ID   AZAR_ASPNA              Reviewed;         465 AA.
AC   G3XMC5;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2016, sequence version 2.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Azaphilone cluster-specific transcription factor azaR {ECO:0000305};
DE   AltName: Full=Azaphilone biosynthesis cluster protein azaR {ECO:0000303|PubMed:22921072};
GN   Name=azaR {ECO:0000303|PubMed:22921072}; ORFNames=ASPNIDRAFT_132962;
OS   Aspergillus niger (strain ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 /
OS   NCTC 3858a / NRRL 328 / USDA 3528.7).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=380704;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL
RC   328 / USDA 3528.7;
RX   PubMed=21543515; DOI=10.1101/gr.112169.110;
RA   Andersen M.R., Salazar M.P., Schaap P.J., van de Vondervoort P.J.I.,
RA   Culley D., Thykaer J., Frisvad J.C., Nielsen K.F., Albang R., Albermann K.,
RA   Berka R.M., Braus G.H., Braus-Stromeyer S.A., Corrochano L.M., Dai Z.,
RA   van Dijck P.W.M., Hofmann G., Lasure L.L., Magnuson J.K., Menke H.,
RA   Meijer M., Meijer S.L., Nielsen J.B., Nielsen M.L., van Ooyen A.J.J.,
RA   Pel H.J., Poulsen L., Samson R.A., Stam H., Tsang A., van den Brink J.M.,
RA   Atkins A., Aerts A., Shapiro H., Pangilinan J., Salamov A., Lou Y.,
RA   Lindquist E., Lucas S., Grimwood J., Grigoriev I.V., Kubicek C.P.,
RA   Martinez D., van Peij N.N.M.E., Roubos J.A., Nielsen J., Baker S.E.;
RT   "Comparative genomics of citric-acid-producing Aspergillus niger ATCC 1015
RT   versus enzyme-producing CBS 513.88.";
RL   Genome Res. 21:885-897(2011).
RN   [2]
RP   FUNCTION.
RX   PubMed=22921072; DOI=10.1016/j.chembiol.2012.07.004;
RA   Zabala A.O., Xu W., Chooi Y.H., Tang Y.;
RT   "Characterization of a silent azaphilone gene cluster from Aspergillus
RT   niger ATCC 1015 reveals a hydroxylation-mediated pyran-ring formation.";
RL   Chem. Biol. 19:1049-1059(2012).
CC   -!- FUNCTION: Transcription factor that regulates the expression of the
CC       gene cluster that mediates the biosynthesis of azaphilones, a class of
CC       fungal metabolites characterized by a highly oxygenated pyrano-quinone
CC       bicyclic core and exhibiting a broad range of bioactivities
CC       (PubMed:22921072). {ECO:0000269|PubMed:22921072}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EHA28238.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; ACJE01000001; EHA28238.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; G3XMC5; -.
DR   STRING; 380704.G3XMC5; -.
DR   EnsemblFungi; EHA28238; EHA28238; ASPNIDRAFT_132962.
DR   HOGENOM; CLU_011017_3_1_1; -.
DR   Proteomes; UP000009038; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR007219; Transcription_factor_dom_fun.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF04082; Fungal_trans; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..465
FT                   /note="Azaphilone cluster-specific transcription factor
FT                   azaR"
FT                   /id="PRO_0000437607"
FT   DNA_BIND        27..53
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..22
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   465 AA;  52222 MW;  B52E0C3FCA9ECC81 CRC64;
     MSDSRTTTTK NNTTNHKTSR QGPGSACEEC RRRKLRCDRQ PQCQNCVDAG VYCVTNLARP
     ARGPKKGHLK ALKGRIATLE RCLLEQRDGM LVDPISDDEL LDKALSASSP ASQSPDPSEE
     VSENILEDLD QVFFERVQPM VPILQRHRYF SWAREPQQSA NRRGLQQAIR TLAAGVSSQL
     PEVRVALYRT TRHTLESLES DDTLLTAPDF EQVQAWILIA IYEFMQVSYS RGWMSAGRVF
     RLVQLLRLPE IDASPLSLLD LDLDPDSKWV VAEEKRRTVW MAYIMDCSLN LRHKGSLTLT
     EQALTRLPMP ESEFQCGHPI SMGFLAEALA GTDITSPLSS FAKCILLATI SGRTLSHRHL
     SMAELLRGNP LQDVWTRHQW IDTTLTAHLQ LSFSLPTAAE SSDPMLLFAK MIGQAGVLSL
     YDILQSTPWE PETVSLLPDY EACALQAARE TVVLARNLRH FSCFK