RRS1B_ARATH
ID RRS1B_ARATH Reviewed; 1372 AA.
AC Q9FL92;
DT 11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Disease resistance protein RRS1B {ECO:0000303|PubMed:25744164};
DE AltName: Full=Probable WRKY transcription factor 16;
DE AltName: Full=WRKY DNA-binding protein 16;
GN Name=RRS1B {ECO:0000303|PubMed:25744164}; Synonyms=WRKY16;
GN OrderedLocusNames=At5g45050; ORFNames=K21C13.24;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, INTERACTION WITH RPS4B, SUBUNIT, AND DOMAIN.
RX PubMed=25744164; DOI=10.1038/ncomms7338;
RA Saucet S.B., Ma Y., Sarris P.F., Furzer O.J., Sohn K.H., Jones J.D.;
RT "Two linked pairs of Arabidopsis TNL resistance genes independently confer
RT recognition of bacterial effector AvrRps4.";
RL Nat. Commun. 6:6338-6338(2015).
CC -!- FUNCTION: Transcription factor. Interacts specifically with the W box
CC (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-
CC acting element (By similarity). Acts also as a disease resistance
CC protein that specifically recognizes the AvrRps4 type III effector
CC avirulence protein from P.syringae. Heterodimerization with RPS4B is
CC required to form a functional complex to recognize AvrRps4 and to
CC mediate the hypersensitive response (PubMed:25744164).
CC {ECO:0000250|UniProtKB:P0DKH5, ECO:0000269|PubMed:25744164}.
CC -!- SUBUNIT: Interacts with RPS4B. RPS4B-RRS1B heterodimer interacts with
CC the bacterial effectors AvrRps4 and PopP2.
CC {ECO:0000269|PubMed:25744164}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9FL92-1; Sequence=Displayed;
CC -!- DOMAIN: The TIR domain is a signaling domain involved in cell death
CC induction. It is involved in heterodimerization of RPS4B with RRS1B,
CC but other domains also contribute to the interaction.
CC {ECO:0000269|PubMed:25744164}.
CC -!- SIMILARITY: Belongs to the disease resistance TIR-NB-LRR family.
CC {ECO:0000305}.
CC -!- WEB RESOURCE: Name=NIB-LRRS; Note=Functional and comparative genomics
CC of disease resistance gene homologs;
CC URL="http://niblrrs.ucdavis.edu";
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DR EMBL; AB010693; BAB10888.1; -; Genomic_DNA.
DR EMBL; AB019224; BAB10888.1; JOINED; Genomic_DNA.
DR EMBL; CP002688; AED95194.1; -; Genomic_DNA.
DR RefSeq; NP_851133.1; NM_180802.2. [Q9FL92-1]
DR AlphaFoldDB; Q9FL92; -.
DR SMR; Q9FL92; -.
DR BioGRID; 19785; 1.
DR IntAct; Q9FL92; 1.
DR STRING; 3702.AT5G45050.1; -.
DR iPTMnet; Q9FL92; -.
DR PaxDb; Q9FL92; -.
DR EnsemblPlants; AT5G45050.1; AT5G45050.1; AT5G45050. [Q9FL92-1]
DR GeneID; 834536; -.
DR Gramene; AT5G45050.1; AT5G45050.1; AT5G45050. [Q9FL92-1]
DR KEGG; ath:AT5G45050; -.
DR Araport; AT5G45050; -.
DR TAIR; locus:2155347; AT5G45050.
DR HOGENOM; CLU_001561_0_3_1; -.
DR InParanoid; Q9FL92; -.
DR OrthoDB; 100821at2759; -.
DR PhylomeDB; Q9FL92; -.
DR PRO; PR:Q9FL92; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FL92; baseline and differential.
DR Genevisible; Q9FL92; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0002758; P:innate immune response-activating signal transduction; IDA:UniProtKB.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 2.20.25.80; -; 1.
DR Gene3D; 3.40.50.10140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR011713; Leu-rich_rpt_3.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000157; TIR_dom.
DR InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR InterPro; IPR003657; WRKY_dom.
DR InterPro; IPR036576; WRKY_dom_sf.
DR PANTHER; PTHR11017; PTHR11017; 1.
DR Pfam; PF07725; LRR_3; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF03106; WRKY; 1.
DR SMART; SM00774; WRKY; 1.
DR SUPFAM; SSF118290; SSF118290; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF52200; SSF52200; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50104; TIR; 1.
DR PROSITE; PS50811; WRKY; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; DNA-binding; Leucine-rich repeat;
KW Nucleotide-binding; Nucleus; Plant defense; Reference proteome; Repeat;
KW Transcription; Transcription regulation.
FT CHAIN 1..1372
FT /note="Disease resistance protein RRS1B"
FT /id="PRO_0000133658"
FT DOMAIN 2..137
FT /note="TIR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT DOMAIN 166..417
FT /note="NB-ARC"
FT REPEAT 491..515
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 528..544
FT /note="LRR 2; degenerate"
FT /evidence="ECO:0000305"
FT REPEAT 545..568
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 570..591
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 614..637
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 638..658
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 659..681
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 693..718
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 723..747
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 749..767
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT REPEAT 768..792
FT /note="LRR 11"
FT /evidence="ECO:0000255"
FT REPEAT 798..823
FT /note="LRR 12"
FT /evidence="ECO:0000255"
FT DNA_BIND 1174..1240
FT /note="WRKY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00223"
FT REGION 1246..1288
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1337..1372
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 950..964
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 1246..1271
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1337..1354
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 170..177
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1372 AA; 155701 MW; E343FF0D880B9A70 CRC64;
MTESEQIVYI SCIEEVRYSF VSHLSKALQR KGVNDVFIDS DDSLSNESQS MVERARVSVM
ILPGNRTVSL DKLVKVLDCQ KNKDQVVVPV LYGVRSSETE WLSALDSKGF SSVHHSRKEC
SDSQLVKETV RDVYEKLFYM ERIGIYSKLL EIEKMINKQP LDIRCVGIWG MPGIGKTTLA
KAVFDQMSGE FDAHCFIEDY TKAIQEKGVY CLLEEQFLKE NAGASGTVTK LSLLRDRLNN
KRVLVVLDDV RSPLVVESFL GGFDWFGPKS LIIITSKDKS VFRLCRVNQI YEVQGLNEKE
ALQLFSLCAS IDDMAEQNLH EVSMKVIKYA NGHPLALNLY GRELMGKKRP PEMEIAFLKL
KECPPAIFVD AIKSSYDTLN DREKNIFLDI ACFFQGENVD YVMQLLEGCG FFPHVGIDVL
VEKSLVTISE NRVRMHNLIQ DVGRQIINRE TRQTKRRSRL WEPCSIKYLL EDKEQNENEE
QKTTFERAQV PEEIEGMFLD TSNLSFDIKH VAFDNMLNLR LFKIYSSNPE VHHVNNFLKG
SLSSLPNVLR LLHWENYPLQ FLPQNFDPIH LVEINMPYSQ LKKLWGGTKD LEMLKTIRLC
HSQQLVDIDD LLKAQNLEVV DLQGCTRLQS FPATGQLLHL RVVNLSGCTE IKSFPEIPPN
IETLNLQGTG IIELPLSIVK PNYRELLNLL AEIPGLSGVS NLEQSDLKPL TSLMKISTSY
QNPGKLSCLE LNDCSRLRSL PNMVNLELLK ALDLSGCSEL ETIQGFPRNL KELYLVGTAV
RQVPQLPQSL EFFNAHGCVS LKSIRLDFKK LPVHYTFSNC FDLSPQVVND FLVQAMANVI
AKHIPRERHV TGFSQKTVQR SSRDSQQELN KTLAFSFCAP SHANQNSKLD LQPGSSSMTR
LDPSWRNTLV GFAMLVQVAF SEGYCDDTDF GISCVCKWKN KEGHSHRREI NLHCWALGKA
VERDHTFVFF DVNMRPDTDE GNDPDIWADL VVFEFFPVNK QRKPLNDSCT VTRCGVRLIT
AVNCNTSIEN ISPVLSLDPM EVSGNEDEEV LRVRYAGLQE IYKALFLYIA GLFNDEDVGL
VAPLIANIID MDVSYGLKVL AYRSLIRVSS NGEIVMHYLL RQMGKEILHT ESKKTDKLVD
NIQSSMIATK EIEITRSKSR RKNNKEKRVV CVVDRGSRSS DLWVWRKYGQ KPIKSSPYPR
SYYRCASSKG CFARKQVERS RTDPNVSVIT YISEHNHPFP TLRNTLAGST RSSSSKCSDV
TTSASSTVSQ DKEGPDKSHL PSSPASPPYA AMVVKEEDME QWDNMEFDVD VEEDTFIPEL
FPEDTFADMD KLEENSQTMF LSRRSSGGNM EAQGKNSSDD REVNLPSKIL NR
