AZAK_ASPNA
ID AZAK_ASPNA Reviewed; 468 AA.
AC G3XMC9;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 25-MAY-2022, entry version 35.
DE RecName: Full=Efflux pump azaK {ECO:0000303|PubMed:22921072};
DE AltName: Full=Azaphilone biosynthesis cluster protein azaK {ECO:0000303|PubMed:22921072};
GN Name=azaK {ECO:0000303|PubMed:22921072}; ORFNames=ASPNIDRAFT_188912;
OS Aspergillus niger (strain ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 /
OS NCTC 3858a / NRRL 328 / USDA 3528.7).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=380704;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL
RC 328 / USDA 3528.7;
RX PubMed=21543515; DOI=10.1101/gr.112169.110;
RA Andersen M.R., Salazar M.P., Schaap P.J., van de Vondervoort P.J.I.,
RA Culley D., Thykaer J., Frisvad J.C., Nielsen K.F., Albang R., Albermann K.,
RA Berka R.M., Braus G.H., Braus-Stromeyer S.A., Corrochano L.M., Dai Z.,
RA van Dijck P.W.M., Hofmann G., Lasure L.L., Magnuson J.K., Menke H.,
RA Meijer M., Meijer S.L., Nielsen J.B., Nielsen M.L., van Ooyen A.J.J.,
RA Pel H.J., Poulsen L., Samson R.A., Stam H., Tsang A., van den Brink J.M.,
RA Atkins A., Aerts A., Shapiro H., Pangilinan J., Salamov A., Lou Y.,
RA Lindquist E., Lucas S., Grimwood J., Grigoriev I.V., Kubicek C.P.,
RA Martinez D., van Peij N.N.M.E., Roubos J.A., Nielsen J., Baker S.E.;
RT "Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015
RT versus enzyme-producing CBS 513.88.";
RL Genome Res. 21:885-897(2011).
RN [2]
RP FUNCTION, AND INDUCTION.
RX PubMed=22921072; DOI=10.1016/j.chembiol.2012.07.004;
RA Zabala A.O., Xu W., Chooi Y.H., Tang Y.;
RT "Characterization of a silent azaphilone gene cluster from Aspergillus
RT niger ATCC 1015 reveals a hydroxylation-mediated pyran-ring formation.";
RL Chem. Biol. 19:1049-1059(2012).
CC -!- FUNCTION: Efflux pump that might be required for efficient secretion of
CC azaphilones (PubMed:22921072). {ECO:0000269|PubMed:22921072}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Expression is under the control of the azaphilone cluster-
CC specific transcription factor azaR (PubMed:22921072).
CC {ECO:0000269|PubMed:22921072}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; ACJE01000001; EHA28242.1; -; Genomic_DNA.
DR AlphaFoldDB; G3XMC9; -.
DR SMR; G3XMC9; -.
DR EnsemblFungi; EHA28242; EHA28242; ASPNIDRAFT_188912.
DR VEuPathDB; FungiDB:ASPNIDRAFT2_1079556; -.
DR HOGENOM; CLU_001265_54_6_1; -.
DR Proteomes; UP000009038; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR001958; Tet-R_TetA/multi-R_MdtG.
DR Pfam; PF07690; MFS_1; 1.
DR PRINTS; PR01035; TCRTETA.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..468
FT /note="Efflux pump azaK"
FT /id="PRO_0000437627"
FT TRANSMEM 43..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 429..449
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 228
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 468 AA; 50044 MW; 69AB21927FE542E3 CRC64;
MTVHPPAVAD ETSPLLPSQD GPGHNGIVPA ATKPKELQSS MSQVALLCCA RAIDPLAFFT
IFPFVNQMIA DTAGIDEADV GFYSGIIESL FSVTQMMLMI PWARAADRMG RKPVLILSLA
GLSVSSALFG FSRTLGQMVF FRCLAGTFGG TVVTVRVMIS ENSTPDTQAR AFSYFSLANT
IGTVIGPLLG GALCRPGGVF RHYPYALPTV AAGAFGVTVT VACLMFVNET RKPADHTPHE
TASPTWTSAK ILRSQGVLPV LYIHGHSMML AFAYTAVSPV FYFTSPRLGG YGFSPFYISL
FLGGSGIAQT IWLVLVYPPL HKRLGTGNIL RGLCFVWIIF LAATVGASVL HRHCEMVAFW
ILAPLALVLG SSVAMQLTAM QLALDSVSPS PAALGTLNAM SLAIISFLRA VAPAMFTSMY
ASTLKLSSPG FYTFWLVLGG LVLVLAFTLR WLPEQVEKAP RKLGRSSA
