AXS_ORYSJ
ID AXS_ORYSJ Reviewed; 398 AA.
AC Q8S9Z2; A0A0P0VDB6;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=UDP-D-apiose/UDP-D-xylose synthase;
GN OrderedLocusNames=Os01g0969100, LOC_Os01g73790;
GN ORFNames=OJ1656_A11.20, OsJ_04912;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Catalyzes the conversion of UDP-D-glucuronate to a mixture of
CC UDP-D-apiose and UDP-D-xylose. D-Apiose (3-C-hydroxymethyl-d-erythrose)
CC is the only plant cell wall monosaccharide with a branched carbon
CC skeleton and found in rhamnogalacturonan II (RG-II), apiogalacturonan,
CC and several apioglycosides (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. {ECO:0000305}.
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DR EMBL; AP003448; BAB85329.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF07437.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS76414.1; -; Genomic_DNA.
DR EMBL; CM000138; EEE56081.1; -; Genomic_DNA.
DR EMBL; AK070623; BAG92063.1; -; mRNA.
DR RefSeq; XP_015625150.1; XM_015769664.1.
DR AlphaFoldDB; Q8S9Z2; -.
DR SMR; Q8S9Z2; -.
DR STRING; 4530.OS01T0969100-01; -.
DR PaxDb; Q8S9Z2; -.
DR PRIDE; Q8S9Z2; -.
DR EnsemblPlants; Os01t0969100-01; Os01t0969100-01; Os01g0969100.
DR GeneID; 4323835; -.
DR Gramene; Os01t0969100-01; Os01t0969100-01; Os01g0969100.
DR KEGG; osa:4323835; -.
DR eggNOG; KOG1429; Eukaryota.
DR HOGENOM; CLU_007383_7_0_1; -.
DR InParanoid; Q8S9Z2; -.
DR OMA; WIYSCAK; -.
DR OrthoDB; 848823at2759; -.
DR PlantReactome; R-OSA-1119563; UDP-D-xylose biosynthesis.
DR PlantReactome; R-OSA-1119574; UDP-L-arabinose biosynthesis and transport.
DR PlantReactome; R-OSA-5654894; UDP-D-apiose biosynthesis.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000007752; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q8S9Z2; OS.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016831; F:carboxy-lyase activity; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR CDD; cd05257; Arna_like_SDR_e; 1.
DR InterPro; IPR045869; Arna-like_SDR_e.
DR InterPro; IPR001509; Epimerase_deHydtase.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF01370; Epimerase; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Cell wall biogenesis/degradation; Cytoplasm; NAD; Reference proteome.
FT CHAIN 1..398
FT /note="UDP-D-apiose/UDP-D-xylose synthase"
FT /id="PRO_0000423719"
FT ACT_SITE 193
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 57..88
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 190
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 193..197
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 222
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 243
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 244..248
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 261..268
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 345..349
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 398 AA; 44333 MW; 37448119B2DD7879 CRC64;
MSSSSSPPAA SAAARLDLDG NPIAPLTICM IGAGGFIGSH LCEKLMAETA HVVYAVDVYC
DKIRHLVDPA PPHLHGRISF HRLNIKNDSR LEGLIKMADL TINLAAICTP ADYNTRPLDT
IYSNFIDALP VVKYCSENNK RLIHFSTCEV YGKTIGSFLP TDHPLRKEPE FYVLKEDESP
CIFGPIVKQR WSYACAKQLI ERLIFAEGAE NGLEFTIVRP FNWIGPRMDF IPGVDGPSEG
VPRVLACFSN NLLRREPLKL VDGGQSQRTF VYIKDAIEAV HLMIENPARA NGQIFNVGNP
NNEVTVRQLA EMMTEVYANV SGEPPLDEPM IDVSSKQFYG EGYDDSDKRI PDMTIINKQL
GWNPKTPLKD LLETTLTYQH KTYKEAIKRQ MSQASASS
