV SPKTYTTATV
TQCDDNGCST KTVTSECPEE TSATTTSPKS YTTVTVTHCD DNGCNTKTVT SEAPEATTTT
VSPKTYTTAT VTQCDDNGCS TKTVTSEAPK ETSETSETSA APKTYTTATV TQCDDNGCNV
KIITSQIPEA TSTVTATSAS PKSYTTVTSE GSKATSLTTA ISKASSAIST YSKSAAPIKT
STGIIVQSEG IAAGLNANTL NALVGIFVLA FFN
