RRP8_ARATH
ID RRP8_ARATH Reviewed; 287 AA.
AC Q84JC0; Q9FM44;
DT 15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Ribosomal RNA-processing protein 8;
DE EC=2.1.1.-;
GN OrderedLocusNames=At5g40530; ORFNames=MNF13.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Probable methyltransferase required to silence rDNA.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q84JC0-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RRP8 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08523.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB009052; BAB08523.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED94560.1; -; Genomic_DNA.
DR EMBL; BT002940; AAO22753.1; -; mRNA.
DR EMBL; BT004445; AAO42439.1; -; mRNA.
DR RefSeq; NP_001330531.1; NM_001344336.1.
DR RefSeq; NP_198869.2; NM_123417.4. [Q84JC0-1]
DR AlphaFoldDB; Q84JC0; -.
DR SMR; Q84JC0; -.
DR STRING; 3702.AT5G40530.2; -.
DR PRIDE; Q84JC0; -.
DR EnsemblPlants; AT5G40530.1; AT5G40530.1; AT5G40530. [Q84JC0-1]
DR GeneID; 834051; -.
DR Gramene; AT5G40530.1; AT5G40530.1; AT5G40530. [Q84JC0-1]
DR KEGG; ath:AT5G40530; -.
DR Araport; AT5G40530; -.
DR eggNOG; KOG3045; Eukaryota.
DR HOGENOM; CLU_027694_1_2_1; -.
DR InParanoid; Q84JC0; -.
DR OMA; KWPTNPL; -.
DR OrthoDB; 962356at2759; -.
DR PhylomeDB; Q84JC0; -.
DR PRO; PR:Q84JC0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q84JC0; baseline and differential.
DR Genevisible; Q84JC0; AT.
DR GO; GO:0005677; C:chromatin silencing complex; ISS:UniProtKB.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0033553; C:rDNA heterochromatin; ISS:UniProtKB.
DR GO; GO:0035064; F:methylated histone binding; ISS:UniProtKB.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0000183; P:rDNA heterochromatin assembly; ISS:UniProtKB.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.2150; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR007823; RRP8.
DR InterPro; IPR042036; RRP8_N.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR12787; PTHR12787; 1.
DR Pfam; PF05148; Methyltransf_8; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chromatin regulator; Methyltransferase; Nucleus;
KW Reference proteome; Repressor; rRNA processing; S-adenosyl-L-methionine;
KW Transcription; Transcription regulation; Transferase.
FT CHAIN 1..287
FT /note="Ribosomal RNA-processing protein 8"
FT /id="PRO_0000390459"
FT REGION 1..62
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..45
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 107
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:O43159"
FT BINDING 142
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:O43159"
FT BINDING 160
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:O43159"
FT BINDING 172
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:O43159"
FT BINDING 173
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:O43159"
FT BINDING 189
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:O43159"
SQ SEQUENCE 287 AA; 32760 MW; D108B51393E964DE CRC64;
MTTEENKTSR NRKRKRQRNP KPSKEEPIET TPKNQNEKKN QRDTKNQQHG GSSAPSKRPK
PSNFLDALRE RLSGGQFRML NEKLYTCSGK EALDYFKEDP QMFDMYHTGY QQQMSNWPEL
PVNSIINWLL SNSSSLVVAD FGCGDARIAK SVKNKVFSFD LVSKNPSVIA CDMSNTSLES
SSVDVAVFCL SLMGTNYSSY IKEAHRVLRP SGMLLIAEVK SRFDPNNGGA DPKDFVKAVC
DLGFTSVLKD FSNKMFILFH FKKKEQMNSN QKIIKWPELK ACLYKRR
