RRP42_METTH
ID RRP42_METTH Reviewed; 271 AA.
AC O26778;
DT 25-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Exosome complex component Rrp42 {ECO:0000255|HAMAP-Rule:MF_00622};
GN Name=rrp42 {ECO:0000255|HAMAP-Rule:MF_00622}; OrderedLocusNames=MTH_682;
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) IN COMPLEX WITH RRP41.
RX PubMed=20445227; DOI=10.1107/s0907444910002908;
RA Ng C.L., Waterman D.G., Antson A.A., Ortiz-Lombardia M.;
RT "Structure of the Methanothermobacter thermautotrophicus exosome RNase PH
RT ring.";
RL Acta Crystallogr. D 66:522-528(2010).
CC -!- FUNCTION: Non-catalytic component of the exosome, which is a complex
CC involved in RNA degradation. Contributes to the structuring of the
CC Rrp41 active site. {ECO:0000255|HAMAP-Rule:MF_00622}.
CC -!- SUBUNIT: Component of the archaeal exosome complex. Forms a hexameric
CC ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The
CC hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits.
CC {ECO:0000255|HAMAP-Rule:MF_00622}.
CC -!- INTERACTION:
CC O26778; O26779: rrp41; NbExp=2; IntAct=EBI-764215, EBI-764220;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00622}.
CC -!- SIMILARITY: Belongs to the RNase PH family. Rrp42 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00622}.
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DR EMBL; AE000666; AAB85187.1; -; Genomic_DNA.
DR PIR; H69190; H69190.
DR RefSeq; WP_010876321.1; NC_000916.1.
DR PDB; 2WNR; X-ray; 2.65 A; A/C/E=1-271.
DR PDBsum; 2WNR; -.
DR AlphaFoldDB; O26778; -.
DR SMR; O26778; -.
DR DIP; DIP-33664N; -.
DR IntAct; O26778; 2.
DR STRING; 187420.MTH_682; -.
DR EnsemblBacteria; AAB85187; AAB85187; MTH_682.
DR GeneID; 24853824; -.
DR KEGG; mth:MTH_682; -.
DR PATRIC; fig|187420.15.peg.663; -.
DR HOGENOM; CLU_038194_0_0_2; -.
DR OMA; PESVFEM; -.
DR EvolutionaryTrace; O26778; -.
DR Proteomes; UP000005223; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000178; C:exosome (RNase complex); IEA:UniProtKB-KW.
DR GO; GO:0006401; P:RNA catabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd11365; RNase_PH_archRRP42; 1.
DR Gene3D; 3.30.230.70; -; 1.
DR HAMAP; MF_00622; Exosome_Rrp42; 1.
DR InterPro; IPR001247; ExoRNase_PH_dom1.
DR InterPro; IPR015847; ExoRNase_PH_dom2.
DR InterPro; IPR036345; ExoRNase_PH_dom2_sf.
DR InterPro; IPR027408; PNPase/RNase_PH_dom_sf.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR020869; Rrp42_archaea.
DR Pfam; PF01138; RNase_PH; 1.
DR Pfam; PF03725; RNase_PH_C; 1.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF55666; SSF55666; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Exosome; Reference proteome.
FT CHAIN 1..271
FT /note="Exosome complex component Rrp42"
FT /id="PRO_0000140000"
FT HELIX 11..21
FT /evidence="ECO:0007829|PDB:2WNR"
FT STRAND 37..42
FT /evidence="ECO:0007829|PDB:2WNR"
FT STRAND 47..55
FT /evidence="ECO:0007829|PDB:2WNR"
FT STRAND 58..69
FT /evidence="ECO:0007829|PDB:2WNR"
FT STRAND 80..87
FT /evidence="ECO:0007829|PDB:2WNR"
FT HELIX 89..91
FT /evidence="ECO:0007829|PDB:2WNR"
FT HELIX 102..117
FT /evidence="ECO:0007829|PDB:2WNR"
FT HELIX 123..126
FT /evidence="ECO:0007829|PDB:2WNR"
FT STRAND 127..129
FT /evidence="ECO:0007829|PDB:2WNR"
FT TURN 130..132
FT /evidence="ECO:0007829|PDB:2WNR"
FT STRAND 133..145
FT /evidence="ECO:0007829|PDB:2WNR"
FT HELIX 150..164
FT /evidence="ECO:0007829|PDB:2WNR"
FT STRAND 166..173
FT /evidence="ECO:0007829|PDB:2WNR"
FT STRAND 176..186
FT /evidence="ECO:0007829|PDB:2WNR"
FT STRAND 193..201
FT /evidence="ECO:0007829|PDB:2WNR"
FT STRAND 204..208
FT /evidence="ECO:0007829|PDB:2WNR"
FT HELIX 211..214
FT /evidence="ECO:0007829|PDB:2WNR"
FT STRAND 218..226
FT /evidence="ECO:0007829|PDB:2WNR"
FT STRAND 231..240
FT /evidence="ECO:0007829|PDB:2WNR"
FT HELIX 244..268
FT /evidence="ECO:0007829|PDB:2WNR"
SQ SEQUENCE 271 AA; 29893 MW; B5B014A289EC8381 CRC64;
MVNKMDIIPE ITRKSITDLI NNKERIDGRS LHEFRDISIE TGVISKAEGS SRVKLGNTQI
IVGVKPQIGE PFPDTPEMGV ILTNSELLPM ASPTFEPGPP DERSVELSRV VDRCIRESRM
IDLEKLCIIE GSKVWMLFLD LHIIDYDGNL FDAAVLATVA ALLDTRIPAA EVEDGEVVIN
REKMQPLPVN RKALMCTFAK IGNEIVLDPS LEEEDILTAR ISIGVTEEGS ICAMQKGGEG
PLTRDDVLKA VSIAVEKVPQ LIEYLDKSMT P
