AZF1_ARATH
ID AZF1_ARATH Reviewed; 245 AA.
AC Q9SSW1;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Zinc finger protein AZF1;
DE AltName: Full=Zinc finger protein OZAKGYO;
DE AltName: Full=Zinc-finger protein 1;
GN Name=AZF1; Synonyms=ZF1; OrderedLocusNames=At5g67450; ORFNames=K8K14.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Columbia;
RX PubMed=10806347; DOI=10.1016/s0378-1119(00)00133-5;
RA Sakamoto H., Araki T., Meshi T., Iwabuchi M.;
RT "Expression of a subset of the Arabidopsis Cys(2)/His(2)-type zinc-finger
RT protein gene family under water stress.";
RL Gene 248:23-32(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Kim S., Choi Y., Jeong C.-W., Choi E.K., Chang M.-S., Lee I., Fischer R.L.,
RA Choi Y.D., Lee J.S.;
RT "Zinc finger protein OZAKGYO.";
RL Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=15333755; DOI=10.1104/pp.104.046599;
RA Sakamoto H., Maruyama K., Sakuma Y., Meshi T., Iwabuchi M., Shinozaki K.,
RA Yamaguchi-Shinozaki K.;
RT "Arabidopsis Cys2/His2-type zinc-finger proteins function as transcription
RT repressors under drought, cold, and high-salinity stress conditions.";
RL Plant Physiol. 136:2734-2746(2004).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=21852415; DOI=10.1104/pp.111.182683;
RA Kodaira K.S., Qin F., Tran L.S., Maruyama K., Kidokoro S., Fujita Y.,
RA Shinozaki K., Yamaguchi-Shinozaki K.;
RT "Arabidopsis Cys2/His2 zinc-finger proteins AZF1 and AZF2 negatively
RT regulate abscisic acid-repressive and auxin-inducible genes under abiotic
RT stress conditions.";
RL Plant Physiol. 157:742-756(2011).
CC -!- FUNCTION: Transcriptional repressor involved in the inhibition of plant
CC growth under abiotic stress conditions. Can repress the expression of
CC various genes, including osmotic stress and abscisic acid-repressive
CC genes and auxin-inducible genes, by binding to their promoter regions
CC in a DNA sequence-specific manner. {ECO:0000269|PubMed:15333755,
CC ECO:0000269|PubMed:21852415}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15333755,
CC ECO:0000269|PubMed:21852415}.
CC -!- TISSUE SPECIFICITY: Highly expressed in roots and at lower levels in
CC leaves and stems. {ECO:0000269|PubMed:10806347,
CC ECO:0000269|PubMed:15333755}.
CC -!- INDUCTION: By abscisic acid (ABA), ethylene, dehydration, salt and
CC cold. {ECO:0000269|PubMed:10806347, ECO:0000269|PubMed:15333755,
CC ECO:0000269|PubMed:21852415}.
CC -!- MISCELLANEOUS: Plants overexpressing AZF1 have increased sensitivity to
CC salt stress and barely survive under high salt conditons.
CC {ECO:0000305|PubMed:21852415}.
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DR EMBL; AB030731; BAA85108.1; -; Genomic_DNA.
DR EMBL; AY123328; AAM78034.1; -; Genomic_DNA.
DR EMBL; AB007645; BAB09030.1; -; Genomic_DNA.
DR EMBL; CP002688; AED98346.1; -; Genomic_DNA.
DR EMBL; BT003064; AAO23629.1; -; mRNA.
DR EMBL; AK227608; BAE99599.1; -; mRNA.
DR RefSeq; NP_201546.1; NM_126145.3.
DR AlphaFoldDB; Q9SSW1; -.
DR BioGRID; 22123; 1.
DR IntAct; Q9SSW1; 1.
DR STRING; 3702.AT5G67450.1; -.
DR PaxDb; Q9SSW1; -.
DR PRIDE; Q9SSW1; -.
DR EnsemblPlants; AT5G67450.1; AT5G67450.1; AT5G67450.
DR GeneID; 836881; -.
DR Gramene; AT5G67450.1; AT5G67450.1; AT5G67450.
DR KEGG; ath:AT5G67450; -.
DR Araport; AT5G67450; -.
DR TAIR; locus:2158192; AT5G67450.
DR eggNOG; KOG1721; Eukaryota.
DR HOGENOM; CLU_059471_1_2_1; -.
DR InParanoid; Q9SSW1; -.
DR OMA; KMWDDEE; -.
DR OrthoDB; 1395906at2759; -.
DR PhylomeDB; Q9SSW1; -.
DR PRO; PR:Q9SSW1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9SSW1; baseline and differential.
DR Genevisible; Q9SSW1; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; ISS:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:TAIR.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0042538; P:hyperosmotic salinity response; IEP:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0009409; P:response to cold; IEP:TAIR.
DR InterPro; IPR044653; AZF1/2/3-like.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR PANTHER; PTHR45988; PTHR45988; 1.
DR SMART; SM00355; ZnF_C2H2; 2.
DR SUPFAM; SSF57667; SSF57667; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 2.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
PE 2: Evidence at transcript level;
KW Abscisic acid signaling pathway; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..245
FT /note="Zinc finger protein AZF1"
FT /id="PRO_0000421826"
FT ZN_FING 97..119
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 164..186
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 112..141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 193..231
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..35
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 193..207
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 245 AA; 26471 MW; A342B6B68FBE64E3 CRC64;
MALETLNSPT ATTTARPLLR YREEMEPENL EQWAKRKRTK RQRFDHGHQN QETNKNLPSE
EEYLALCLLM LARGSAVQSP PLPPLPSRAS PSDHRDYKCT VCGKSFSSYQ ALGGHKTSHR
KPTNTSITSG NQELSNNSHS NSGSVVINVT VNTGNGVSQS GKIHTCSICF KSFASGQALG
GHKRCHYDGG NNGNGNGSSS NSVELVAGSD VSDVDNERWS EESAIGGHRG FDLNLPADQV
SVTTS
