RRN9_YEAST
ID RRN9_YEAST Reviewed; 365 AA.
AC P53437; D6W096; E9P918;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=RNA polymerase I-specific transcription initiation factor RRN9;
GN Name=RRN9; OrderedLocusNames=YMR270C; ORFNames=YM8156.12C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND IDENTIFICATION IN THE UAF COMPLEX.
RC STRAIN=NOY698;
RX PubMed=8846924; DOI=10.1101/gad.10.7.887;
RA Keys D.A., Lee B.-S., Dodd J.A., Nguyen T.T., Vu L., Fantino E.,
RA Burson L.M., Nogi Y., Nomura M.;
RT "Multiprotein transcription factor UAF interacts with the upstream element
RT of the yeast RNA polymerase I promoter and forms a stable preinitiation
RT complex.";
RL Genes Dev. 10:887-903(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP INTERACTION WITH SPT15 AND RRN7.
RX PubMed=8895657; DOI=10.1101/gad.10.20.2551;
RA Steffan J.S., Keys D.A., Dodd J.A., Nomura M.;
RT "The role of TBP in rDNA transcription by RNA polymerase I in Saccharomyces
RT cerevisiae: TBP is required for upstream activation factor-dependent
RT recruitment of core factor.";
RL Genes Dev. 10:2551-2563(1996).
RN [6]
RP FUNCTION OF THE UAF COMPLEX.
RX PubMed=9837969; DOI=10.1074/jbc.273.50.33795;
RA Keener J., Josaitis C.A., Dodd J.A., Nomura M.;
RT "Reconstitution of yeast RNA polymerase I transcription in vitro from
RT purified components. TATA-binding protein is not required for basal
RT transcription.";
RL J. Biol. Chem. 273:33795-33802(1998).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
CC -!- FUNCTION: Component of the UAF (upstream activation factor) complex
CC which interacts with the upstream element of the RNA polymerase I
CC promoter and forms a stable preinitiation complex. Together with
CC SPT15/TBP UAF seems to stimulate basal transcription to a fully
CC activated level. {ECO:0000269|PubMed:9837969}.
CC -!- SUBUNIT: Component of the UAF (upstream activation factor) complex
CC which consists of UAF30, RRN5, RRN9, RRN10, and histones H3 and H4.
CC Interacts with SPT15 and RRN7. {ECO:0000269|PubMed:8846924,
CC ECO:0000269|PubMed:8895657}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:14562095}.
CC -!- SIMILARITY: Belongs to the RRN9 family. {ECO:0000305}.
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DR EMBL; U43681; AAC49258.1; -; Genomic_DNA.
DR EMBL; Z49260; CAA89253.1; -; Genomic_DNA.
DR EMBL; AY693104; AAT93123.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10170.1; -; Genomic_DNA.
DR PIR; S54482; S54482.
DR RefSeq; NP_013997.1; NM_001182777.1.
DR AlphaFoldDB; P53437; -.
DR BioGRID; 35448; 124.
DR ComplexPortal; CPX-1101; RNA polymerase I upstream activating factor complex.
DR DIP; DIP-1596N; -.
DR IntAct; P53437; 11.
DR MINT; P53437; -.
DR STRING; 4932.YMR270C; -.
DR MaxQB; P53437; -.
DR PaxDb; P53437; -.
DR PRIDE; P53437; -.
DR EnsemblFungi; YMR270C_mRNA; YMR270C; YMR270C.
DR GeneID; 855312; -.
DR KEGG; sce:YMR270C; -.
DR SGD; S000004883; RRN9.
DR VEuPathDB; FungiDB:YMR270C; -.
DR eggNOG; ENOG502QRG3; Eukaryota.
DR HOGENOM; CLU_061609_0_0_1; -.
DR InParanoid; P53437; -.
DR OMA; ISRGCEM; -.
DR BioCyc; YEAST:G3O-32943-MON; -.
DR PRO; PR:P53437; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; P53437; protein.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0000500; C:RNA polymerase I upstream activating factor complex; IDA:SGD.
DR GO; GO:0001181; F:RNA polymerase I general transcription initiation factor activity; IDA:SGD.
DR GO; GO:0017025; F:TBP-class protein binding; IDA:SGD.
DR GO; GO:0042790; P:nucleolar large rRNA transcription by RNA polymerase I; IDA:SGD.
DR GO; GO:0045943; P:positive regulation of transcription by RNA polymerase I; IDA:ComplexPortal.
DR InterPro; IPR019622; Rrn9_dom.
DR Pfam; PF10680; RRN9; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..365
FT /note="RNA polymerase I-specific transcription initiation
FT factor RRN9"
FT /id="PRO_0000097451"
FT CONFLICT 255
FT /note="Y -> H (in Ref. 4; AAT93123)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 365 AA; 42813 MW; 4C1E261C6FCAF850 CRC64;
MSDLDEESQI ETQIDAPIED IIRGSELTTT TADKETLKSA NELLDSLEHS HRVDLSLHLY
SAYLLKRLLY KANEKKHFYE VNQFVKTQIK DNWTSWPNPN TIIDPSVDKL YEDIPEGIAN
VSVQPGEISN RALMHASDMM RVELDAQWQK FLSKSALDHD VTLDVDELNI PNEISRNILV
KLDSLFEGLH DKIAKENEFD VRQDKHSNNI RANQIDDEPM QANRRIKYTY HDLVSRGCEM
NEDMTDIYMK SLELYNDIPE KYKKRKFRLP KQILKKYHQP KKTSSYLKEL LSKTREDFIP
VEKLLKDKRL TSKDKSKLQR LNREETEDAL NKRTFFQVKG YLEDENEISD YELDDCLIEL
PNGNI
