AXE1_ASPFU
ID AXE1_ASPFU Reviewed; 371 AA.
AC Q4WBW4;
DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Probable acetylxylan esterase A;
DE EC=3.1.1.72;
DE Flags: Precursor;
GN Name=axeA; Synonyms=aceA; ORFNames=AFUA_8G06570;
OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS A1100) (Aspergillus fumigatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=330879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX PubMed=16372009; DOI=10.1038/nature04332;
RA Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA Barrell B.G., Denning D.W.;
RT "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT Aspergillus fumigatus.";
RL Nature 438:1151-1156(2005).
CC -!- FUNCTION: Acetylxylan esterase involved in the hydrolysis of xylan, a
CC major structural heterogeneous polysaccharide found in plant biomass
CC representing the second most abundant polysaccharide in the biosphere,
CC after cellulose. Degrades acetylated xylans by cleaving acetyl side
CC groups from the hetero-xylan backbone (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Deacetylation of xylans and xylo-oligosaccharides.;
CC EC=3.1.1.72;
CC -!- PATHWAY: Glycan degradation; xylan degradation.
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- DOMAIN: Has a modular structure: a carbohydrate esterase catalytic
CC module at the N-terminus, a linker rich in serines and threonines, and
CC a C-terminal carbohydrate-binding module (CBM). The genes for catalytic
CC modules and CBMs seem to have evolved separately and have been linked
CC by gene fusion.
CC -!- SIMILARITY: Belongs to the carbohydrate esterase 1 (CE1) family. AxeA
CC subfamily. {ECO:0000305}.
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DR EMBL; AAHF01000013; EAL85420.1; -; Genomic_DNA.
DR RefSeq; XP_747458.1; XM_742365.1.
DR AlphaFoldDB; Q4WBW4; -.
DR SMR; Q4WBW4; -.
DR STRING; 746128.CADAFUBP00007907; -.
DR ESTHER; aspfu-axe1; Esterase_phb.
DR EnsemblFungi; EAL85420; EAL85420; AFUA_8G06570.
DR GeneID; 3504738; -.
DR KEGG; afm:AFUA_8G06570; -.
DR VEuPathDB; FungiDB:Afu8g06570; -.
DR eggNOG; ENOG502QTDU; Eukaryota.
DR HOGENOM; CLU_027551_1_1_1; -.
DR InParanoid; Q4WBW4; -.
DR OMA; IANMVKW; -.
DR OrthoDB; 1169544at2759; -.
DR UniPathway; UPA00114; -.
DR Proteomes; UP000002530; Chromosome 8.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0046555; F:acetylxylan esterase activity; IEA:UniProtKB-EC.
DR GO; GO:0030248; F:cellulose binding; IEA:InterPro.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0045493; P:xylan catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000254; Cellulose-bd_dom_fun.
DR InterPro; IPR010126; Esterase_phb.
DR Pfam; PF00734; CBM_1; 1.
DR Pfam; PF10503; Esterase_PHB; 1.
DR SMART; SM00236; fCBD; 1.
DR SUPFAM; SSF53474; SSF53474; 2.
DR TIGRFAMs; TIGR01840; esterase_phb; 1.
DR PROSITE; PS00562; CBM1_1; 1.
DR PROSITE; PS51164; CBM1_2; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Cellulose degradation; Glycoprotein; Hydrolase;
KW Polysaccharide degradation; Reference proteome; Secreted; Serine esterase;
KW Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..371
FT /note="Probable acetylxylan esterase A"
FT /id="PRO_0000393479"
FT DOMAIN 335..371
FT /note="CBM1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00597"
FT REGION 32..304
FT /note="Catalytic"
FT REGION 305..336
FT /note="Ser/Thr-rich linker"
FT REGION 305..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 149
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT CARBOHYD 191
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 371 AA; 39252 MW; 66AA089FB0675CC9 CRC64;
MRALSVFVAL FSFLALSSAS PGQDVAKRVT SGSLQQVTNF GSNPSGTLMY IYVPNNLATK
PGIVVAIHYC TGTAQAYYTG SPYAQLAEKY GFIVIYPQSP YSGTCWDVSS QSALTHNGGG
DSNSIANMVT WTISQYNADT SKVFVTGSSS GAMMTNVMAA TYPELFAAAT VYSGVPAGCF
YSSSNQVNGW NSSCAQGNVI STPEVWGGIA KAMYPGYTGP RPRMQIYHGS VDTTLYPQNY
YETCKQWAGV FGYNYNSPQS TQSNTPQANY QTTIWGPNLQ GIFATGVGHT VPIHGEQDME
WFGFTGGSSS TTTTATTPPT TSTTTSSGGS STSTGVAEHW GQCGGNGWTG PTACASGYTC
TVINEWYSQC L
