AXYLT_ARATH
ID AXYLT_ARATH Reviewed; 468 AA.
AC Q9LVW3;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Anthocyanidin 3-O-glucoside 2'''-O-xylosyltransferase;
DE Short=A3G2''XylT;
DE EC=2.4.2.51;
GN Name=A3G2XYLT; Synonyms=UF3GT, UFGT, UGT79B1; OrderedLocusNames=At5g54060;
GN ORFNames=MJP23.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA De Los Reyes C., Quan R., Chen H., Bautista V.R., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=11042215; DOI=10.1074/jbc.m007447200;
RA Li Y., Baldauf S., Lim E.K., Bowles D.J.;
RT "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of
RT Arabidopsis thaliana.";
RL J. Biol. Chem. 276:4338-4343(2001).
RN [5]
RP INDUCTION BY SUCROSE.
RX PubMed=16384906; DOI=10.1104/pp.105.072579;
RA Solfanelli C., Poggi A., Loreti E., Alpi A., Perata P.;
RT "Sucrose-specific induction of the anthocyanin biosynthetic pathway in
RT Arabidopsis.";
RL Plant Physiol. 140:637-646(2006).
RN [6]
RP INDUCTION BY JASMONIC ACID.
RX PubMed=19596700; DOI=10.1093/jxb/erp223;
RA Shan X., Zhang Y., Peng W., Wang Z., Xie D.;
RT "Molecular mechanism for jasmonate-induction of anthocyanin accumulation in
RT Arabidopsis.";
RL J. Exp. Bot. 60:3849-3860(2009).
RN [7]
RP INDUCTION BY JASMONIC ACID.
RX PubMed=21545406; DOI=10.1111/j.1744-7909.2011.01042.x;
RA Peng Z., Han C., Yuan L., Zhang K., Huang H., Ren C.;
RT "Brassinosteroid enhances jasmonate-induced anthocyanin accumulation in
RT Arabidopsis seedlings.";
RL J. Integr. Plant Biol. 53:632-640(2011).
RN [8]
RP INDUCTION BY CYTOKININ.
RX PubMed=22699753; DOI=10.1007/s10059-012-0114-2;
RA Das P.K., Shin D.H., Choi S.B., Yoo S.D., Choi G., Park Y.I.;
RT "Cytokinins enhance sugar-induced anthocyanin biosynthesis in
RT Arabidopsis.";
RL Mol. Cells 34:93-101(2012).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, AND CATALYTIC ACTIVITY.
RX PubMed=21899608; DOI=10.1111/j.1365-313x.2011.04779.x;
RA Yonekura-Sakakibara K., Fukushima A., Nakabayashi R., Hanada K.,
RA Matsuda F., Sugawara S., Inoue E., Kuromori T., Ito T., Shinozaki K.,
RA Wangwattana B., Yamazaki M., Saito K.;
RT "Two glycosyltransferases involved in anthocyanin modification delineated
RT by transcriptome independent component analysis in Arabidopsis thaliana.";
RL Plant J. 69:154-167(2012).
RN [10]
RP REVIEW, AND NOMENCLATURE.
RX PubMed=23473981; DOI=10.1016/j.plaphy.2013.02.001;
RA Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M.,
RA Tohge T., Fernie A.R.;
RT "The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic
RT diversity.";
RL Plant Physiol. Biochem. 72:21-34(2013).
CC -!- FUNCTION: Contributes to the last few anthocyanin biosynthetic steps.
CC Converts cyanidin 3-O-glucoside to cyanidin 3-O-xylosyl(1->2)glucoside.
CC Can use 3-O-glucosylated anthocyanidins/flavonols and uridine
CC diphosphate (UDP)-xylose as substrates. {ECO:0000269|PubMed:21899608}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an anthocyanidin 3-O-beta-D-glucoside + UDP-alpha-D-xylose =
CC an anthocyanidin 3-O-beta-D-sambubioside + 2 H(+) + UDP;
CC Xref=Rhea:RHEA:35443, ChEBI:CHEBI:15378, ChEBI:CHEBI:16307,
CC ChEBI:CHEBI:57632, ChEBI:CHEBI:58223, ChEBI:CHEBI:77945; EC=2.4.2.51;
CC Evidence={ECO:0000269|PubMed:21899608};
CC -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC -!- INDUCTION: By sucrose and cytokinin. Induced by jasmonic acid (JA) in a
CC COI1- and brassinosteroids-dependent manner.
CC {ECO:0000269|PubMed:16384906, ECO:0000269|PubMed:19596700,
CC ECO:0000269|PubMed:21545406, ECO:0000269|PubMed:22699753}.
CC -!- DISRUPTION PHENOTYPE: Strong reduction in anthocyanin accumulation.
CC {ECO:0000269|PubMed:21899608}.
CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AB018115; BAA97127.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96443.1; -; Genomic_DNA.
DR EMBL; BT033073; ACE82596.1; -; mRNA.
DR RefSeq; NP_200217.1; NM_124785.3.
DR AlphaFoldDB; Q9LVW3; -.
DR SMR; Q9LVW3; -.
DR STRING; 3702.AT5G54060.1; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR PaxDb; Q9LVW3; -.
DR PRIDE; Q9LVW3; -.
DR ProteomicsDB; 241164; -.
DR EnsemblPlants; AT5G54060.1; AT5G54060.1; AT5G54060.
DR GeneID; 835489; -.
DR Gramene; AT5G54060.1; AT5G54060.1; AT5G54060.
DR KEGG; ath:AT5G54060; -.
DR Araport; AT5G54060; -.
DR TAIR; locus:2166552; AT5G54060.
DR eggNOG; KOG1192; Eukaryota.
DR HOGENOM; CLU_001724_2_3_1; -.
DR InParanoid; Q9LVW3; -.
DR OMA; FYDSAHW; -.
DR OrthoDB; 508327at2759; -.
DR PhylomeDB; Q9LVW3; -.
DR BioCyc; ARA:AT5G54060-MON; -.
DR BioCyc; MetaCyc:MON-18502; -.
DR BRENDA; 2.4.2.51; 399.
DR UniPathway; UPA00154; -.
DR PRO; PR:Q9LVW3; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LVW3; baseline and differential.
DR Genevisible; Q9LVW3; AT.
DR GO; GO:0102580; F:cyanidin 3-O-glucoside 2-O''-xylosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0035252; F:UDP-xylosyltransferase activity; IDA:TAIR.
DR GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IMP:TAIR.
DR GO; GO:0071368; P:cellular response to cytokinin stimulus; IEP:UniProtKB.
DR GO; GO:0071395; P:cellular response to jasmonic acid stimulus; IEP:UniProtKB.
DR GO; GO:1901038; P:cyanidin 3-O-glucoside metabolic process; IMP:TAIR.
DR GO; GO:0042732; P:D-xylose metabolic process; IEA:UniProtKB-KW.
DR CDD; cd03784; GT1_Gtf-like; 1.
DR InterPro; IPR002213; UDP_glucos_trans.
DR InterPro; IPR035595; UDP_glycos_trans_CS.
DR Pfam; PF00201; UDPGT; 1.
DR PROSITE; PS00375; UDPGT; 1.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; Flavonoid biosynthesis; Reference proteome;
KW Transferase; Xylose metabolism.
FT CHAIN 1..468
FT /note="Anthocyanidin 3-O-glucoside 2'''-O-
FT xylosyltransferase"
FT /id="PRO_0000409107"
FT BINDING 284
FT /ligand="UDP-alpha-D-xylose"
FT /ligand_id="ChEBI:CHEBI:57632"
FT /evidence="ECO:0000250"
FT BINDING 344..346
FT /ligand="UDP-alpha-D-xylose"
FT /ligand_id="ChEBI:CHEBI:57632"
FT /evidence="ECO:0000250"
FT BINDING 361..369
FT /ligand="UDP-alpha-D-xylose"
FT /ligand_id="ChEBI:CHEBI:57632"
FT /evidence="ECO:0000250"
FT BINDING 383..386
FT /ligand="UDP-alpha-D-xylose"
FT /ligand_id="ChEBI:CHEBI:57632"
FT /evidence="ECO:0000250"
SQ SEQUENCE 468 AA; 52048 MW; 234E16C30AABA7AA CRC64;
MGVFGSNESS SMSIVMYPWL AFGHMTPFLH LSNKLAEKGH KIVFLLPKKA LNQLEPLNLY
PNLITFHTIS IPQVKGLPPG AETNSDVPFF LTHLLAVAMD QTRPEVETIF RTIKPDLVFY
DSAHWIPEIA KPIGAKTVCF NIVSAASIAL SLVPSAEREV IDGKEMSGEE LAKTPLGYPS
SKVVLRPHEA KSLSFVWRKH EAIGSFFDGK VTAMRNCDAI AIRTCRETEG KFCDYISRQY
SKPVYLTGPV LPGSQPNQPS LDPQWAEWLA KFNHGSVVFC AFGSQPVVNK IDQFQELCLG
LESTGFPFLV AIKPPSGVST VEEALPEGFK ERVQGRGVVF GGWIQQPLVL NHPSVGCFVS
HCGFGSMWES LMSDCQIVLV PQHGEQILNA RLMTEEMEVA VEVEREKKGW FSRQSLENAV
KSVMEEGSEI GEKVRKNHDK WRCVLTDSGF SDGYIDKFEQ NLIELVKS
