RRP14_YEAST
ID RRP14_YEAST Reviewed; 434 AA.
AC P36080; D6VXK5;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Ribosomal RNA-processing protein 14;
DE AltName: Full=Ribosome biogenesis protein RRP14;
GN Name=RRP14; OrderedLocusNames=YKL082C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=16544271; DOI=10.1002/yea.1353;
RA Wade C.H., Umbarger M.A., McAlear M.A.;
RT "The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains
RT over 200 genes.";
RL Yeast 23:293-306(2006).
RN [4]
RP FUNCTION, AND IDENTIFICATION IN THE 60S PRE-RIBOSOMAL PARTICLE.
RX PubMed=17272295; DOI=10.1093/nar/gkl824;
RA Oeffinger M., Fatica A., Rout M.P., Tollervey D.;
RT "Yeast Rrp14p is required for ribosomal subunit synthesis and for correct
RT positioning of the mitotic spindle during mitosis.";
RL Nucleic Acids Res. 35:1354-1366(2007).
RN [5]
RP SUBCELLULAR LOCATION, FUNCTION, AND IDENTIFICATION IN THE 90S PRE-RIBOSOMAL
RP PARTICLE.
RX PubMed=17804645; DOI=10.1261/rna.553807;
RA Yamada H., Horigome C., Okada T., Shirai C., Mizuta K.;
RT "Yeast Rrp14p is a nucleolar protein involved in both ribosome biogenesis
RT and cell polarity.";
RL RNA 13:1977-1987(2007).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Involved in ribosome biogenesis and cell polarity. Required
CC for the synthesis of both 40S and 60S ribosomal subunits and may also
CC play some direct role in correct positioning of the mitotic spindle
CC during mitosis. {ECO:0000269|PubMed:16544271,
CC ECO:0000269|PubMed:17272295, ECO:0000269|PubMed:17804645}.
CC -!- SUBUNIT: Component of the 90S and 60S pre-ribosomal particles.
CC {ECO:0000269|PubMed:17272295, ECO:0000269|PubMed:17804645}.
CC -!- INTERACTION:
CC P36080; P43586: LOC1; NbExp=3; IntAct=EBI-26762, EBI-22906;
CC P36080; P37838: NOP4; NbExp=3; IntAct=EBI-26762, EBI-12122;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:17804645}.
CC -!- SIMILARITY: Belongs to the SURF6 family. {ECO:0000305}.
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DR EMBL; Z28082; CAA81921.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09075.1; -; Genomic_DNA.
DR PIR; S37907; S37907.
DR RefSeq; NP_012841.1; NM_001179648.1.
DR PDB; 6C0F; EM; 3.70 A; 8=1-434.
DR PDBsum; 6C0F; -.
DR AlphaFoldDB; P36080; -.
DR SMR; P36080; -.
DR BioGRID; 34050; 131.
DR DIP; DIP-2694N; -.
DR IntAct; P36080; 20.
DR MINT; P36080; -.
DR STRING; 4932.YKL082C; -.
DR iPTMnet; P36080; -.
DR MaxQB; P36080; -.
DR PaxDb; P36080; -.
DR PRIDE; P36080; -.
DR EnsemblFungi; YKL082C_mRNA; YKL082C; YKL082C.
DR GeneID; 853780; -.
DR KEGG; sce:YKL082C; -.
DR SGD; S000001565; RRP14.
DR VEuPathDB; FungiDB:YKL082C; -.
DR eggNOG; KOG2885; Eukaryota.
DR GeneTree; ENSGT00390000006980; -.
DR HOGENOM; CLU_018300_0_0_1; -.
DR InParanoid; P36080; -.
DR OMA; QKKRTDN; -.
DR BioCyc; YEAST:G3O-31877-MON; -.
DR PRO; PR:P36080; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36080; protein.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; IMP:SGD.
DR GO; GO:0042274; P:ribosomal small subunit biogenesis; IMP:SGD.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR DisProt; DP02355; -.
DR InterPro; IPR029190; Rrp14/SURF6_C.
DR InterPro; IPR029188; Rrp14_N.
DR InterPro; IPR007019; SURF6.
DR PANTHER; PTHR14369; PTHR14369; 1.
DR Pfam; PF15459; RRP14; 1.
DR Pfam; PF04935; SURF6; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Coiled coil; Nucleus; Reference proteome;
KW Ribosome biogenesis; rRNA processing.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..434
FT /note="Ribosomal RNA-processing protein 14"
FT /id="PRO_0000203167"
FT REGION 32..257
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 375..434
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 162..230
FT /evidence="ECO:0000255"
FT COILED 293..360
FT /evidence="ECO:0000255"
FT COMPBIAS 32..109
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 118..135
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 136..223
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 224..238
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 239..257
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 375..392
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22814378"
SQ SEQUENCE 434 AA; 50482 MW; BCEDE9F10A466E6E CRC64;
MSNSLEERLR ANSSAFDGLL ALIPAKYYYD EKSQEQWKAK KKTKEQSKND KLKKLDPEQR
DDETSSTLEV MKKKEKDAKP VVLPGEKFKH MKMQKQKEAT SKVEGDSDLN VEVNDPMIIA
PDEDEEEEED IKVIFDDEGN EIPLESKKDT TEPDRSVEKK SITEEEKLQR KKNLEALRSK
LQAKISDMKS KRKAPGSREA ILAQRKRKEE LKKRKRLESE QEQDQDEIAS DSDMEDIDSD
LENNSKKRFK KGKKDSEINA DGVMFQNIIF DDGARATSDL QRLRKAGRTK GPAKNDVKSH
LKLLEAKKNK MEAKDELEQI KQKEKEKWQK AMLQAEGIKI RDDEKLLRKA IKRKEAQKRK
SAIEWSERKR VVEDTISERQ KRREENLRIR KDNKGKKRNK QEKMKRKYVG SAVPKKRAGF
EGRLKTGKKK GGPK
